We narrowed to 22,815 results for: Fus

1. pET30a-GalK-USP-ET64

   Plasmid

   #234662

   Purpose

   Expression an ELP-tagged fusion enzyme of GalK and AtUSP

   Depositor

   Xianwei Liu

   Article

   Wu et al Chembiochem. 2025 Jan 13:e202401005. doi

   Inserts

   galactokinase
   UDP-sugar pyrophosphorylase (USP Mustard Weed)
   

   Use

   Tags

   ELP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRSFDUET-1_VNp-anti-Lysozyme fAb

   Plasmid

   #214951

   Purpose

   Bacterial expression of anti-chicken lysozyme (folded) Fab light and heavy chain VNp fusions

   Depositor

   Dan Mulvihill

   Article

   Baker et al Open Biol. 2025 Feb;15(2):240229. doi: 1

   Inserts

   VNp-anti-Lysozyme Fab heavy chain fusion
   VNp-anti-Lysozyme Fab light chain fusion
   

   Use

   Tags

   VNp

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEM.H10L

   Plasmid

   #198683

   Purpose

   An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

   Depositor

   Paola Branduardi

   Article

   Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

   Insert

   XI1_UP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEM.H10R

   Plasmid

   #198684

   Purpose

   An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

   Depositor

   Paola Branduardi

   Article

   Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

   Insert

   XI1_DW
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEM.H09R

   Plasmid

   #198681

   Purpose

   An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

   Depositor

   Paola Branduardi

   Article

   Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

   Insert

   XII4_UP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEM.H09L

   Plasmid

   #198682

   Purpose

   An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

   Depositor

   Paola Branduardi

   Article

   Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

   Insert

   XII4_DW
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEM.H06R

   Plasmid

   #198676

   Purpose

   An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

   Depositor

   Paola Branduardi

   Article

   Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

   Insert

   XII5_DW
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEM.H03L

   Plasmid

   #198669

   Purpose

   An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

   Depositor

   Paola Branduardi

   Article

   Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

   Insert

   XII2_UP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEM.H01R

   Plasmid

   #198666

   Purpose

   An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

   Depositor

   Paola Branduardi

   Article

   Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

   Insert

   X3_DW
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEM.H11R

    Plasmid

    #198686

    Purpose

    An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

    Depositor

    Paola Branduardi

    Article

    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

    Insert

    XII1_DW
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEM.H11L

    Plasmid

    #198685

    Purpose

    An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

    Depositor

    Paola Branduardi

    Article

    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

    Insert

    XII1_UP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEM.H08R

    Plasmid

    #198680

    Purpose

    An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

    Depositor

    Paola Branduardi

    Article

    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

    Insert

    XI5_DW
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEM.H08L

    Plasmid

    #198679

    Purpose

    An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

    Depositor

    Paola Branduardi

    Article

    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

    Insert

    XI5_UP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEM.H07R

    Plasmid

    #198678

    Purpose

    An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

    Depositor

    Paola Branduardi

    Article

    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

    Insert

    X2_DW
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEM.H07L

    Plasmid

    #198677

    Purpose

    An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

    Depositor

    Paola Branduardi

    Article

    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

    Insert

    X2_UP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEM.H06L

    Plasmid

    #198675

    Purpose

    An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

    Depositor

    Paola Branduardi

    Article

    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

    Insert

    XII5_UP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEM.H05R

    Plasmid

    #198674

    Purpose

    An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

    Depositor

    Paola Branduardi

    Article

    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

    Insert

    XI3_DW
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEM.H05L

    Plasmid

    #198673

    Purpose

    An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

    Depositor

    Paola Branduardi

    Article

    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

    Insert

    XI3_UP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEM.H04R

    Plasmid

    #198672

    Purpose

    An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

    Depositor

    Paola Branduardi

    Article

    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

    Insert

    X4_DW
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEM.H04L

    Plasmid

    #198671

    Purpose

    An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

    Depositor

    Paola Branduardi

    Article

    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

    Insert

    X4_UP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pEM.H03R

    Plasmid

    #198670

    Purpose

    An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

    Depositor

    Paola Branduardi

    Article

    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

    Insert

    XII2_DW
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEM.H02R

    Plasmid

    #198668

    Purpose

    An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

    Depositor

    Paola Branduardi

    Article

    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

    Insert

    XI2_DW
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEM.H02L

    Plasmid

    #198667

    Purpose

    An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

    Depositor

    Paola Branduardi

    Article

    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

    Insert

    XI2_UP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEM.H01L

    Plasmid

    #198665

    Purpose

    An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

    Depositor

    Paola Branduardi

    Article

    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

    Insert

    X3_UP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pVH616

    Plasmid

    #169605

    Purpose

    Tier-2 vector encoding PmPGK1-driven Fluc and PCREm4-driven Nluc expression (PmPGK1-Fluc-pA::A2-pA::PCREm4-Nluc-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven FLuc Luciferase expression cassette and cAMP response element-controlled NanoLuc Luciferase expression cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PCREm4 / PPGK

    Available since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pVH270-Tier1-OTtgO2-PCMVmin2-SEAP-p2A-iRFP670

    Plasmid

    #169592

    Purpose

    Tier-1 vector encoding PTtgO2-driven SEAP-p2A-iRFP670 expression (OTtgO2-PCMVmin-2-SEAP-p2A-iRFP670-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    phloretin-controlled SEAP and iRFP expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TtgO2-PCMVmin-2

    Available since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pVH266-Tier1-OTetO7-PCMVmin2-SEAP-sTRSV

    Plasmid

    #169589

    Purpose

    Tier-1 vector encoding PTetO7-driven SEAP expression with a 3'UTR sTRSV ribozyme (OTetO7-PCMVmin-2-SEAP-3'UTRsTRSV-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    tetracycline-controlled SEAP expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO7-PCMVmin-2

    Available since

    July 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pTS2343_Tier3(SB)-YPet_Blast

    Plasmid

    #169641

    Purpose

    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a YPet marker and BlastR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-YPet-p2A-BlastR-pA-3'ITR)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PRPBSA-driven YPet and BlastR expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PRPBSA

    Available since

    July 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pTS2344_Tier3(SB)-mRuby2_Blast

    Plasmid

    #169642

    Purpose

    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a mRuby marker and BlastR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-mRuby2-p2A-BlastR-pA-3'ITR)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PRPBSA-driven mRuby2 and BlastR expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PRPBSA

    Available since

    July 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pTS2339_Tier3(SB)-Ruby_Puro

    Plasmid

    #169637

    Purpose

    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a mRuby2 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-mRuby2-p2A-PuroR-pA-3'ITR)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PRPBSA-driven mRuby2 and PuroR expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PRPBSA

    Available since

    July 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pTS2354_Tier3(PB)-Ruby_PuroR

    Plasmid

    #169653

    Purpose

    Tier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a mRuby2 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-mRuby2-p2A-PuroR-pA-3'ITR)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PRPBSA-driven mRuby2 and PuroR expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PRPBSA

    Available since

    July 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pTS2355_Tier3(PB)-iRFP_PuroR

    Plasmid

    #169654

    Purpose

    Tier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a iRFP670 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-iRFP670-p2A-PuroR-pA-3'ITR)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PRPBSA-driven iRFP670 and PuroR expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PRPBSA

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pTS2357_Tier3(PB)-TagBFP_BlastR

    Plasmid

    #169656

    Purpose

    Tier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a mTagBFP2 marker and BlastR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-mTagBFP2-p2A-BlastR-pA-3'ITR)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PRPBSA-driven mTagBFP2 and BlastR expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PRPBSA

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pVH614

    Plasmid

    #169603

    Purpose

    Tier-2 vector encoding PmPGK1-driven Fluc and PCREm4-driven Nluc expression (PmPGK1-Fluc-pA::PCREm4-Nluc-pA::A3-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven FLuc Luciferase expression cassette and cAMP response element-controlled NanoLuc Luciferase expression cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PCREm4 / PPGK

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pVH267-Tier1-OTetO7-PCMVmin2-SEAP-env140

    Plasmid

    #169590

    Purpose

    Tier-1 vector encoding PTetO7-driven SEAP expression with a 3'UTR env140 ribozyme (OTetO7-PCMVmin-2-SEAP-3'UTRenv140-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    tetracycline-controlled SEAP expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO7-PCMVmin-2

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pVH269-Tier1-OVanO2-PCMVmin2-SEAP-p2A-iRFP670

    Plasmid

    #169667

    Purpose

    Tier-1 vector encoding PVanO2-driven SEAP-p2A-iRFP670 expression (OVanO2-PCMVmin-2-SEAP-p2A-iRFP670-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    vanillic acid-controlled SEAP and iRFP expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    VanO2-PCMVmin

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pVH321-2-Tier1-PhCMV-NLS-dCas9-3xNLS

    Plasmid

    #169596

    Purpose

    Tier-1 vector encoding PhCMV-driven NLS-dCas9-3xNLS expression (PhCMV-NLS-dCas9-3xNLS-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    dead S.pyogenes Cas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pTS2406-Tier1-PhCMV-NTEV

    Plasmid

    #169501

    Purpose

    Tier-1 vector encoding PhCMV-driven N-terminal fragment of a split tabacco etch virus protease splitTEV (PhCMV-N-TEV-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven N-terminal fragment of split tobacco etch virus protease
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pTS1024-Tier1-PRPBSA-YPet-p2A-PuroR

