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bRA77

Plasmid

#100953

Purpose

GAL1 driven Cas9. gRNA can be cloned into BplI sites.

Depositor

James Haber

Article

Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV Aquamarine

Plasmid

#157777

Purpose

Expresses cyan fluorescent Aquamarine in cells

Depositor

Marc Tramier

Article

Bertolin et al ACS Sens. 2019 Aug 23;4(8):2018-2027. do

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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Double UP mNeon to mScarlet FRT

Plasmid

#141111

Purpose

Natively produces mNeonGreen, in the presence of Flp or Flpe will be recombined to produce mScarlet.

Depositor

Erik Dent

Article

Taylor et al Front Mol Neurosci. 2020 May 20;13:82. d

Type

Empty backbone

Expression

Mammalian

Promoter

CAG

Available Since

July 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_COX8-Halo-PanK

Plasmid

#197949

Purpose

Expression of FRET_control in mammalian cells

Depositor

Kai Johnsson

Article

Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

Insert

Halo-ecPanK

Expression

Mammalian

Mutation

PanK=PanK-D127A,L277W,F252Y

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pIVT2

Plasmid

#122140

Purpose

For making cRNA appropriate for microinjection

Depositor

Carmen Williams

Article

Winuthayanon et al Elife. 2015 Dec 1;4:e10453. doi: 10.7554

Type

Empty backbone

Use

For in vitro transcription

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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UAS-myr::4xSNAPf

Plasmid

#87637

Purpose

For creating drosophila transgenics expressing UAS-myr4x::SNAPf

Depositor

Gregory Jefferis

Article

Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

Insert

myr::4xSNAPf

Expression

Insect

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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pBAD/HisD-LSSmKate2

Plasmid

#31909

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

LSSmKate2

Tags

HIS

Expression

Bacterial

Mutation

K69Y/P131T/S148G/M167D/T183S/M196V compared to mK…

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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JP1520 PIK3CA WT HA

Plasmid

#14570

Depositor

Joan Brugge

Article

Isakoff et al Cancer Res. 2005 Dec 1. 65(23):10992-100

Insert

PIK3CA (PIK3CA )

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Available Since

April 12, 2007

Availability

Academic Institutions and Nonprofits only

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VV001: WT Arch-3 in pET28b

Plasmid

#58487

Purpose

Expresses WT Archaerhodopsin-3 (with 6x C-terminal His tag) in E. coli

Depositor

Adam Cohen

Article

Maclaurin et al Proc Natl Acad Sci U S A. 2013 Apr 9;110

Insert

Archaerhodopsin-3 (with C-terminal His tag)

Expression

Bacterial

Promoter

T7

Available Since

July 23, 2014

Availability

Academic Institutions and Nonprofits only

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pBAD/HisD-LSSmKate1

Plasmid

#31908

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

LSSmKate1

Tags

HIS

Expression

Bacterial

Mutation

K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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pCOLA-Spec-EV

Plasmid

#212095

Purpose

(Empty Backbone) pCOLA-Spec Empty Vector

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pGBHPS-SortA

Plasmid

#21930

Depositor

Fuyuhiko Inagaki

Article

Kobashigawa et al J Biomol NMR. 2009 Mar . 43(3):145-50.

Insert

sortase

Tags

GB1, HRV3C cleagage site, and His

Expression

Bacterial

Mutation

deleted amino acids 1-59

Available Since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only

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beta2 (px330:2-guideCas9)

Plasmid

#169435

Purpose

Expression of Cas9 and 2 guides

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

spCas9

Use

CRISPR

Expression

Mammalian

Available Since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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OmpT

Plasmid

#213137

Purpose

OmpT_native membrane expression

Depositor

Sebastian Hiller

Article

Rath et al Nat Commun. 2023 Sep 13;14(1):5648. doi:

Insert

OmpT

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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UAS-Halo7::CAAX

Plasmid

#87645

Purpose

For creating drosophila transgenics expressing UAS-Halo7::CAAX

Depositor

Gregory Jefferis

Article

Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

Insert

Halo7::CAAX

Expression

Insect

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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HA-mULK1M92A

Plasmid

#31964

Depositor

Do-Hyung Kim

Article

Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

Insert

ULK1 (Ulk1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

M92A

Promoter

CMV, SP6

Available Since

Oct. 31, 2011

Availability

Academic Institutions and Nonprofits only
pET28a-clbC

Plasmid

#114145

Purpose

Expresses ClbC-nHis

Depositor

Emily Balskus

Article

Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

Insert

clbC

Tags

6xHis-thrombin-T7 tag

Expression

Bacterial

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pET29b-clbBpks

Plasmid

#114154

Purpose

Expresses the PKS module of ClbB

Depositor

Emily Balskus

Article

Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

Insert

clbB

Tags

6xHis

Expression

Bacterial

Mutation

Only contains the PKS module of ClbB

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-clbH-A1

Plasmid

#114149

Purpose

Expresses ClbH-A1

Depositor

Emily Balskus

Article

Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

Insert

clbH-A1

Tags

6xHis-thrombin-T7 tag

Expression

Bacterial

Mutation

Contains only the A1 domain of ClbH

Available Since

Aug. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pKI1.1

Plasmid

#85807

Purpose

CRISPR/Cas9 for Arabidopsis in pENTR/D-TOPO

Depositor

Tetsuya Higashiyama

Article

Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

Insert

human-codon-optimized SpCas9

Use

CRISPR

Tags

FLAG and NLS

Promoter

AtRPS5A

Available Since

May 18, 2017

Availability

Academic Institutions and Nonprofits only

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