We narrowed to 15,117 results for: pET

1. pRM049 His-TEV-Cas9 triNLS 2C

   Plasmid

   #196245

   Purpose

   Expression of NLS-rich Cas9 (2 Cys residues, mammalian codon optimized)

   Depositor

   Ross Wilson

   Article

   Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   HA, His6 (N terminal on backbone), NLS, Nucleopla…

   Expression

   Bacterial

   Available Since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. FGFR1_HUMAN_D0

   Plasmid

   #79719

   Purpose

   This plasmid encodes the kinase domain of FGFR1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Nicholas Levinson, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   FGFR1 (FGFR1 Human)
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pEMS1401

   Plasmid

   #29198

   Purpose

   High copy homologous recombination plasmid for gene targeting at the mouse Hprt locus containing Ple67 (FEV MiniPromoter) driving an EGFP reporter

   Depositor

   Elizabeth Simpson

   Article

   Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

   Insert

   Ple67 (FEV Human)
   

   Use

   Pleiades promoter project [sic, pleaides plieades]

   Tags

   EGFP-NLS

   Available Since

   Nov. 15, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. MK01_HUMAN_D0

   Plasmid

   #79713

   Purpose

   This plasmid encodes the kinase domain of MK01. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Nicholas Levinson, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   MK01 (MAPK1 Human)
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pEMS1538

   Plasmid

   #29261

   Purpose

   High copy homologous recombination plasmid for gene targeting at the mouse Hprt locus containing Ple67 (FEV MiniPromoter) driving a lacZ reporter

   Depositor

   Elizabeth Simpson

   Article

   Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

   Insert

   Ple67 (FEV Human)
   

   Use

   Pleiades promoter project [sic, pleaides plieades]

   Tags

   Intron-LacZ-NLS

   Available Since

   Oct. 28, 2011

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pMBP-HsSyx3

   Plasmid

   #179287

   Purpose

   E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + mature maltose binding protein + TEV protease cleavage site

   Depositor

   Debra Hansen

   Article

   Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

   Insert

   Syx
   

   Tags

   His6 + mature maltose binding protein + TEV prote…

   Expression

   Bacterial

   Mutation

   residues 393-792 from accession number NP_0010361…

   Promoter

   T7

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMBP-HsSyx-375-792

   Plasmid

   #179291

   Purpose

   E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 375-792) with N-terminal His6 + mature maltose binding protein + TEV protease cleavage site

   Depositor

   Debra Hansen

   Article

   Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

   Insert

   Syx
   

   Tags

   His6 + mature maltose binding protein + TEV prote…

   Expression

   Bacterial

   Mutation

   residues 375-792 from accession number NP_0010361…

   Promoter

   T7

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGLU_115

   Plasmid

   #225795

   Purpose

   RS421 library variant 14067

   Depositor

   Nili Ostrov

   Insert

   14067_[Tn5]
   

   Use

   Synthetic Biology

   Mutation

   N/A

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGLU_79

   Plasmid

   #225780

   Purpose

   RS421 library variant 14067

   Depositor

   Nili Ostrov

   Insert

   14067_[abr]
   

   Use

   Synthetic Biology

   Mutation

   N/A

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGLU_97

    Plasmid

    #225810

    Purpose

    RS421 library variant 14067

    Depositor

    Nili Ostrov

    Insert

    14067_[Himar]
    

    Use

    Synthetic Biology

    Mutation

    N/A

    Available Since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJK635

    Plasmid

    #72473

    Purpose

    pET28a modified to contain TEV protease fusion tag instead of T7 tag

    Depositor

    T. J. Kappock

    Type

    Empty backbone

    Tags

    His6 and TEV protease cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGFP(C3)-Vinculin

    Plasmid

    #30312

    Depositor

    Klaus Hahn

    Insert

    Vinculin (Vcl Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    F633S, V828E, L936P in mouse viniculin (NM_009502…

    Available Since

    July 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. RP1B-PP1gamma

    Plasmid

    #51770

    Purpose

    Expresses PP1 gamma residues 7-323

    Depositor

    Wolfgang Peti

    Insert

    pp1 gamma
    

    Tags

    His, TEV protease site, and Thio-6

    Expression

    Bacterial

    Mutation

    Contains PP1 gamma residues 7-323

    Promoter

    T7-lac

    Available Since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

14. RP1B-PP1beta

    Plasmid

    #51769

    Purpose

    Expresses PP1 beta residues 6-327

    Depositor

    Wolfgang Peti

    Insert

    pp1 beta
    

    Tags

    His, TEV protease site, and Thio-6

    Expression

    Bacterial

    Mutation

    Contains PP1 beta residues 6-327

    Promoter

    T7-lac

    Available Since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

15. U6+27-cxcr4

    Plasmid

    #22498

    Depositor

    Phil Sharp

    Article

    Ebert et al Nat Methods. 2007 Sep . 4(9):721-6.

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Nov. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

16. U6+27-20

    Plasmid

    #21977

    Depositor

    Phil Sharp

    Article

    Ebert et al Nat Methods. 2007 Sep . 4(9):721-6.

