We narrowed to 17,584 results for: ERG

1. STR-KDEL_PGRN-SBP-mCherry

   Plasmid

   #180439

   Purpose

   Co-expresses (via IRES) streptavidin-KDEL and Progranulin-SBP-mCherry

   Depositor

   Shawn Ferguson

   Article

   Devireddy et al J Cell Biol. 2022 Feb 7;221(2):e20210404

   Insert

   Progranulin (GRN Human)
   

   Use

   Tags

   SBP-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSL1145 (pSPIN, pBBR1 backbone, R*MmeI)

   Plasmid

   #160736

   Purpose

   Single-plasmid V. cholerae CAST, encodes all proteins, crRNA, and donor DNA. Non-targeting crRNA with BsaI sites for spacer cloning. Mini-tn has MmeI site in R end for Tn-seq. pBBR1 backbone.

   Depositor

   Samuel H. Sternberg

   Article

   Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

   Insert

   VchCAST proteins, crRNA, and donor DNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pWZL Blast Snail ER

   Plasmid

   #18798

   Depositor

   Bob Weinberg

   Article

   Mani et al Cell. 2008 May 16. 133(4):704-15.

   Insert

   snail (SNAI1 Human)
   

   Use

   Retroviral

   Tags

   ER hormone binding domain

   Expression

   Mammalian

   Mutation

   G525R mutation in the ER. The presence of the G52…

   Promoter

   Available since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPB-EF1A-Puro-mCherry-RAB7

   Plasmid

   #221555

   Purpose

   Expresse Cherry-tagged human Rab7 in mammalian cells. Compatible with piggybac transposase for genome integration.

   Depositor

   Shawn Ferguson

   Article

   Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

   Insert

   Rab7A (RAB7A Human)
   

   Use

   Piggybac

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOPINB-hRNF216

   Plasmid

   #167354

   Purpose

   Expression of human RNF216 (Triad3A) codon optimized for E. coli and insect cells

   Depositor

   Bernhard Lechtenberg

   Article

   Cotton et al Mol Cell. 2022 Feb 3;82(3):598-615.e8. d

   Insert

   E3 ubiquitin-protein ligase RNF216 (RNF216 Human)
   

   Use

   Tags

   3C protease cleavage site and 6x-His tag

   Expression

   Bacterial

   Mutation

   codon optimised for expression in E. coli

   Promoter

   T7

   Available since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSL5069 (pDonor(AAVS1),CRISPR_Pse)

   Plasmid

   #200904

   Purpose

   Encodes a mini-transposon derived from Pseudoalteromonas Tn7016 with a splice acceptor-T2A-PuroR-T2A-eGFP cargo, and a CRISPR RNA under a U6 promoter. Total transposon size = 2,024 bp.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   Mini Tn7016 (AAVS1 NGS), PseCRISPR (tSL425)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR_P2R-P3_R2-Turbo-NES-mVenus-STOP-L3

   Plasmid

   #127353

   Purpose

   gateway entry vector for making C-terminal TurboID-mVenus fusion with non-nuclear proteins

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   TurboID (BirA mutant)
   

   Use

   Gateway entry vector

   Tags

   GS linker, NES, V5, and mVenus

   Expression

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Promoter

   no promoter

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. FLAG-FOXO3a TM

   Plasmid

   #8361

   Depositor

   Michael Greenberg

   Article

   Brunet et al Science 2004 Mar 26;303(5666):2011-5. Ep

   Insert

   FOXO3a (FOXO3 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   T32A, S253A, S315A

   Promoter

   Available since

   Feb. 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBW2426_pCAG-PV1-iCre-N270-L1-GID1-NLS-BGHpA

   Plasmid

   #108729

   Purpose

   Expresses N-terminus to aa270 of Cre recombinase fused to GID1 CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   Cre recombinase fragment N-terminus to aa270
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   GID1,NLS

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJR014

    Plasmid

    #198813

    Purpose

    Natural light-gated anion channels. In vivo inhibition of neurons using blue light.

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    15xUAS::GtACR2::SL2::GFP::let-858 3'UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. R4pGWB601_UBQ10p-miniTurbo-NES-YFP

    Plasmid

    #127369

    Purpose

    Binary vector for expressing cytosolic miniTurbo-YFP under the UBQ10 promoter in plants

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Tags

    GS linker, NES, V5, and YFP

    Expression

    Plant

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    Arabidopsis UBQ10 promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJ204-loxP-EF1a-mCherry-NeoR-lox2272-BGHpA-siteA-HDR

    Plasmid

    #154826

    Purpose

    Landing pad for Cre/lox-based recombinase-mediated cassette exchange (RMCE) in CHO cells, donor plasmid for CRISPR/Cas9-mediated targeted integration in CHO cells (site A)

    Depositor

    Lise Marie Grav

    Article

    Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

    Inserts

    mCherry
    NeoR
    ZsGreen1-DR
    

    Use

    CRISPR and Cre/Lox

    Tags

    PEST

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, EF1a, and SV40 early promoter

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pWZL Blast Twist ER

    Plasmid

    #18799

    Depositor

    Bob Weinberg

    Article

    Mani et al Cell. 2008 May 16. 133(4):704-15.

    Insert

    twist (Twist1 Mouse)
    

    Use

    Retroviral

    Tags

    ER hormone binding domain

    Expression

    Mammalian

    Mutation

    G525R mutation in the ER. The presence of the G52…

    Promoter

    Available since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBW2436_pCAG-PV1-GAI-L1-Cre-271C-NLS-BGHpA

    Plasmid

    #108730

    Purpose

    Expresses aa271 to C-terminus of Cre recombinase fused to GAI CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    Cre recombinase fragment aa271 to C-terminus
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    GAI and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDONR_P2R-P3_R2-miniTurbo-NES-mVenus-STOP-L3

    Plasmid

    #127355

    Purpose

    gateway entry vector for making C-terminal miniTurbo-mVenus fusion with non-nuclear proteins

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, NES, V5, and mVenus

    Expression

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    no promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBW2156_pCAG-PV1-FlpO-N27-L1-GID1-NLS-BGHpA

    Plasmid

    #108747

    Purpose

    Expresses N-terminus to aa27 of Flp recombinase fused to GID1 CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    Flp recombinase fragment N-terminus to aa27
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    GID1,NLS

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDONR_P2R-P3_R2-miniTurbo-mVenus-STOP-L3

    Plasmid

    #127356

    Purpose

    gateway entry vector for making C-terminal miniTurbo-mVenus fusion with nuclear and non-nuclear proteins

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, V5, and mVenus

    Expression

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    no promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEX-2T-GST-eIF4E K119A

    Plasmid

    #112818

    Purpose

    Expresses N-terminally GST-tagged mouse Eif4e K119A in bacterial cells

    Depositor

    Daniel Schoenberg

    Article

    Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

    Insert

    Eif4e (Eif4e Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    K119 mutated to A, increasing affinity for cap st…

    Promoter

    T7

    Available since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUG-natNT2

    Plasmid

    #110922

    Purpose

    expression of nat gene conferring resistance to nourseothricin for gene deletion in yeast

    Depositor

    Jean-Marc Daran

    Article

    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

    Insert

    nat
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    AgTEF1

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBW2176_pCAG-PV1-GAI-L1-FlpO-28C-NLS-BGHpA

    Plasmid

    #108748

    Purpose

    Expresses aa28 to C-terminus of Flp recombinase fused to GAI CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    Flp recombinase fragment aa28 to C-terminus
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    GAI and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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