We narrowed to 5,560 results for: Chia

1. pPSP6+t

   Plasmid

   #48149

   Purpose

   Shuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage SP6

   Depositor

   Dieter Jahn

   Article

   Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGBW-m4136461

   Plasmid

   #149538

   Purpose

   Bacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   6xHIS;Linker_GGSG;MBP;Linker_GGSG;Cleavable TEV

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. p2CT-His-MBP-Lbu_C2c2_R1048A_H1053A

   Plasmid

   #83484

   Purpose

   Bacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 2 inactive mutant).

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

   Insert

   Lbu_C2c2_R1048A_H1053A
   

   Use

   CRISPR

   Tags

   His6-MBP

   Expression

   Bacterial

   Mutation

   R1048A, H1053A

   Promoter

   Available since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. GADD45 P-2/3m-luc

   Plasmid

   #8359

   Depositor

   Michael Greenberg

   Article

   Tran et al Science 2002 Apr 19;296(5567):530-4.

   Insert

   GADD45 promoter P-2/3m (GADD45A Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   P-2/3m

   Promoter

   Available since

   Nov. 14, 2005

   Availability

   Academic Institutions and Nonprofits only

5. pCDF-NtXNtS1R1AtR2B2

   Plasmid

   #160880

   Purpose

   Expression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1 and A. thaliana rubisco chaperone proteins raf2, and bsd2 in E. coli.

   Depositor

   Maureen Hanson

   Article

   Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

   Insert

   rbcS-S1, rbcX, raf1, raf2, bsd2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   rbcS-S1: T7, chaperones: T7

   Available since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBEST_OR2-OR1-Pr-UTR1_ClpP-T500

   Plasmid

   #153302

   Purpose

   Expresses ClpP protease in the cell-free TX-TL system

   Depositor

   Mikhail Shapiro

   Article

   Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

   Insert

   clpP (clpP Escherichia coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGBW-m4046758

   Plasmid

   #145670

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;HA

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pRJPaph-lacZYA

   Plasmid

   #67037

   Purpose

   Plasmid for Paph-lacZYA integration downstream of B. diazoefficiens (B. japonicum) scoI

   Depositor

   Julia Vorholt

   Article

   Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

   Inserts

   B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
   lacZYA
   

   Use

   Conjugative bacterial vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDF-NtXR1AtR2B2NtS1

   Plasmid

   #160879

   Purpose

   Expression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1 and A. thaliana Rubisco chaperone proteins raf2, and bsd2 in E. coli.

   Depositor

   Maureen Hanson

   Article

   Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

   Insert

   rbcS-S1, rbcX, raf1, raf2, bsd2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   rbcS-S1: pBAD, chaperones: T7

   Available since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDF-NtXNtTcR1AtR2NtB2

    Plasmid

    #160889

    Purpose

    Expression of N. tabacum Rubisco small subunit (rbcS-Tc), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.

    Depositor

    Maureen Hanson

    Article

    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

    Insert

    PT7-Nt-rbcS-Tc
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rbcS-Tc: T7, chaperones: T12

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-lxlplap

    Plasmid

    #45581

    Depositor

    Nirao Shah

    Article

    Yang et al Cell. 2013 May 9;153(4):896-909. doi: 10

    Inserts

    Green fluorescent protein
    Placental alkaline phosphatase (ALPP Human)
    

    Use

    Lentiviral

    Tags

    Histone 2B

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. T7 promoter + rpsL reporter plasmid

    Plasmid

    #156455

    Purpose

    A mutational reporter plasmid that can gain Streptomycin and/or Tetracycline resistance upon mutation. Contains T7 promoter.

    Depositor

    Matthew D. Shoulders

    Article

    Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

    Inserts

    rpsL
    TcR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    ATG start codon mutated to ACG

    Promoter

    tac and tet

    Available since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOP459

    Plasmid

    #85933

    Purpose

    Encodes the Anti-Ebola 2G4 monoclonal antibody. Chimeric - mouse variable regions, human constant regions.

    Depositor

    Timothy Lu

    Article

    Purcell et al ACS Synth Biol. 2017 Aug 17. doi: 10.102

    Insert

    Anti-Ebola 2G4 monoclonal antibody
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCph8

    Plasmid

    #22869

    Depositor

    Christopher Voigt

    Article

    Levskaya et al Nature. 2005 Nov 24. 438(7067):441-2.

    Insert

    pCph8
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    First 517 amino acids of Cph1 and the last 229 am…

    Promoter

    Available since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRJPaph-gusA

    Plasmid

    #67036

    Purpose

    Plasmid for Paph-gusA integration downstream of B. diazoefficiens (B. japonicum) scoI

    Depositor

    Julia Vorholt

    Article

    Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

    Inserts

    B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
    gusA
    

    Use

    Conjugative bacterial vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCDF-NtXNtT2R1AtR2NtB2

    Plasmid

    #160886

    Purpose

    Expression of N. tabacum Rubisco small subunit (rbcS-T2), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.

    Depositor

    Maureen Hanson

    Article

    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

    Insert

    PT7-Nt-rbcS-T2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rbcS-T2: T7, chaperones: T9

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. p2CT-His-MBP-Lbu_C2c2_R472A_H477A

    Plasmid

    #83483

    Purpose

    Bacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 1 inactive mutant).

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

    Insert

    Lbu_C2c2_R472A_H477A
    

    Use

    CRISPR

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    R472A, H477A

    Promoter

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNAIIIB rhoA WT

    Plasmid

    #61631

    Purpose

    mammalian expression of rhoA

    Depositor

    JS Gutkind

    Article

    Coso et al Cell. 1995 Jun 30;81(7):1137-46.

    Insert

    rhoA (RHOA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pOP462

    Plasmid

    #85935

    Purpose

    Encodes the Anti-Ebola 13C6 monoclonal antibody. Chimeric - mouse variable regions, human constant regions.

    Depositor

    Timothy Lu

    Article

    Purcell et al ACS Synth Biol. 2017 Aug 17. doi: 10.102

    Insert

    Anti-Ebola 13C6 monoclonal antibody
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRSET-TEV-12His

    Plasmid

    #137032

    Purpose

    empty vector; C-terminal TEV-protease-cleavable His12; T7 promoter; BseRI cloning sites (e.g., In-Fusion compatible)

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Type

    Empty backbone

    Use

    Tags

    His12 (TEV protease cleavable)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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