We narrowed to 31,550 results for: Eng;

1. pJH4687

   Plasmid

   #200783

   Purpose

   Pnpr-4 ins-22::pHluorin unc-54 3' UTR C.elegans AVA and other neurons expression of ins-22 pHluorin

   Depositor

   Mei Zhen

   Article

   Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

   Insert

   ins-22 (ins-22 Nematode)
   

   Use

   Tags

   pHiuorin

   Expression

   Worm

   Mutation

   Promoter

   Pnpr-4

   Available since

   May 7, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pcDNA3-HCMVgH (Merlin)

   Plasmid

   #136448

   Purpose

   Mammalian expression plasmid for HCMV glycoprotein H (Merlin strain), full-length

   Depositor

   Erik Procko

   Article

   Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

   Insert

   glycoprotein H (UL75 Human betaherpesvirus 5)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Codon-optimized for human cell expression.

   Promoter

   CMV

   Available since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVC-Ds-ubb:MCP-p65-HSF1-2a-Cerulean-Ds

   Plasmid

   #119074

   Purpose

   Zebrafish ubiquitin promoter driving MCP-p65-HSF1 with Cerulean reporter

   Depositor

   Tatjana Sauka-Spengler

   Article

   Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

   Insert

   ubb:MCP-p65-HSF1-2a-Cerulean
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Zebrafish ubiquitin promoter

   Available since

   Feb. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pVC-Ds-ubb:Com-KRAB-2a-Cerulean-Ds

   Plasmid

   #119075

   Purpose

   Zebrafish ubiquitin promoter driving Com-KRAB with Cerulean reporter

   Depositor

   Tatjana Sauka-Spengler

   Article

   Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

   Insert

   ubb:Com-KRAB-2a-Cerulean
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Zebrafish ubiquitin promoter

   Available since

   Feb. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pVC-Ds-DrU6a:sgRNA-com-Ds

   Plasmid

   #119077

   Purpose

   To clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x com stem loops

   Depositor

   Tatjana Sauka-Spengler

   Article

   Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

   Insert

   U6a:2xCom-sgRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Zebrafish U6a promoter

   Available since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pVC-Ds-DrU6a:sgRNA-MS2-Ds

   Plasmid

   #119076

   Purpose

   To clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x MS2 stem loops

   Depositor

   Tatjana Sauka-Spengler

   Article

   Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

   Insert

   U6a:2xMS2-sgRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Zebrafish U6a promoter

   Available since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. EE-mut SV40 luc

   Plasmid

   #8460

   Depositor

   Bob Weinberg

   Article

   Geng et al Oncogene 1996 Mar 21;12(6):1173-80.

   Insert

   human cyclin E promoter fragment, mutant (CCNE1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   E2F sites I, II, and III mutated.

   Promoter

   Available since

   May 24, 2006

   Availability

   Academic Institutions and Nonprofits only

8. pRKB134_GI-DUX4-intron2(-)

   Plasmid

   #83968

   Purpose

   to test DUX4 mRNA is subjected to NMD upon intron2 inclusion

   Depositor

   Robert Bradley

   Article

   Feng et al Elife. 2015 Jan 7;4. doi: 10.7554/eLife.

   Insert

   DUX4-3'UTR-intron2(-) (DUX4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouseCMV

   Available since

   Jan. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

9. pRKB133_GI-DUX4-intron2(+)

   Plasmid

   #83967

   Purpose

   to test DUX4 mRNA is subjected to NMD upon intron2 inclusion

   Depositor

   Robert Bradley

   Article

   Feng et al Elife. 2015 Jan 7;4. doi: 10.7554/eLife.

   Insert

   DUX4-3'UTR-intron2(+) (DUX4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouseCMV

   Available since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pSMOX-mNG::LacI-V3

    Plasmid

    #210224

    Purpose

    Integrates a T7 RNA polymerase, tetracycline repressor and an mNeongreen Lac repressor fusion protein with a puromycin drug selection marker into the tubulin locus.

    Depositor

    Calvin Tiengwe

    Article

    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

    Insert

    mNeongreen Lac repressor fusion protein
    

    Use

    Trypanosoma brucei expression

    Tags

    Expression

    Mutation

    Promoter

    Pol II readthrough transcription after integration

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pILGFP97

    Plasmid

    #83551

    Purpose

    Used to evaluate the expression output of CYC1 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    CYC1 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    CYC1

    Available since

    Dec. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPMVAugw

    Plasmid

    #98300

    Purpose

    Enhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of CUP1 promoter.

