159,238 results

1. pLV-UBC mTagBFP2-P2A-3xFLAG-hEEA1

   Plasmid

   #214995

   Purpose

   Express the human EndoTag (3xFLAG-EEA1) for EndoIPs. mTagBFP2 to sort for even expression levels.

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   EEA1 (EEA1 Human)
   

   Use

   Lentiviral

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. C1-MPAct-mCitrine

   Plasmid

   #155220

   Purpose

   Monitor changes in the density of membrane-proximal F-actin (MPA)

   Depositor

   Tobias Meyer

   Article

   Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

   Insert

   F-tractin (aa 9-52 of rat ITPKA) fused to mCitrine C-term tagged with the CaaX sequence derived from Kras4b
   

   Use

   Tags

   mCitrine

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRDA_887

   Plasmid

   #201164

   Purpose

   Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' p65 and NLS

   Depositor

   John Doench

   Article

   Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

   Insert

   Puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EFS

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-GRAB-rDA3m

   Plasmid

   #208702

   Purpose

   Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3m in neurons

   Depositor

   Yulong Li

   Article

   Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Insert

   GPCR activation based dopamine (DA) sensor GRAB_rDA3m
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA5/FRT/TO_H2B_HaloTag7

   Plasmid

   #169329

   Purpose

   Expression of H2B-HaloTag7 in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   H2B-HaloTag7 (H2BC21 Human)
   

   Use

   Tags

   HaloTag7

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. plenti.CamKII.(cyto).iATPSnFR2.A95A.A119L.miRFP670nano3

   Plasmid

   #209725

   Purpose

   Expresses cytosolic low affinity ratiometric ATP sensor

   Depositor

   Timothy Ryan

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   (cyto).iATPSnFR2.A95A.A119L.miRFP670nano3
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CamKII

   Available since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-MbPylRS(DiZPK)

   Plasmid

   #91706

   Purpose

   express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in mammalian cells to incorporate the photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into amber codon site on a protein of interest

   Depositor

   P. Chen

   Article

   Lin et al Angew Chem Int Ed Engl. 2013 Dec 23;52(5

   Inserts

   pylS
   pylT
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   T810G, C813T, C820G, T821C, T937A, A1046T, G1257A

   Promoter

   Available since

   July 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. APEX2-NLS

   Plasmid

   #124617

   Purpose

   proximity biotinylation

   Depositor

   Alice Ting

   Article

   Kaewsapsak et al Elife. 2017 Dec 14;6. doi: 10.7554/eLife

   Insert

   APEX2-NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pWM_12x601_25bpLinker

   Plasmid

   #157788

   Purpose

   EcoRV digestion produces 12x601 chromatin assembly construct with 25 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   12x601_25bp linker
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV/mIba1.GFP.WPRE.miR-9.T.miR-129-2-3p.T.SV40pA

    Plasmid

    #190163

    Purpose

    Microglia-targeted transgene expression.

    Depositor

    Hirokazu Hirai

    Article

    Okada et al Commun Biol. 2022 Nov 11;5(1):1224. doi:

    Inserts

    mIba1 promoter, 1.7-kb, microglia-specific promoter
    miR-9-Tx4, 4 repeated miRNA target sites for miR-9
    miR-129-2-3p-Tx4, 4 repeated miRNA target sites for miR-129-2-3p
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-Cre

    Plasmid

    #123133

    Purpose

    Mammalian expression vector for wild-type Cre.

    Depositor

    David Liu

    Article

    Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

    Insert

    Cre recombinase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. Flag-HA-USP11

    Plasmid

    #22566

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP11 (USP11 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-syn-flex-iGluSnFR4s-NGR-WPRE

    Plasmid

    #234441

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4s-NGR
    

    Use

    AAV and Cre/Lox

    Tags

    IgK chain and Myc epi tag-NGR GPI anchor

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV-beta-catenin deltaN90

    Plasmid

    #36985

    Depositor

    Bob Weinberg

    Article

    Guo et al Cell. 2012 Mar 2;148(5):1015-28.

    Insert

    beta-catenin deltaN90 (CTNNB1 Human)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 1-90 (Constitutively Acti…

    Promoter

    EF1a

    Available since

    Sept. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGES201

    Plasmid

    #190197

    Purpose

    For CRISPR-Cas9 mediated gene editing in soybean, soybean elongation factor 1A promoter (pM4)-Cas9, GmU6-sgRNA, Basta selection

    Depositor

    Yuefeng Guan

    Article

    Bai et al Plant Biotechnol J. 2020 Mar;18(3):721-7

    Insert

    spCas9
    

    Use

    CRISPR

    Tags

    3xFlag

    Expression

    Plant

    Mutation

    plant-codon optimized

    Promoter

    Glycine max elongation factor 1A(pM4)

    Available since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHyperTEV60

    Plasmid

    #228496

    Purpose

    10xHis-SUMO (Smt3) tagged HyperTEV60 engineered protease (E. coli based protein expression)

    Depositor

    Richard Baker

    Insert

    TEV Protease
    

    Use

    Tags

    10xHis-SUMO(Smt3)

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-F-RAM-d2tTA-TRE-mKate2

    Plasmid

    #140274

    Purpose

    F-RAM, the Fos-dependent reporter

    Depositor

    Yingxi Lin

    Article

    Sun et al Cell. 2020 Apr 16;181(2):410-423.e17. do

    Insert

    mKate2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    TRE

    Available since

    July 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. mCherry-Ezrin-N-14

    Plasmid

    #55043

    Purpose

    Localization: Focal Adhesions/Membrane, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Ezrin (EZR Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHCMV_FLUAV_HA

    Plasmid

    #207273

    Purpose

    Mammalian expression of Influenza A virus HA protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    FLUAV_HA (HA Influenza A virus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHCMV_DENV3_PrME

    Plasmid

    #207265

    Purpose

    Mammalian expression of Dengue virus 3 PrME protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    DENV3_PrME (POLY Dengue virus 3)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge