We narrowed to 31,012 results for: uros

1. pAAVS1_EF1a-MitoTrap-mCherry-IRES-Puro

   Plasmid

   #197067

   Purpose

   Donor plasmid for human AAVS1 locus knock-in, constitutive expression of MitoTrap-mCherry

   Depositor

   Julia Mahamid

   Article

   Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

   Insert

   MitoTrap-mCherry
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLentiPGK Puro DEST JNKKTR AA Clover

   Plasmid

   #90238

   Purpose

   Lentiviral vector to express JNK KTR AA (mutant) mClover under PGK promoter (With Puromycin Resistance)

   Depositor

   Markus Covert

   Article

   Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

   Insert

   JNK Kinase Translocation Reporter (AA mutant)
   

   Use

   Lentiviral

   Tags

   mClover

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   March 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMRX-IRES-puro-3xFLAG-NBR1

   Plasmid

   #204546

   Purpose

   Expresses 3xFLAG-tagged NBR1 in mammalian cells

   Depositor

   Masaaki Komatsu

   Article

   Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

   Insert

   NBR1 (NBR1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Aug. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti CMV Puro DHFR.dn-cHSF1

   Plasmid

   #134729

   Purpose

   Lentiviral expression vector for constitutively active dominant-negative HSF1 fused to DHFR destabilized domain

   Depositor

   Matthew D. Shoulders

   Article

   Moore et al ACS Chem Biol. 2016 Jan 15;11(1):200-10.

   Insert

   DHFR.dn-cHSF1 (HSF1 Human)
   

   Use

   Lentiviral

   Tags

   DHFR

   Expression

   Mammalian

   Mutation

   Deletion of amino acids 186-202, 379−529

   Promoter

   CMV

   Available since

   Aug. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBABE-2XFLAG,2XHA-CyclinF-puro

   Plasmid

   #32976

   Depositor

   Michele Pagano

   Article

   D'Angiolella et al Nature. 2010 Jul 1;466(7302):138-42.

   Insert

   CYCLIN F (CCNF Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   5'LTR

   Available since

   Feb. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pLKO-Tet-puro-hRAF1-shRNA-1

   Plasmid

   #185371

   Purpose

   For tetracycline-inducible mammalian expression of shRNA: CATGAGTATTTAGAGGAAGTA that targets human RAF1

   Depositor

   Ayaz Najafov

   Insert

   RAF1 (RAF1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   June 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAVS1_Puro_hPGK1_hGEM(1-110)_mNG_hSLBP(18-108)_FS

   Plasmid

   #191527

   Purpose

   Donor for targeted integration of 3xFLAG-2xSTREP tagged S-phase specific probe (FUCCI-S) to the AAVS1 safe harbor locus

   Depositor

   Yannick Doyon

   Insert

   hGEM(1-110)_mNG_hSLBP(18-108)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   mNeonGreen (mNG) + 3xFLAG_TwinStrep

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Pb459-mU6-sgRNA-EF1a-PuroR

   Plasmid

   #195507

   Purpose

   piggybac vector expressing non-targeting control sgRNA cloned using BlpI and BstXI sites

   Depositor

   Joanna Wysocka

   Article

   Bajpai et al Science. 2023 Aug 11;381(6658):eade6289.

   Insert

   Puromycin
   

   Use

   CRISPR; Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. M78 pIRESpuro-CBP-2xTEV-StrepTag

   Plasmid

   #17132

   Depositor

   Randall Moon

   Article

   Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

   Type

   Empty backbone

   Use

   Tags

   2xTEV, CBP, and Streptavidin

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMSCV-FLAG-2xSTREP-CCND3-Puro

    Plasmid

    #172619

    Purpose

    Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D3 and a puromycin resistance marker in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND3 (CCND3 Human)
    

    Use

    Retroviral

    Tags

    FLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lv EF1a HP1cs-Frb1x PGK Puro

    Plasmid

    #102808

    Purpose

    Lentiviral plasmid from FIRE-Cas9 system to express HP1cs-Frb1x fusion protein

    Depositor

    Jerry Crabtree

    Article

    Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

    Insert

    HP1cs-Frb1x
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLEX-FLAG-NRASQ61K-IRES-Puro

    Plasmid

    #120575

    Purpose

    Expresses Flag-N-Ras Q61K fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    NRAS (NRAS Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Q61K

    Promoter

    CMV

    Available since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. ePB-PURO-FLAG-METTL3-APPA

    Plasmid

    #160253

    Purpose

    For stable integration of catalytic inactive FLAG-METTL3 in human cells with trasposase

    Depositor

    Alessandro Fatica

    Article

    Sorci et al Cell Death Dis. 2018 Jul 23;9(8):796. do

    Insert

    methyltransferase like 3 (METTL3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changes in aa 395–398, DPPW → APPA

    Promoter

    tight TRE promoter

    Available since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-CMVSP6-NR2F2-SV40-PURO

    Plasmid

    #138361

    Purpose

    Lentiviral plasmid contains human NR2F2 driven by CMVSP6 and puromycin resistance for selection

    Depositor

    Nathan Lawson

    Article

    Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

    Insert

    Human NR2F2, with puromycin driven by SV40 for selection
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. 911 pBabe puroL PTEN G129E

    Plasmid

    #10771

    Depositor

    William Sellers

    Insert

    PTEN (PTEN Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    G129E

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

16. RRL.sin.cPPT.SFFV/Flag-E2-crimson.IRES-puro.WPRE (CG568)

    Plasmid

    #139445

    Purpose

    Lentiviral vector to ectopically express Flag-tagged E2-crimson from SFFV promoter

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    FLAG-tag E2-crimson
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDestTol2CG2-Neurog1-Magneto2.0-p2A-mCherry-pA

    Plasmid

    #74302

    Purpose

    Expression of Magneto2.0-p2A-mCherry in Rohon-Beard sensory neurons of the zebrafish

    Depositor

    Ali Guler

    Article

    Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

    Insert

    Magneto2.0 (FTL, FTH1, Trpv4 Human, Rat)
    

    Use

    Zebrafish

    Tags

    FLAG, cmcl2::EGFP, and p2A-mCherry

    Expression

    Mutation

    TRPV4: delta 760-871

    Promoter

    Neurog1

    Available since

    May 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUC-DEST-hSOX2-EGFP-PuroTK

    Plasmid

    #141026

    Purpose

    Reporter integration

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. shPBRM1#131-Tet-pLKO-puro

    Plasmid

    #107400

    Purpose

    Lentiviral expression of shRNA targeting PBRM

    Depositor

    William Kaelin

    Article

    Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

    Insert

    shRNA targeting PBRM1 (PBRM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKLV2-U6gRNA5(hWWTR1-W1K)-PGKpuro2AmCherry-W

    Plasmid

    #163176

    Purpose

    Lentiviral gRNA plasmid targeting human WWTR1 gene, co-expression of mCherry tag

    Depositor

    Sok Ching Cheong

    Article

    Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

    Insert

    WWTR1 (WWTR1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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