    Depositor

    Claudia Vickers

    Article

    Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

    Insert

    TEFM1EfmvaE>TEBS1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-3xHAtag-SP-PTPRO

    Plasmid

    #191696

    Purpose

    For expression coding sequencing of PTPRO

    Depositor

    Yuejun Chen

    Article

    Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

    Insert

    signal peptide, 3xHAtag and full length coding sequence of PTPRO
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. tdEos-VSVG-16

    Plasmid

    #57693

    Purpose

    Localization: Golgi/ER Transport, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

    Insert

    VSVG
    

    Use

    Tags

    tdEos

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pLV-EF1a-V5-Sec61B

    Plasmid

    #120244

    Purpose

    Expresses V5-tagged Sec61B in lentiviral vector

    Depositor

    Larry Gerace

    Article

    Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

    Insert

    Sec61B (Sec61b Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. RCAS (A) Wnt3a (CT#169)

    Plasmid

    #13976

    Depositor

    Cliff Tabin

    Article

    Kengaku et al Science. 1998 May 22. 280(5367):1274-7.

    Insert

    Wnt3a (Wnt3a Mouse)
    

    Use

    Retroviral; Avian expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pCMV-Nxph4-HA

    Plasmid

    #128102

    Purpose

    Express HA tagged Nxph4

    Depositor

    Huda Zoghbi

    Article

    Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

    Insert

    Nxph4 (Nxph4 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 2-Luc-Del-50

    Plasmid

    #21730

    Depositor

    Wafik El-Deiry

    Article

    Zeng et al Nat Genet. 1997 Jan . 15(1):78-82.

    Insert

    p21 promoter deletion mutant (CDKN1A Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Identical to 2-Luc except that the 50-bp region b…

    Promoter

    Available since

    Aug. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pCAG-SP-CLSTN2-3xHAtag

    Plasmid

    #191693

    Purpose

    For expression coding sequencing of CLSTN2

    Depositor

    Yuejun Chen

    Article

    Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

    Insert

    signal peptide, full length coding sequence of CLSTN2 and 3xHAtag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p3×FLAG-CMV-14-LanCL2

    Plasmid

    #73391

    Purpose

    Expresses FLAG tagged LanCL2

    Depositor

    Jie Chen

    Article

    Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

    Insert

    LanCL2 (LANCL2 Human)
    

    Use

    Tags

    3x FLAG tagged

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. MCV-Rep-

    Plasmid

    #32058

    Depositor

    Patrick Moore

    Article

    Feng et al PLoS One. 2011;6(7):e22468. Epub 2011 Ju

    Insert

    MCV-Rep- genome
    

    Use

    Tags

    Expression

    Mutation

    Single cytosine to adenine (C/A) mutation at 2,07…

    Promoter

    none from vector. Insert contains native promoter…

    Available since

    Oct. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTYB3-human PUM1-HD MUT6-2 7-2 (N1043S-Q1047E-S1079N-E1083Q)-intein-CBD

    Plasmid

    #73300

    Purpose

    Expresses mutant human PUM1-HD

    Depositor

    Traci Hall

    Article

    Cheong et al Proc Natl Acad Sci U S A. 2006 Sep 12;10

    Insert

    human PUM1 Pumilio homology domain (PUM1 Human)
    

    Use

    Tags

    intein

    Expression

    Bacterial

    Mutation

    N1043S-Q1047E-S1079N-E1083Q

    Promoter

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

23. pPIGA-PHD

    Plasmid

    #26778

    Depositor

    Linzhao Cheng

    Article

    Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

    Insert

    PGK-Hyg-p(A) (PGK1 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Hiv7CMV-Cremyc-2A-GFP

    Plasmid

    #117148

    Purpose

    Express Cre enzyme in mammalian cells with floxed genes to achieve somatic knockout. GFP is simultaneously expressed for convenient tracking the transfected cells.

    Depositor

    Qiang Lu

    Article

    Geng et al Nat Commun. 2018 Jul 13;9(1):2707. doi:

    Insert

    Cremyc-2A-GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pHCas9-Nours

    Plasmid

    #107733

    Purpose

    Escherichia coli- S. cerevisiae shuttle plasmid harbors a Cas9 gene, a natMX6 and URA3 selection markers used in S. cerevisiae

    Depositor

    Sheng Yang

    Article

    Wang et al Biotechnol Bioeng. 2018 Sep 10. doi: 10.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Human optimized

    Promoter

    TEF1

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET-30a(+)-Syk-tSH2

    Plasmid

    #111269

    Purpose

    express murine Syk tandem SH2 domains (Ser 8 to Gln 264), the unphosphorylated form, in E.coli strain Rosetta 2 (DE3)

    Depositor

    Carol Post

    Article

    Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5

    Insert

    murine Syk tandem SH2 domains (Ser 8 to Gln 264), the unphosphorylated form (Syk Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJKW1813

    Plasmid

    #174003

    Purpose

    Expresses PpCYP90G4 and AtATR1 for reconstitution of diosgenin biosynthesis in yeast

    Depositor

    Jing-Ke Weng

    Article

    Christ et al Nat Commun. 2019 Jul 19;10(1):3206. doi:

    Inserts

    PpCYP90G4
    ATR1 (ATR1 Mustard Weed)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCMV-Nxph4-6A-HA

    Plasmid

    #128103

    Purpose

    Express HA tagged Nxph4-6A

    Depositor

    Huda Zoghbi

    Article

    Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

    Insert

    Nxph4 (Nxph4 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. FUP95PDZGW (C5)

    Plasmid

    #74021

    Purpose

    lentiviral expression of Psd95-EGFP fusion

    Depositor

    Oliver Schluter, Robert Malenka, Weifeng Xu

    Article

    Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

    Insert

    Psd95 (DLG4 Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    PDZ

    Promoter

    hUbC

    Available since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

30. pITGFP89

    Plasmid

    #83560

    Purpose

    Used to evaluate the expression output of TEF1 promoter with yEGFP-CLN2PEST (destabilized GFP) used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    TEF1 promoter -yEGFP-CLN2PEST
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. tetO7_Ef1a_OCT4

    Plasmid

    #18918

    Depositor

    Linzhao Cheng

    Article

    Mali et al Stem Cells. 2008 Aug . 26(8):1998-2005.

    Insert

    OCT4 (POU5F1 Human)
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 4, 2008

    Availability

    Academic Institutions and Nonprofits only

32. L1-1147

    Plasmid

    #13267

    Depositor

    Vance Lemmon

    Article

    Cheng et al J Neurosci. 2005 Jan 12. 25(2):395-403.

    Insert

    L1CAM (L1CAM Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Truncated in the juxtamembrane region

    Promoter

    Available since

    Dec. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

33. pVMG0302_Cq-U6-1_2xBbsI_gRNA-Loop

    Plasmid

    #169369

    Purpose

    Expression of gRNA in Culex quinquefasciatus or other mosquitoes

    Depositor

    Valentino Gantz

    Article

    Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

    Insert

    pVMG0302_Cq-U6-1_2xBbsI_gRNA-Loop
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    CPIJ039653

    Available since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLV-EF1a-V5-Vrk2

    Plasmid

    #120240

    Purpose

    Expresses V5-tagged Vrk2 in lentiviral vector

    Depositor

    Larry Gerace

    Article

    Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

    Insert

    Vrk2 (Vrk2 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pVMG0146_Cq-U6-1_2xBbsI-gRNA

    Plasmid

    #169238

    Purpose

    Expression of gRNA in Culex quinquefasciatus or other mosquitoes

    Depositor

    Valentino Gantz

    Article

    Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

    Insert

    Cq-U6-1_2xBbsI-gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    CPIJ039653

    Available since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pVMG0149_Cq-U6-6_2xBbsI-gRNA

    Plasmid

    #169323

    Purpose

    Expression of gRNA in Culex quinquefasciatus or other mosquitoes

    Depositor

    Valentino Gantz

    Article

    Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

    Insert

    pVMG0149_Cq-U6-6_2xBbsI-gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    CPIJ039596

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3.1 Flag-Sesn1b

    Plasmid

    #61867

    Purpose

    Transient overexpression of Flag-Sesn1b (mouse)

    Depositor

    Ming Li

    Article

    Peng et al Cell. 2014 Sep 25;159(1):122-33. doi: 10

    Insert

    Sesn1 (Sesn1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

38. pGEM-myr-eGFP-SV40pA-FKF

    Plasmid

    #119866

    Purpose

    Donor cassette containing myr-tagged eGFP and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

    Depositor

    Tatjana Sauka-Spengler

    Article

    Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d

    Insert

    myr-tagged eGFP and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    

    Use

    Tags

    2x myristoylation

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. p3xFLAG-CMV-zDicer

    Plasmid

    #171539

    Purpose

    Encodes Zebrafish Dicer protein

    Depositor

    Yan Zeng

    Article

    Li et al Biochem Biophys Res Commun. 2019 Jun 18;

    Insert

    dicer1(MK642699) (dicer1 Zebrafish)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pGEM-myr-tdTomato-SV40pA-FKF

    Plasmid

    #119867

    Purpose

    Donor cassette containing myr-tagged tdTomato and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

    Depositor

    Tatjana Sauka-Spengler

    Article

    Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d

    Insert

    myr-tagged tdTomato and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    

    Use

    Tags

    2x myristoylation

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge