We narrowed to 6,573 results for: tac
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Plasmid#235276PurposeComMAND open-loop circuit regulating mRuby2 with miR-FF5DepositorInsertmRuby2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1a (human EF1a)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-mRuby2(miRE.FF4)-TS.FF6x1-bGH
Plasmid#235270PurposeComMAND open-loop circuit regulating mRuby2, EF1aDepositorInsertmRuby2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1a (human EF1a)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-mRuby2(miRE.FF4)-TS.FF4x1-bGH
Plasmid#235271PurposeComMAND closed-loop circuit regulating mRuby2, EF1aDepositorInsertmRuby2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1a (human EF1a)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-mRuby2(miR.FF4)-TS.FF4x1-bGH
Plasmid#235283PurposeComMAND closed-loop circuit regulating mRuby2 with miR-FF4DepositorInsertmRuby2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1a (human EF1a)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-TS.FF4x1-mRuby2-bGH
Plasmid#235274Purpose3' microRNA target site expression for dual-vector iFFL circuit (closed-loop)DepositorInsertmRuby2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1a (human EF1a)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-mRuby2(miRE.FF5)-TS.FF5x1-bGH
Plasmid#235261PurposeComMAND closed-loop circuit regulating mRuby2 with miRE-FF5DepositorInsertmRuby2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1a (human EF1a)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-tagBFP-bGH
Plasmid#235257PurposetagBFP expression to serve as a "filler" plasmid for transfectionsDepositorInserttagBFP
UseSynthetic BiologyTagsExpressionMammalianMutationSilent mutations to remove BsaI sites in tagBFPPromoterEF1a (human EF1a)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
EFS-mRuby2-bGH
Plasmid#235253PurposeComMAND base gene regulating mRuby2, EFS-drivenDepositorInsertmRuby2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEFS (human EF1a short, intronless)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-mRuby2-bGH
Plasmid#235269PurposeComMAND base gene regulating mRuby2, EF1aDepositorInsertmRuby2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1a (human EF1a)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-TS.FF6x1-mRuby2-bGH
Plasmid#235275Purpose3' microRNA target site expression for dual-vector iFFL circuit (open-loop)DepositorInsertmRuby2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1a (human EF1a)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-mRuby2-TS.FF4x1-bGH
Plasmid#235272Purpose3' microRNA target site expression for dual-vector iFFL circuit (closed-loop)DepositorInsertmRuby2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1a (human EF1a)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-mRuby2-TS.FF6x1-bGH
Plasmid#235273Purpose3' microRNA target site expression for dual-vector iFFL circuit (open-loop)DepositorInsertmRuby2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1a (human EF1a)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
FUGW U6 gL1HSg2dCas9-KRAB-T2a-GFP
Plasmid#234881PurposeL1HS-silencing plasmid (CRISPRi gRNA2)DepositorInsertLacZ gRNA
UseCRISPR and LentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceApril 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUC57-ITR2-H1- sgCTG Frodo-eCMV-SaSpD10A (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2
Plasmid#210734PurposeCoding for SaSp D10A Cas9 alongside Frodo sgRNA targeting CAG repeatsDepositorInsertsSaSp D10A Cas9
Frodo sgCAG
UseCRISPRTags3xFLAG, SV40 NLS, PolyA signalExpressionMammalianMutationD10A, N-terminal Sa Cas9, C-terminal Sp Cas9PromoterH1 and eCMVAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
Kif3A-EGFP-SNAP
Plasmid#229852PurposeKif3A (aa1-342)-Kif1A neck linker(17aa) fused to Kin1 coiled coil (aa345-406)-EGFP-SNAP-his. This Kif3A was used to link an oligo to the motor using the SNAP tag for single molecule experimentsDepositorInsertKif3A (Kif3a Mouse)
UseTags6xHis, EGFP, and SNAP-tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
LVDP 2P.2
Plasmid#231904PurposeFor the production of SARS-CoV-2 virus-like particles (VLPs) in the '2-plasmid' system and packaging firefly luciferase mRNA into the VLPsDepositorUseLentiviralTagsExpressionMutationR203M in N proteinPromoterCMV, EF-1aAvailable sinceJan. 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2 spike G12345 mutant
Plasmid#231920PurposeFor expression of SARS-CoV-2 spike protein with glycan deletion at N1098, N1134, N1158, N1173, N1194 by implementing Asn-to-Gln mutationDepositorInsertspike G12345 (S SARS-CoV-2)
UseTagsFLAGExpressionMammalianMutationN1098Q, N1134Q, N1158Q, N1173Q, N1194QPromoterCMVAvailable sinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
LVDP 2P.2.EGFP
Plasmid#231905PurposeFor the production of SARS-CoV-2 virus-like particles (VLPs) in the '2-plasmid' system and packaging EGFP mRNA into the VLPsDepositorUseLentiviralTagsExpressionMutationR203M in N proteinPromoterCMV, EF-1aAvailable sinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2 spike G13 mutant
Plasmid#231915PurposeFor expression of SARS-CoV-2 spike protein with glycan deletion at N1098, N1158 by implementing Asn-to-Gln mutationDepositorInsertspike G13 (S SARS-CoV-2)
UseTagsFLAGExpressionMammalianMutationN1098Q, N1158QPromoterCMVAvailable sinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2 spike G14 mutant
Plasmid#231916PurposeFor expression of SARS-CoV-2 spike protein with glycan deletion at N1098, N1173 by implementing Asn-to-Gln mutationDepositorInsertspike G14 (S SARS-CoV-2)
UseTagsFLAGExpressionMammalianMutationN1098Q, N1173QPromoterCMVAvailable sinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only