We narrowed to 1,459 results for: Mbp

1. poly E67R SUMO1

   Plasmid

   #221072

   Purpose

   Expression of poly E67R SUMO1 protein under T7 promoter

   Depositor

   Michael Rosen

   Article

   Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

   Insert

   Five repeats of E67R SUMO1 each separated by (GGS)4 (SUMO1 Human)
   

   Use

   Tags

   His and MBP

   Expression

   Bacterial

   Mutation

   E67R

   Promoter

   tac

   Available since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. poly H35K SUMO1

   Plasmid

   #221070

   Purpose

   Expression of poly H35K SUMO1 protein under T7 promoter

   Depositor

   Michael Rosen

   Article

   Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

   Insert

   Five repeats of H35K SUMO1 each separated by (GGS)4 (SUMO1 Human)
   

   Use

   Tags

   His and MBP

   Expression

   Bacterial

   Mutation

   H35K

   Promoter

   tac

   Available since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. poly H35R SUMO1

   Plasmid

   #221069

   Purpose

   Expression of poly H35R SUMO1 protein under T7 promoter

   Depositor

   Michael Rosen

   Article

   Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

   Insert

   Five repeats of H35R SUMO1 each separated by (GGS)4 (SUMO1 Human)
   

   Use

   Tags

   His and MBP

   Expression

   Bacterial

   Mutation

   H35R

   Promoter

   tac

   Available since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJCC_162 SpCas9 KES(1107-1109)GG

   Plasmid

   #179526

   Purpose

   For bacterial expression of SpCas9 KES(1107-1109)GG (phosphate lock loop mutant) with an N-terminal His-MBP tag

   Depositor

   Jennifer Doudna

   Article

   Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

   Insert

   SpCas9 KES(1107-1109)GG
   

   Use

   Tags

   10X His, TEV protease cleavage site, and maltose-…

   Expression

   Bacterial

   Mutation

   replaced KES (residues 1107-1109) with GG

   Promoter

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJCC_163 SpCas9 K233A/K234A/K253A/K263A

   Plasmid

   #179527

   Purpose

   For bacterial expression of SpCas9 K233A/K234A/K253A/K263A (helix-rolling basic patch mutant) with an N-terminal His-MBP tag

   Depositor

   Jennifer Doudna

   Article

   Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

   Insert

   SpCas9 K233A/K234A/K253A/K263A
   

   Use

   Tags

   10X His, TEV protease cleavage site, and maltose-…

   Expression

   Bacterial

   Mutation

   changed lysines 233, 234, 253, and 263 to alanine

   Promoter

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. EcRfaHKOW-L1L2NusG

   Plasmid

   #113126

   Purpose

   Expresses C-terminus of E. coli RfaH with L1 and L2 loops replaced with NusG residues

   Depositor

   James Berger

   Article

   Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

   Insert

   RfaH (rfaH Escherichia coli)
   

   Use

   Tags

   His6-MBP-N10-TEV

   Expression

   Bacterial

   Mutation

   Deleted residues 1-107, replaced L1 and L2 loops …

   Promoter

   T7

   Available since

   Oct. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMTTH+mEGFP-H1.4ΔCTD

   Plasmid

   #157800

   Purpose

   Expression of mEGFP fused to human linker histone H1.4 without c-terminal tail

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   mEGFP-H1.4ΔCTD (H1-4 Human)
   

   Use

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   pTAC

   Available since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMTTH+LANA-H1.4CTD

   Plasmid

   #157798

   Purpose

   Expression of LANA peptide-human linker histone H1.4 CTD fusion protein

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   LANA-H1.4CTD (H1-4 Human)
   

   Use

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   pTAC

   Available since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHTSHP-ShhN-Cys

   Plasmid

   #121136

   Purpose

   Expresses ShhN with a C-terminal cysteine residues in bacteria

   Depositor

   Philip Beachy

   Article

   Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

   Insert

   shh (Shh Mouse)
   

   Use

   Tags

   HIS, HRV3C site, and MBP

   Expression

   Bacterial

   Mutation

   Amino acid sequence from 26-191, with an addition…

   Promoter

   tac

   Available since

   March 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMAL c2x Tral N

    Plasmid

    #113009

    Purpose

    For bacterial expression of MBP fusion of N terminal region of Drosophila Tral

    Depositor

    Terry Orr-Weaver

    Article

    Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

    Insert

    N terminus of tral (tral Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    amino acids 1-355

    Promoter

    Available since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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