    Insert

    U6 miR-20 sponge
    

    Expression

    Mammalian

    Available Since

    Nov. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

17. U6+27-16

    Plasmid

    #21975

    Depositor

    Phil Sharp

    Article

    Ebert et al Nat Methods. 2007 Sep . 4(9):721-6.

    Insert

    U6 miR-16 sponge
    

    Expression

    Mammalian

    Available Since

    Nov. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pRL-TK CXCR4 2x

    Plasmid

    #11306

    Depositor

    Phil Sharp

    Article

    Doench et al Genes Dev. 2003 Feb 15. 17(4):438-42.

    Insert

    2 bulged bind sites for CXCR4 siRNA antisense
    

    Use

    Luciferase

    Tags

    Rr-luc

    Available Since

    Feb. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRL-TK CXCR4 4x

    Plasmid

    #11307

    Depositor

    Phil Sharp

    Article

    Doench et al Genes Dev. 2003 Feb 15. 17(4):438-42.

    Insert

    4 bulged bind sites for CXCR4 siRNA antisense
    

    Use

    Luciferase

    Tags

    Rr-luc

    Available Since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSLAX13 (CT#232)

    Plasmid

    #13918

    Depositor

    Cliff Tabin

    Article

    Logan et al Methods. 1998 Apr . 14(4):407-20.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Feb. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

21. pRL-TK CXCR4 6x

    Plasmid

    #11308

    Depositor

    Phil Sharp

    Article

    Doench et al Genes Dev. 2003 Feb 15. 17(4):438-42.

    Insert

    6 bulged bind sites for CXCR4 siRNA antisense
    

    Use

    Luciferase

    Tags

    Rr-luc

    Available Since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRL-TK CXCR4 1P

    Plasmid

    #11309

    Depositor

    Phil Sharp

    Article

    Doench et al Genes Dev. 2003 Feb 15. 17(4):438-42.

    Insert

    1 perfect bind site for CXCR4 siRNA antisense
    

    Use

    Luciferase

    Tags

    Rr-luc

    Available Since

    Feb. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSLAX13 HA (CT#233)

    Plasmid

    #14027

    Depositor

    Cliff Tabin

    Article

    Logan et al Methods. 1998 Apr . 14(4):407-20.

    Type

    Empty backbone

    Tags

    3x HA

    Expression

    Bacterial

    Available Since

    Feb. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

24. pGL3 CXCR4 1P

    Plasmid

    #11310

    Depositor

    Phil Sharp

    Article

    Doench et al Genes Dev. 2003 Feb 15. 17(4):438-42.

    Insert

    1 perfect binding site for CXCR4 siRNA antisense
    

    Use

    Luciferase

    Tags

    Pp-luc

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGL3 CXCR4 2P

    Plasmid

    #11311

    Depositor

    Phil Sharp

    Article

    Doench et al Genes Dev. 2003 Feb 15. 17(4):438-42.

    Insert

    2 perfect binding sites for CXCR4 siRNA antisense
    

    Use

    Luciferase

    Tags

    Pp-luc

    Available Since

    Feb. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. U6+27-16pf

    Plasmid

    #21976

    Depositor

    Phil Sharp

    Article

    Ebert et al Nat Methods. 2007 Sep . 4(9):721-6.

    Insert

    U6 miR-16 perfect
    

    Expression

    Mammalian

    Available Since

    Nov. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

27. pGL3 CXCR4 3P

    Plasmid

    #11312

    Depositor

    Phil Sharp

    Article

    Doench et al Genes Dev. 2003 Feb 15. 17(4):438-42.

    Insert

    3 perfect binding sites for CXCR4 siRNA antisense
    

    Use

    Luciferase

    Tags

    Pp-luc

    Available Since

    Feb. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PKA1248

    Plasmid

    #239369

    Purpose

    S.cerevisiae Sla1-SH3#1-3 (5-413) in pGEX6P1

    Depositor

    Kathryn Ayscough

    Insert

    SLA1 (amino acids 5-413)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    July 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. PKA1247

    Plasmid

    #239368

    Purpose

    S.cerevisiae Sla1-SH3#1-2 (5-131) in pGEX6P1

    Depositor

    Kathryn Ayscough

    Insert

    SLA1 (5-131)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    July 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. U6+27-20pf

    Plasmid

    #21978

    Depositor

    Phil Sharp

    Article

    Ebert et al Nat Methods. 2007 Sep . 4(9):721-6.

    Insert

    U6 miR-20 perfect
    

    Expression

    Mammalian

    Available Since

    Nov. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

31. pKMyc

    Plasmid

    #19400

    Depositor

    Ian Macara

    Article

    Joberty et al Nat Cell Biol 2000 Aug;2(8):531-9.

    Type

    Empty backbone

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    April 2, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. CMV-d2eGFP-21

    Plasmid

    #21972

    Depositor

    Phil Sharp

    Article

    Ebert et al Nat Methods. 2007 Sep . 4(9):721-6.

    Insert

    CMV d2eGFP miR-21 sponge
    

    Expression

    Mammalian

    Available Since

    Nov. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

33. CMV-d2eGFP-cxcr4

    Plasmid

    #21967

    Depositor

    Phil Sharp

    Article

    Ebert et al Nat Methods. 2007 Sep . 4(9):721-6.