    Plasmid

    #169499

    Purpose

    Tier-1 vector encoding PRPBSA-driven YPet in combination with a marker for puromycine selection (PRPBSA-YPet-p2A-PuroR-pA)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PRPBSA-driven YPet-P2aA-PuroR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PRBSA

    Available since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pTS1047-Tier1-PhCMV-YPet-p2A-PuroR

    Plasmid

    #169498

    Purpose

    Tier-1 vector encoding PhCMV-driven YPet in combination with a marker for puromycin selection (PhCMV-YPet-p2A-PuroR-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven YPet-P2aA-PuroR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pV5endoNA2

    Plasmid

    #174729

    Purpose

    Expresses V5-endoNA2 fusion protein for detection of polysialic acid. The endoNA2 is a catalytically inactive form of endosialidase from bacteriophage PK1A2.

    Depositor

    Jukka Finne

    Article

    Lehti et al Mol Cancer Ther. 2021 Jul 26. pii: 1535-

    Insert

    V5 epitope tag
    

    Use

    Tags

    Histag

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pZA-YFP-R5

    Plasmid

    #106257

    Purpose

    Expresses Venus YFP fused with silaffin-R5 silica affinity peptide under the control of TetO

    Depositor

    Urartu Seker

    Article

    Olmez et al ACS Omega. 2018 Jan 31;3(1):585-594. doi

    Insert

    Yellow fluorescent protein
    

    Use

    Tags

    Added silaffin R5 peptide to C terminus of YFP af…

    Expression

    Bacterial

    Mutation

    Promoter

    pL-TetO

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pGWB425

    Plasmid

    #74819

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with T7 (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pET30-GBF-E6N

    Plasmid

    #21699

    Depositor

    James Baleja

    Article

    Liu et al J Mol Biol. 2009 Mar 6. 386(4):1123-37.

    Insert

    Protein G domain B1 fused N-terminal zinc binding domain of human papillomavirus type 16 (residues 2-74) (E6 Human papillomavirus type 16)
    

    Use

    Tags

    Protein G B1 domain

    Expression

    Bacterial

    Mutation

    GB1 (1-56aa) fusion tag with mutations at three p…

    Promoter

    Available since

    July 28, 2009

    Availability

    Academic Institutions and Nonprofits only

45. pHLmMBP-3

    Plasmid

    #72345

    Purpose

    Mammalian expression of secreted N-terminally 8His-tagged mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

    Depositor

    Luca Jovine

    Article

    Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

    Type

    Empty backbone

    Use

    Tags

    6His-tag, 8His-tag, HA-tag, Strep-tag II, and mMB…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV enhancer + chicken beta-actin promoter

    Available since

    Feb. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. hRad51(S208E-A209D)–Cas9(D10A)

    Plasmid

    #125561

    Purpose

    Expresses the hRad51(S208E-A209D)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter

    Depositor

    David Liu

    Article

    Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

    Insert

    Rad51(S208E A209D)–Cas9(D10A) (RAD51 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. CMV-LUC2CP/ARE

    Plasmid

    #62857

    Purpose

    Splicing reporter control (no intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.

    Depositor

    Gideon Dreyfuss

    Article

    Younis et al Mol Cell Biol. 2010 Apr;30(7):1718-28. d

    Insert

    Luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    destabilizing sequences (DS) added to the C termi…

    Promoter

    CMV

    Available since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. piggyBAC-H2B FR-MQV

    Plasmid

    #194079

    Purpose

    Expresses nuclear-localized FR-MQV in mammalian cells

    Depositor

    Ralph Jimenez

    Article

    Mukherjee et al Biochemistry. 2020 Oct 6;59(39):3669-368

    Insert

    FR-MQV
    

    Use

    Tags

    Histone H2B

    Expression

    Mammalian

    Mutation

    Methionine 42 to Glutamine, Cystiene 159 to Valin…

    Promoter

    CMV7

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. msfGFP-Cx43

    Plasmid

    #69024

    Purpose

    Expresses rat Cx43 (Gja1 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to msfGFP (FP fused to NT of connexin). CMV promoter.

    Depositor

    David Spray

    Article

    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

    Insert

    Cx43 (Gja1 Rat)
    

    Use

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. vGlut1 peptide- GST

    Plasmid

    #218677

    Purpose

    Expresses a GST fusion to vGlut peptide for immunoisolation of glutamatergic vesicles

    Depositor

    Axel Brunger

    Article

    Leitz et al Nat Protoc. 2024 Jul 2. doi: 10.1038/s41

    Insert

    vGlut (Slc17a6 Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge