We narrowed to 9,626 results for: otos

1. pRSET-GCaMP6f

   Plasmid

   #67564

   Purpose

   bacterial expression of a fluorescent calcium reporter

   Depositor

   Ehud Isacoff

   Article

   Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

   Insert

   GCaMP6f
   

   Use

   Tags

   HIS-tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 29, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pRSetB-Peredox

   Plasmid

   #73806

   Purpose

   Expresses the Peredox fluorescent sensor protein (for NADH/NAD ratio) in bacteria, with N-terminal his-tag

   Depositor

   Gary Yellen

   Article

   Mongeon et al Antioxid Redox Signal. 2016 Mar 18.

   Insert

   Peredox
   

   Use

   Tags

   his-tag to facilitate purification

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. SPS-7942

   Plasmid

   #80459

   Purpose

   IPTG inducible expressoin of sucrose phosphate synthase derived from Synechococcus elongatus PCC 7942

   Depositor

   Daniel Ducat

   Article

   Abramson et al Plant Cell Physiol. 2016 Oct 13. pii: pc

   Insert

   Sucrose phosphate synthase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSG5-CRKL

   Plasmid

   #29560

   Depositor

   Nora Heisterkamp

   Article

   Fioretos et al Oncogene. 1993 Oct . 8(10):2853-5.

   Insert

   CRKL (CRKL Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

5. mOrange2-C1

   Plasmid

   #54650

   Purpose

   Localization: C1 Cloning Vector, Excitation: 549, Emission: 565

   Depositor

   Roger Tsien, Michael Davidson

   Article

   Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

   Type

   Empty backbone

   Use

   Tags

   mOrange2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSL3287

   Plasmid

   #139750

   Purpose

   Base Cas12a CRISPRi plasmid

   Depositor

   Himadri Pakrasi

   Article

   Knoot et al ACS Synth Biol. 2020 Jan 17;9(1):132-143

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Ttx-3-mCherry-SL2-Post-SPHERE-SF-iGluSnFR

   Plasmid

   #187100

   Purpose

   Glutamate sensor for localization to membrane contatct

   Depositor

   Kotaro Oka

   Article

   Shindo et al bioRxiv 2022

   Insert

   Post-SPHERE-SF-iGluSnFR
   

   Use

   Tags

   mCherry-SL2

   Expression

   Worm

   Mutation

   Promoter

   Ttx-3

   Available since

   Aug. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLZ12Km2-P23R:TA:fflucRT

   Plasmid

   #88901

   Purpose

   Toxin-antitoxin stabilized bioluminescent reporter plasmid for Streptococcus expression

   Depositor

   Thomas Proft

   Article

   Loh et al Appl Microbiol Biotechnol. 2013 Nov;97(2

   Insert

   toxin-antitoxin + Red-shifted firefly luciferase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CAG-RbpjEnR

   Plasmid

   #160924

   Purpose

   dominant-negative construct to inhibit Notch signaling

   Depositor

   Mark Emerson

   Article

   Chen et al Sci Rep. 2021 Jul 15;11(1):14525. doi: 1

   Insert

   RbpjEnR
   

   Use

   Tags

   Expression

   Mutation

   fused with an engrailed repressor

   Promoter

   Available since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. mRFP-RBD(R59A)-mRFP

    Plasmid

    #18664

    Depositor

    Karel Svoboda

    Article

    Yasuda et al Nat Neurosci. 2006 Feb . 9(2):283-91.

    Insert

    Ras binding domain of Raf
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    R59A mutation in RBD

    Promoter

    Available since

    July 2, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLZ12Km2-P23R:TA:ffluc

    Plasmid

    #88900

    Purpose

    Toxin-antitoxin stabilized bioluminescent reporter plasmid for Streptococcus expression

    Depositor

    Thomas Proft

    Article

    Loh et al Appl Microbiol Biotechnol. 2013 Nov;97(2

    Insert

    toxin-antitoxin + firefly luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pNH051

    Plasmid

    #42157

    Depositor

    Colin Dolphin

    Article

    Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

    Insert

    GFP-RT-GFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pQE-60NA-proinsulin-mEGFP

    Plasmid

    #160464

    Purpose

    Encodes a fusion of human proinsulin and the monomeric enhanced EGFP. This construct can be used as a negative control for superfolder activity in bacteria

    Depositor

    Benjamin Glick

    Article

    Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

    Insert

    proinsulin-mEGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. LRG2.1-mOrange

    Plasmid

    #124772

    Purpose

    3rd generation lentiviral plasmid to co-express a guide RNA and mOrange

    Depositor

    Jason Sheltzer

    Article

    Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTriEx-mVenus-Zdk1

    Plasmid

    #81047

    Purpose

    mammalian expression of Zdk1

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    Zdk1
    

    Use

    Tags

    6x His tag, FLAG, and mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA-PANX1(HCVp)

    Plasmid

    #87698

    Purpose

    Express HCV protease-activatible pannexin-1 in mammalian cells

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    PANX(HCVp)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAWP78-PVCpnf_pvc13-HA

    Plasmid

    #198296

    Purpose

    Produces PVCs retargeted with HA tag

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    PVCpnf_pvc13-HA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAWP78-PVCpnfΔpvc13

    Plasmid

    #198283

    Purpose

    Produces PVCs lacking tail fibers

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    PVCpnfΔpvc13
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAG-VSFP Butterfly 1.2

    Plasmid

    #47978

    Purpose

    Genetically encoded voltage indicator

    Depositor

    Thomas Knopfel

    Article

    Akemann et al J Neurophysiol. 2012 Oct;108(8):2323-37.

    Insert

    VSFP Butterfly 1.2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPaxillin-PSmOrange

    Plasmid

    #31923

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Methods. 2011 Jul 31;8(9):771-7. doi

    Insert

    PSmOrange
    

    Use

    Tags

    Paxillin

    Expression

    Mammalian

    Mutation

    S21T, Q63L, F100Y, L125M, K166R, P192S in PSmOran…

    Promoter

    Available since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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