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Nov. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

34. CMV-d2eGFP-16

    Plasmid

    #22442

    Depositor

    Phil Sharp

    Article

    Ebert et al Nat Methods. 2007 Sep . 4(9):721-6.

    Insert

    CMV d2eGFP miR-16 sponge
    

    Expression

    Mammalian

    Available Since

    Nov. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

35. CMV-d2eGFP-20

    Plasmid

    #21970

    Depositor

    Phil Sharp

    Article

    Ebert et al Nat Methods. 2007 Sep . 4(9):721-6.

    Insert

    CMV d2eGFP miR-20 sponge
    

    Expression

    Mammalian

    Available Since

    Dec. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

36. pMG207

    Plasmid

    #39273

    Depositor

    Peter Kast

    Article

    Gamper et al Biotechniques. 2005 Mar;38(3):405-8.

    Type

    Empty backbone

    Use

    E coli gene targeting

    Available Since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. CMV-d2eGFP-20pf

    Plasmid

    #21971

    Depositor

    Phil Sharp

    Article

    Ebert et al Nat Methods. 2007 Sep . 4(9):721-6.

    Insert

    CMV d2eGFP miR-20 perfect
    

    Expression

    Mammalian

    Available Since

    Nov. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

38. CMV-d2eGFP-16pf

    Plasmid

    #21969

    Depositor

    Phil Sharp

    Article

    Ebert et al Nat Methods. 2007 Sep . 4(9):721-6.

    Insert

    CMV d2eGFP miR-16 perfect
    

    Expression

    Mammalian

    Available Since

    Nov. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

39. pFredA

    Plasmid

    #16581

    Depositor

    Bert Vogelstein

    Article

    Jallepalli et al Cell. 2001 May 18. 105(4):445-57.

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    March 10, 2008

    Availability

    Academic Institutions and Nonprofits only

40. pFredB

    Plasmid

    #16582

    Depositor

    Bert Vogelstein

    Article

    Jallepalli et al Cell. 2001 May 18. 105(4):445-57.

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    March 10, 2008

    Availability

    Academic Institutions and Nonprofits only

41. pSLAX13 Flag (CT#231)

    Plasmid

    #14026

    Depositor

    Cliff Tabin

    Article

    Logan et al Methods. 1998 Apr . 14(4):407-20.

    Type

    Empty backbone

    Tags

    Flag

    Expression

    Bacterial

    Available Since

    Feb. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

42. PKA1317

    Plasmid

    #239371

    Purpose

    S.cerevisiae Sla1-SH3#1-2 (5-131) W108A (mutation preventing binding to 2nd SH3)

    Depositor

    Kathryn Ayscough

    Insert

    SLA1 (5-131)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    W108A

    Available Since

    July 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. PKA1279

    Plasmid

    #239375

    Purpose

    GST-Las17 (300-422) P388A

    Depositor

    Kathryn Ayscough

    Insert

    Las17 (amino acids 300-422)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Proline 388 to Alanine

    Available Since

    July 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pSLAX13 HA His (CT#244)

    Plasmid

    #14029

    Depositor

    Cliff Tabin

    Article

    Logan et al Methods. 1998 Apr . 14(4):407-20.

    Type

    Empty backbone

    Tags

    3x HA and His

    Expression

    Bacterial

    Available Since

    Feb. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

45. pCT02

    Plasmid

    #212152

    Purpose

    Plasmid expressing 6x His tagged FAST-PETase under inducible promoter PT7 control.

    Depositor

    Kang Zhou

    Insert

    FAST-PETase
    

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    S121E, D186H, R224Q, N233K, R280A

    Promoter

    PT7

    Available Since

    March 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. 9AVA

    Plasmid

    #220884

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    TREX1 (TREX1 Human)
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 20, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

47. CMV-d2eGFP-empty

    Plasmid

    #26164

    Depositor

    Phil Sharp

    Article

    Ebert et al Nat Methods. 2007 Sep . 4(9):721-6.

    Insert

    
    

    Expression

    Mammalian

    Mutation

    …ccgcCTCGAG[sponge sites]GGGCCCgttt… became …ccgc…

    Available Since

    Aug. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. KE-005

    Plasmid

    #133440

    Purpose

    Human elF4A1 residues 1-406, N-term His tag, thrombin cleavage, in pET21 vector backbone.

    Depositor

    Gerhard Wagner

    Insert

    EIF4A1 (EIF4A1 Human)
    

    Expression

    Bacterial

    Available Since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pMLM292

    Plasmid

    #21873

    Depositor

    Keith Joung

    Article

    Foley et al Nat Protoc. 2009. 4(12):1855-67.

    Type

    Empty backbone

    Use

    Zebrafish expression

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Nov. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pMLM290

    Plasmid

    #21872

    Depositor

    Keith Joung

    Article

    Foley et al Nat Protoc. 2009. 4(12):1855-67.

    Type

    Empty backbone

    Use

    Zebrafish expression

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Nov. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge