We narrowed to 2,695 results for: GUS

1. pAMS823 His6-MBP-3C-ORF2p (238-1061, ORFeus-Hs) in pET41

   Plasmid

   #213024

   Purpose

   Expresses human LINE-1 ORF2p Core (238-1061) in E. coli as an N-MBP fusion

   Depositor

   Oliver Weichenrieder, Martin S. Taylor

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   LINE-1 ORF2p (238-1061)
   

   Tags

   His6-MBP-3C

   Expression

   Bacterial

   Promoter

   T7 / Lac

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMT646 human L1 ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro

   Plasmid

   #213026

   Purpose

   Expresses full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

   Depositor

   Martin S. Taylor

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   Human LINE-1 (ORFeus-Hs codon optimized sequence) with ORF2-3xFlag
   

   Tags

   3xFlag (ORF2p)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLD564 human L1 ORF2p-3xFlag (L1RP, CMV promoter) in pCEP4 Puro

   Plasmid

   #213030

   Purpose

   Expresses full length human LINE-1 in human cells (native L1RP sequence), with a C-terminal 3xFlag tag on ORF2

   Depositor

   Martin S. Taylor

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   Human LINE-1 (L1RP Sequence) with 5' UTR and ORF2-3xFlag
   

   Tags

   3xFlag (ORF2p)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-SARS2SΔC-H2gp41

   Plasmid

   #170389

   Purpose

   Generation of SARS-CoV-2 spike pseudotyped lentivirus

   Depositor

   Nir Hacohen

   Article

   Filbin et al Cell Rep Med. 2021 May 18;2(5):100287. d

   Insert

   SARS-CoV-2 Spike (S SARS-CoV-2)
   

   Tags

   HIV gp41

   Expression

   Mammalian

   Mutation

   Deletion of C terminal and fusion with H2 from HI…

   Promoter

   CMV

   Available Since

   June 23, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pRT006.3 L1 dual-luciferase AI retrotransposition reporter

   Plasmid

   #213031

   Purpose

   Bi-directional luciferase antisense intron (firefly fluc AI) human LINE-1 retrotransposition reporter (ORFeus-Hs sequence) for sleeping beauty integration

   Depositor

   Donna Romero, Martin S. Taylor

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   Tet-On Human LINE-1 (ORFeus-Hs) Firefly + Renilla Luciferase Antisense Intron dual retrotransposition marker
   

   Use

   Luciferase

   Tags

   Firefly luciferase antisense intron (3' UTR)

   Expression

   Mammalian

   Promoter

   pTRE bidirectional tet-on

   Available Since

   July 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMT692 ORF2p-3C-3xF (1-1275, ORFeus-Hs) in pDARMO-PolH2.1

   Plasmid

   #213025

   Purpose

   Expresses full length human LINE-1 ORF2p Core (1-1275) with a C-terminal 3xFlag tag for baculovirus production in insect cells

   Depositor

   Martin S. Taylor

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   LINE-1 ORF2p (1-1275)
   

   Tags

   3C-3xFlag (ORF2p)

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. CoV2-Spike-D614G-Beta

   Plasmid

   #177962

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (Beta: K417N, E484K, N501Y, D614G)

   Depositor

   Melanie Ott

   Article

   Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

   Insert

   SARS-CoV-2 Spike protein (Beta: K417N, E484K, N501Y, D614G) (S )
   

   Expression

   Mammalian

   Mutation

   K417N, E484K, N501Y, D614G

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. CoV2-Spike-D614G-Epsilon

   Plasmid

   #177964

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (Epsilon: S13I, W152C, L452R, D614G)

   Depositor

   Melanie Ott

   Article

   Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

   Insert

   SARS-CoV-2 Spike protein (Epsilon: S13I, W152C, L452R, D614G) (S )
   

   Expression

   Mammalian

   Mutation

   S13I, W152C, L452R, D614G

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CoV2-Spike-D614G-K417N

   Plasmid

   #177969

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, K417N)

   Depositor

   Melanie Ott

   Article

   Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

   Insert

   SARS-CoV-2 Spike protein (D614G, K417N) (S )
   

   Expression

   Mammalian

   Mutation

   D614G, K417N

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CoV2-Spike-D614G-N439K

    Plasmid

    #177970

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, N439K)

    Depositor

    Melanie Ott

    Article

    Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

    Insert

    SARS-CoV-2 Spike protein (D614G, N439K) (S )
    

    Expression

    Mammalian

    Mutation

    D614G, N439K

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CoV2-Spike-D614G-L452R

    Plasmid

    #177971

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, L452R)

    Depositor

    Melanie Ott

    Article

    Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

    Insert

    SARS-CoV-2 Spike protein (D614G, L452R) (S )
    

    Expression

    Mammalian

    Mutation

    D614G, L452R

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CoV2-Spike-D614G-S477N

    Plasmid

    #177972

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, S477N)

    Depositor

    Melanie Ott

    Article

    Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

    Insert

    SARS-CoV-2 Spike protein (D614G, S477N) (S )
    

    Expression

    Mammalian

    Mutation

    D614G, S477N

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CoV2-Spike-D614G-N501Y

    Plasmid

    #177975

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, N501Y)

    Depositor

    Melanie Ott

    Article

    Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

    Insert

    SARS-CoV-2 Spike protein (D614G, N501Y) (S )
    

    Expression

    Mammalian

    Mutation

    D614G, N501Y

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CoV2-Spike-D614G-S13I

    Plasmid

    #177965

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, S13I)

    Depositor

    Melanie Ott

    Article

    Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

    Insert

    SARS-CoV-2 Spike protein (D614G, S13I) (S )
    

    Expression

    Mammalian

    Mutation

    D614G, S13I

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CoV2-Spike-D614G-T20N

    Plasmid

    #177966

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, T20N)

    Depositor

    Melanie Ott

    Article

    Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

    Insert

    SARS-CoV-2 Spike protein (D614G, T20N) (S )
    

    Expression

    Mammalian

    Mutation

    D614G, T20N

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CoV2-Spike-D614G-P26S

    Plasmid

    #177967

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, P26S)

    Depositor

    Melanie Ott

    Article

    Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

    Insert

    SARS-CoV-2 Spike protein (D614G, P26S) (S )
    

    Expression

    Mammalian

    Mutation

    D614G, P26S

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CoV2-Spike-D614G-W152C

    Plasmid

    #177968

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, W152C)

    Depositor

    Melanie Ott

    Article

    Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

    Insert

    SARS-CoV-2 Spike protein (D614G, W152C) (S )
    

    Expression

    Mammalian

    Mutation

    D614G, W152C

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CoV2-Spike-D614G-P681H

    Plasmid

    #177973

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, P681H)

    Depositor

    Melanie Ott

    Article

    Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

    Insert

    SARS-CoV-2 Spike protein (D614G, P681H) (S )
    

    Expression

    Mammalian

    Mutation

    D614G, P681H

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CoV2-Spike-D614G-D1118H

    Plasmid

    #177974

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, D1118H)

    Depositor

    Melanie Ott

    Article

    Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

    Insert

    SARS-CoV-2 Spike protein (D614G, D1118H) (S )
    

    Expression

    Mammalian

    Mutation

    D614G, D1118H

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMT647 human L1 ORF2p-3xFlag only (ORFeus-Hs, CMV promoter, monocistronic construct) in pCEP4 Puro (derivative of pMT646)

    Plasmid

    #213029

    Purpose

    Expresses human LINE-1 ORF2 only in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 ORF2p only (monocistronic, codon optimized ORFeus-Hs sequence) with ORF2-3xFlag
    

    Tags

    3xFlag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge