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We narrowed to 5,312 results for: cycs

Showing: 2601 - 2620 of 5312 results

  1. pEN527 - Rad21-AID[71-114]-eGFP-FRT-Blast-FRT targeting construct

    Plasmid
    #156452
    Purpose
    Targeting vector to introduce an AID-eGFP cassette at the mouse RAD21 (SCC1) locus using BLASTICIDIN selection. Auxin-inducible degron system. Designed to be used with sgRNA CCACGGTTCCATATTATCTG
    Depositor
    Elphege Nora
    Article
    Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:
    Insert
    RAD21, AID (Rad21 Mouse)
    Use
    Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    none
    Promoter
    Available since
    Sept. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    156452-plasmid-map-sequence-id-310171

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  3. pJEP315-pAAV-U6SaCas9gRNA(CREB)-CMV-SaCas9-DIO-pA

    Plasmid
    #113692
    Purpose
    U6 SaCas9 gRNA expression cassette containing a gRNA targeting the CREB gene, followed by a CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.
    Depositor
    Jonathan Ploski
    Article
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Inserts
    SaCas9 (NEWENTRY )
    SaCas9 gRNA Cassette (NEWENTRY )
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    NLS
    Expression
    Mutation
    Promoter
    Cytomegalo Virus(CMV)
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    113692-plasmid-map-sequence-id-229557

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  7. pJEP323-pAAV-FullH1TO-SaCa9gRNAi(SapI)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA Empty Cassette

    Plasmid
    #113700
    Purpose
    Dox-inducible H1 driven SaCas9 gRNA expression cassette without a gRNA. Followed by an EFS driven GFP-KASH in a separate reading frame. SapI can be used to clone in a gRNAs.
    Depositor
    Jonathan Ploski
    Article
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Inserts
    SaCas9 gRNA Cassete
    GFP
    Use
    AAV and CRISPR
    Tags
    KASH
    Expression
    Mutation
    Promoter
    Available since
    Dec. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    113700-plasmid-map-sequence-id-231558

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  9. pVZ_PcoaT_SQE1_LUP1

    Plasmid
    #103857
    Purpose
    Heterologous, cobalt-inducible expression of SQE1 and LUP1 from A. thaliana in Synechocystis sp. PCC6803
    Depositor
    Ilka Axmann
    Article
    Loeschcke et al PLoS One. 2017 Dec 27;12(12):e0189816. d
    Insert
    lupeol synthase 1 (LUP1 Mustard Weed)
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    inserted genes are codon optimized. 2nd amino aci…
    Promoter
    PcoaT (from Synechocystis sp. PCC 6803)
    Available since
    July 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    103857-plasmid-map-sequence-id-217123

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  12. pJEP321-pAAV-FullU6TO-SaCas9gRNAi(SapI)-CMV-TetR-P2A-GFP-KASH-WPRE-shortPA Empty Cassette

    Plasmid
    #113698
    Purpose
    Dox-inducible U6 SaCas9 gRNA expression cassette without a gRNA. Followed by an EFS driven GFP-KASH in a separate reading frame. SapI can be used to clone in gRNAs.
    Depositor
    Jonathan Ploski
    Article
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Inserts
    SaCas9 gRNA Cassete
    GFP
    Use
    AAV and CRISPR
    Tags
    KASH
    Expression
    Mutation
    Promoter
    Available since
    Feb. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    113698-plasmid-map-sequence-id-232033

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  14. pVZ_PcoaT_SQE1

    Plasmid
    #103860
    Purpose
    Heterologous, cobalt-inducible expression of SQE1 from A. thaliana in Synechocystis sp. PCC6803
    Depositor
    Ilka Axmann
    Article
    Loeschcke et al PLoS One. 2017 Dec 27;12(12):e0189816. d
    Insert
    squalene epoxidase 1 (XF1 Mustard Weed)
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    inserted gene is codon optimized. 2nd amino acid …
    Promoter
    PcoaT (from Synechocystis sp. PCC 6803)
    Available since
    Jan. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    103860-plasmid-map-sequence-id-202305

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  17. pLenti-CMV-human cGASdeltaN-OMM

    Plasmid
    #186887
    Purpose
    Expression of human cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Jonathan Kagan
    Article
    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.
    Insert
    Human cGAS truncation mutant (160-522 a.a.) with C-terminal MAVS transmembrane domain fusion (Adamts1 Synthetic, Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    N-terminal truncation, MAVS transmembrane domain …
    Promoter
    CMV
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    186887-plasmid-map-sequence-id-366443

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  18. pLenti-CMV-orangutan cGASdeltaN-OMM

    Plasmid
    #186888
    Purpose
    Expression of orangutan cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Jonathan Kagan
    Article
    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.
    Insert
    Orangutan cGAS truncation mutant (158-521 a.a.) with C-terminal MAVS transmembrane domain fusion (CGAS Synthetic, Pongo abelii)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    N-terminal truncation, MAVS transmembrane domain …
    Promoter
    CMV
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    186888-plasmid-map-sequence-id-366446

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  19. AAV-TRE-scFVDnmt3AL_bGHpA

    Plasmid
    #177354
    Purpose
    AAV expression of a fusion protein with scFV, catalytic domain of Dnmt3a and C terminal domain of Dnmt3l from doxycycine-inducible promoter for targeted DNA methylation editing
    Depositor
    Naoki Yamamoto
    Article
    Yamamoto et al Research Square [Preprint] 2021. doi: 10
    Insert
    catalytic domain of DNA Methyltransferase 3 Alpha (Dnmt3a Mouse)
    Use
    AAV
    Tags
    C-terminal domain of mouse Dnmt3l (194–415 aa), P…
    Expression
    Mammalian
    Mutation
    598–908 aa of mouse Dnmt3a
    Promoter
    Tetracycline-dependent promoter
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    177354-plasmid-map-sequence-id-353614

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  20. AAV-TRE-scFVDnmt3A_bGHpA

    Plasmid
    #177355
    Purpose
    AAV expression of scFV-fused catalytic domain of Dnmt3a from doxycycine-inducible promoter for targeted DNA methylation editing
    Depositor
    Naoki Yamamoto
    Article
    Yamamoto et al Research Square [Preprint] 2021. doi: 10
    Insert
    catalytic domain of DNA Methyltransferase 3 Alpha (Dnmt3a Mouse)
    Use
    AAV
    Tags
    myc and scFV
    Expression
    Mammalian
    Mutation
    598–908 aa of mouse Dnmt3a
    Promoter
    Tetracycline-dependent promoter
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    177355-plasmid-map-sequence-id-353667

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Showing: 2601 - 2620 of 5312 results