We narrowed to 32,952 results for: uros

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LncRNA overexpression EF1α-BbsI-SV40polyA_puroR

Plasmid

#219826

Purpose

Overexpression vector for non-coding RNAs driven by EF1α promoter with puromycin resistance. Cloning using BbsI.

Depositor

Philipp Maass

Type

Empty backbone

Expression

Mammalian

Promoter

EF1a

Available Since

July 29, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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GG-EBNA-EEA-5guides-PGK-Puro

Plasmid

#102898

Purpose

EBNA episome plasmid for U6 promoter-driven expression of 5 gRNAs targeting EEA-motif. Includes PGK-puro selection cassette.

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

EEA-5guides-PGK-Puro

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-SARS-CoV-2_Spike_Beta-VA

Plasmid

#191218

Purpose

Lentiviral expression of SARS-CoV-2 Spike_Beta in mammalian cells

Depositor

Mohan Babu

Article

Broderick et al Nat Commun. 2025 Jul 1;16(1):5784. doi:

Insert

SPIKE_SARS2_Beta (S SARS CoV-2)

Use

Lentiviral

Tags

VA tag

Expression

Mammalian

Mutation

D80A D215G (Del 241-243) K417N E484K N501Y D614G …

Promoter

CMV

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti c-MYC-DDK-Puro GFP

Plasmid

#123299

Purpose

Mammalian expression of GFP C-MYC-FLAG

Depositor

Giovanni Tonon

Article

Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

Insert

GFP

Use

Lentiviral

Tags

MYC-FLAG

Expression

Mammalian

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pKIBra-P2A-mCherry-Puro-sfGFP

Plasmid

#115686

Purpose

Knock-in of P2A-mCherry at the mouse Brachyury (T) gene

Depositor

David Suter

Article

Deluz et al Genes Dev. 2016 Nov 15;30(22):2538-2550.

Insert

P2A-mCherry-loxP-PGK-Puro-sfGFP-loxP

Expression

Mammalian

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1-TetON-Puro-HIF1a 3809

Plasmid

#118705

Purpose

Lentivirus for expression of shRNA1 against human HIF1a (Dox-inducible)

Depositor

William Kaelin

Article

Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

Insert

shRNA against human HIF1a

Use

Lentiviral

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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HA-HIF1alpha P402A/P564A-pBabe-puro

Plasmid

#19005

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 1 alpha (HIF1A Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA changed amino acids: Proline 402 to Alanin…

Available Since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only

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Mouse 5' Npm1 AID GFP PuroR

Plasmid

#127899

Purpose

HDR (donor) Plasmid for Inserting PuroR-2A-GFP-AID into the 5' end of the Mouse Npm1 Gene

Depositor

Mark Groudine

Insert

Npm1 (Npm1 Mustard Weed, Mouse)

Use

Donor plasmid

Tags

GFP, AID, PuroR

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti CMV/TO RasV12 Puro (w119-1)

Plasmid

#22262

Depositor

Eric Campeau

Insert

HRAS G12V (HRAS Human)

Use

Lentiviral

Expression

Mammalian

Mutation

G12V mutant

Available Since

Dec. 15, 2009

Availability

Academic Institutions and Nonprofits only

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pCMV6-G1-ATP6-Myc-FLAG (puromycin)

Plasmid

#86851

Purpose

mammalian expression of ATP6 with mitochondrial targeting sequence, Myc, and FLAG tags

Depositor

Matthew O'Connor

Article

Boominathan et al Nucleic Acids Res. 2016 Nov 2;44(19):934

Insert

ATP6 (ATP6 Human)

Tags

ATP5G1 (G1) (MQTAGALFISPALIRCCTRGLIRPVSASFLNS PVN…

Expression

Mammalian

Mutation

codon corrected

Promoter

CMV

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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METTL3 shRNA 2 tet pLKO puro

Plasmid

#162984

Purpose

Tet-inducible shRNA targeting human METTL3 #2

Depositor

Mark Rubin

Article

Cotter et al Mol Cancer Res. 2021 Jun 4. pii: 1541-77

Insert

methyltransferase like 3 (METTL3 Human)

Use

Lentiviral

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1-TetON-Puro-HIF1a 0819

Plasmid

#118704

Purpose

Lentivirus for expression of shRNA1 against human HIF1a (Dox-inducible)

Depositor

William Kaelin

Article

Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

Insert

shRNA against human HIF1a

Use

Lentiviral

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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GFP shRNA tet pLKO puro

Plasmid

#163017

Purpose

Control tet-inducible shRNA targeting eGFP

Depositor

Mark Rubin

Article

Cotter et al Mol Cancer Res. 2021 Jun 4. pii: 1541-77

Insert

Enhanced Green Fluorescent Protein

Use

Lentiviral

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-Ef1a-hMGT-PGK-puro

Plasmid

#172394

Purpose

Lentiviral expression vector for human direct cardiac reprogramming

Depositor

Li Qian

Article

Garbutt et al STAR Protoc. 2020 Jun 19;1(1). doi: 10.1

Insert

Mef2c, Gata4, Tbx5 (MEF2C, GATA4, TBX5 Human)

Use

Lentiviral

Available Since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

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pFUW H2B-mEmerald-P2A-PuroR

Plasmid

#239553

Purpose

Expression and nuclear localization of mEmerald

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

mEmerald

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pLV[Exp]-Puro-CMV>{ARL13B_6gly_GFP_6Gly_R_GECO1}

Plasmid

#232880

Purpose

Expresses the ARL13B coupled to GFP and RGECO1 calcium sensor.

Depositor

Paul DeCaen

Article

DeCaen et al J Cell Physiol. 2025 Apr;240(4):e70034.

Insert

ADP-ribosylation factor-like protein 13B (ARL13B Human)

Use

Lentiviral

Tags

GFP and RGECO1

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-TRE3G-BE3RA-PGK-Puro

Plasmid

#110846

Purpose

Lentiviral vector for dox-inducible expression of BE3RA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3RA

Use

Lentiviral

Mutation

D10A

Promoter

TRE3G

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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856 pBabe puroL PTEN C124S

Plasmid

#10931

Depositor

William Sellers

Insert

PTEN (PTEN Human)

Use

Retroviral

Expression

Mammalian

Mutation

C124S

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only
TRIPZ-CTID195-GSQ-SUMO1nc-PURO

Plasmid

#208035

Purpose

Enables inducible expression of C-terminal Split-TurboID-fused SUMO1nc, to perform SUMO-ID; nc = non-cleavable mutation; selection with puromycin

Depositor

Rosa Barrio, James Sutherland

Article

Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

Insert

SUMO1nc (SUMO1 Human)

Use

Lentiviral

Tags

Myc, C-terminal Split-TurboID

Mutation

Q94P Mutation near C-terminus suppresses cleavage…

Promoter

tetO/UBC

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pSIN4-EF1a-TAL1-IRES-Puro

Plasmid

#61065

Purpose

lentiviral vector for TAL1

Depositor

Igor Slukvin

Article

Elcheva et al Nat Commun. 2014 Jul 14;5:4372. doi: 10.

Insert

TAL1 (TAL1 Human)

Use

Lentiviral

Tags

FLAG

Promoter

EF1alpha

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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