165,537 results

1. PCMV-Tag4A-MMP8 (E138Q)

   Plasmid

   #29549

   Depositor

   Yardena Samuels

   Article

   Palavalli et al Nat Genet. 2009 May . 41(5):518-20.

   Insert

   Metalloproteinase protein 8 (MMP8 Human)
   

   Tags

   FLAG

   Expression

   Bacterial and Mammalian

   Mutation

   E138Q

   Available Since

   July 22, 2011

   Availability

   Academic Institutions and Nonprofits only

2. pCMV-Cre

   Plasmid

   #123133

   Purpose

   Mammalian expression vector for wild-type Cre.

   Depositor

   David Liu

   Article

   Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

   Insert

   Cre recombinase
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-Cre

   Plasmid

   #13775

   Purpose

   Mammalian expression of Cre recombinase from the broadly active CAG promoter/enhancer

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

   Insert

   Cre
   

   Use

   Cre/Lox

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 20, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pComb3XSS

   Plasmid

   #63890

   Purpose

   3rd generation plasmid for phage display on modified geneIII, contains stuffer fragment

   Depositor

   Carlos Barbas

   Article

   Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):

   Inserts

   SS Stuffer
   Light Chain Stuffer
   Heavy Chain Stuffer
   

   Use

   Phage display

   Tags

   6x His, HA tag, and geneIII

   Expression

   Bacterial

   Promoter

   lacZ

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGP-CMV-GCaMP6s

   Plasmid

   #40753

   Purpose

   Mammalian expression of ultrasensitive protein calcium sensor

   Depositor

   Douglas Kim, GENIE Project

   Article

   Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

   Insert

   GCaMP6s
   

   Tags

   6xHis, T7 epitope, and Xpress tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pXR002: EF1a-dCasRx-2A-EGFP

   Plasmid

   #109050

   Purpose

   Catalytically inactive dCasRx with 2A-EGFP

   Depositor

   Patrick Hsu

   Article

   Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

   Insert

   dCasRx
   

   Use

   CRISPR and Lentiviral

   Tags

   2A-eGFP, HA, and NLS

   Expression

   Mammalian

   Mutation

   R239A/H244A/R858A/H863A

   Available Since

   April 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEcCas

   Plasmid

   #73227

   Purpose

   Constitutive expression of cas9 and inducible expression of lambda RED and sgRNA

   Depositor

   Sheng Yang

   Article

   Li et al Acta Biochim Biophys Sin (Shanghai). 202

   Inserts

   Cas9
   sgRNA
   

   Use

   CRISPR; Red recombinase

   Expression

   Bacterial

   Promoter

   native promoter

   Available Since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. Ef1a-pspCas13b-NES-3xFLAG-T2A-BFP

   Plasmid

   #173029

   Purpose

   For expression of cytoplasmic pspCas13b protein tagged with 3xHA-T2A-BFP for gene silencing in mamallian cells.

   Depositor

   Mohamed Fareh

   Article

   Fareh et al Nat Commun. 2021 Jul 13;12(1):4270. doi:

   Insert

   pspCas13b
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG, BFP, HIV NES, and T2A

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSETB/mStayGold

   Plasmid

   #212017

   Purpose

   mStayGold has an n1 adaptor at its N-terminus and is useful for C-terminal tagging. This construct is used also for expression of mStayGold itself.

   Depositor

   Atsushi Miyawaki

   Article

   Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Insert

   mStayGold
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. hNTo1-qgRNA-pYJA5

    Plasmid

    #217781

    Purpose

    Non-targeting control 1 qgRNA-pYJA5 plasmid from the T.spiezzo library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning

    Depositor

    Adriano Aguzzi

    Article

    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038

    Insert

    Quadruple sgRNA-expression cassette for nontargeting control for gene ablation
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Expression

    Mammalian

    Promoter

    human U6, mouse U6, human H1, human 7SK

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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