4,181 results

1. ATeam1.03-nD/nA/pcDNA3

   Plasmid

   #51958

   Purpose

   mammalian expression of ATeam1.03-nD/nA, a FRET-based ATP indicator

   Depositor

   Takeharu Nagai

   Article

   Kotera et al ACS Chem Biol. 2010 Feb 19;5(2):215-22.

   Insert

   ATeam1.03-nD/nA
   

   Expression

   Mammalian

   Available Since

   March 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE TRE dCas9-KRAB

   Plasmid

   #50917

   Purpose

   Tet-regulatable dCas9-KRAB 2nd generation lentiviral expression vector

   Depositor

   Scot Wolfe, Rene Maehr

   Article

   Kearns et al Development. 2014 Jan;141(1):219-23. doi

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and KRAB

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically inactive)

   Promoter

   TRE

   Available Since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. EGFR L858R

   Plasmid

   #11012

   Purpose

   Retroviral construct for expressing human EGFR with L858R mutation in human cells

   Depositor

   Matthew Meyerson

   Article

   Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

   Insert

   EGFR L858R (EGFR Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   L858R (tumor-derived)

   Available Since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVUT-tTR-KRAB

   Plasmid

   #11651

   Purpose

   Tet-regulated (Tet-on) lentiviral vector for transgene (hUbiquitin promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 3rd generation

   Depositor

   Patrick Aebischer, Didier Trono

   Article

   Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

   Has Service

   Cloning Grade DNA

   Insert

   hUbiquitin C, GFP, tTR-KRAB, Tet-on
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSLQ9926: pHR-PGK-SV40_NLS-dCasMINI-V4-VPR-c- Myc_NLS-mCherry-WPRE

   Plasmid

   #176269

   Purpose

   Expression of dCasMINI-V4-VPR-mCherry in mammalian cells

   Depositor

   Stanley Qi

   Article

   Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

   Insert

   dCasMINI-V4-VPR-mCherry
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti CMV Hygro DEST (w117-1)

   Plasmid

   #17454

   Purpose

   3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Hygro

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Type

   Empty backbone

   Use

   Lentiviral; Destination vector

   Expression

   Mammalian

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCW57-MCS1-P2A-MCS2 (GFP)

   Plasmid

   #80924

   Purpose

   All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

   Depositor

   Adam Karpf

   Article

   Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

   Type

   Empty backbone

   Use

   Lentiviral; Doxycycline inducible; p2a self cleav…

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Aug. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-BRD2

   Plasmid

   #65376

   Purpose

   GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

   Depositor

   Kyle Miller

   Article

   Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

   Insert

   BRD2 (BRD2 Human)
   

   Tags

   EmGFP and V5

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   May 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSFFV_sfCherry3C(1-10)

   Plasmid

   #117482

   Purpose

   Lentiviral vector for constitutive expression of sfCherry3C(1-10)

   Depositor

   Bo Huang

   Article

   Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

   Insert

   sfCherry3C(1-10)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   5 substitutions (K45R, G52D, T106A, K182R, N194D)…

   Promoter

   SFFV

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1(+) - Hyperactive AID - T7 RNA Polymerase -UGI - T2A - tdTamato

    Plasmid

    #138610

    Purpose

    Express TRACE editor together with a tdTomato reporter in pcDNA3.1(+) backbone

    Depositor

    Fei Chen

    Article

    Chen et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Inserts

    Hyperactive AID
    T7 RNA Polymerase
    tdTomato
    

    Expression

    Mammalian

    Available Since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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