166,041 results

1. TRPC1-FLAG

   Plasmid

   #24408

   Depositor

   Craig Montell

   Article

   Xu et al Cell. 1997 Jun 27. 89(7):1155-64.

   Insert

   TRPC1 (TRPC1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Aug. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. pComb8

   Plasmid

   #63889

   Purpose

   For phage display of antibody libraries by fusion to modified geneVIII.

   Depositor

   Carlos Barbas

   Article

   Barbas CF et al Proc Natl Acad Sci U S A. 1991 Sep 15;88

   Type

   Empty backbone

   Use

   Phage display

   Tags

   geneVIII

   Expression

   Bacterial

   Promoter

   lacZ

   Available Since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-EF1a-mitoGFP-DIO

   Plasmid

   #174112

   Purpose

   Cre-dependent expression of mitochondria-targeted Green Fluorescent Protein under control of the EF1a promoter

   Depositor

   Maarten Kole

   Article

   Kole et al Nat Commun. 2022 Dec 9;13(1):7598. doi:

   Has Service

   AAV2

   Insert

   mitoGFP (COX8A Human)
   

   Use

   AAV

   Tags

   Cox8 targeting sequence and GFP

   Promoter

   Ef1a

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. HT PTP-RN

   Plasmid

   #24056

   Depositor

   Frederick Stanley

   Insert

   PTPRN (PTPRN Human)
   

   Tags

   HaloTag

   Expression

   Mammalian

   Available Since

   Dec. 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pC13N-dCas9-BFP-KRAB

   Plasmid

   #127968

   Purpose

   constitutive expression of dCas9-BFP-KRAB from the CLYBL locus (Ward lab)

   Depositor

   Martin Kampmann

   Article

   Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

   Insert

   CLYBL-CAG-dCas9-NLS-BFP-KRAB
   

   Use

   TALEN

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pREDusk-MCS

   Plasmid

   #188970

   Purpose

   For red light-repressed bacterial expression of gene of interest

   Depositor

   Heikki Takala

   Article

   Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSBbi-RB

   Plasmid

   #60522

   Purpose

   SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and blasticidin resistance gene

   Depositor

   Eric Kowarz

   Article

   Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

   Type

   Empty backbone

   Use

   Transposon

   Expression

   Mammalian

   Promoter

   EF1a/RPBSA

   Available Since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pICH-chl-roGFP2-synORP1

   Plasmid

   #191701

   Purpose

   Ti plasmid pICH86988 harbouring chl-roGFP2-synORP1 for H2O2 detection in the plastids of plant cells.

   Depositor

   Philip Mullineaux

   Article

   Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

   Insert

   chl-roGFP2-synORP1
   

   Use

   Binary agrobacterium ti plasmid

   Expression

   Plant

   Promoter

   CaMV 35S promoter

   Available Since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIRESpuroN3xFLAG-UFSP2

   Plasmid

   #204553

   Purpose

   Expresses 3xFLAG-tagged UFSP2 in mammalian cells

   Depositor

   Masaaki Komatsu

   Article

   Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

   Insert

   UFSP2 (UFSP2 Human)
   

   Expression

   Mammalian

   Mutation

   none

   Available Since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-dCas9-p300 Core (D1399Y)

    Plasmid

    #61358

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation D1399Y) driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9; D1399Y mutation i…

    Promoter

    CMV

    Available Since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYTK068

    Plasmid

    #65175

    Purpose

    Encodes ConR2 as a Type 5 part to be used in the Dueber YTK system

    Depositor

    John Dueber

    Article

    Lee et al ACS Synth Biol. 2015 Apr 14.

    Insert

    ConR2
    

    Expression

    Bacterial

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCS2-MT GLI2 delta N

    Plasmid

    #17649

    Depositor

    Erich Roessler

    Article

    Roessler et al Hum Mol Genet. 2005 Aug 1. 14(15):2181-8

    Insert

    GLI2 (GLI2 Human)
    

    Tags

    6x Myc

    Expression

    Mammalian

    Mutation

    Missing 328 N-terminal amino acids

    Available Since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. pet28a-His6-Keap1

    Plasmid

    #62454

    Purpose

    His6 tagged Keap1 for E.coli expression

    Depositor

    Yimon Aye

    Article

    Parvez et al J Am Chem Soc. 2014 Dec 31.

    Insert

    Keap1 (KEAP1 Human)
    

    Tags

    His-Tag

    Expression

    Bacterial

    Available Since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRDA_174

    Plasmid

    #136476

    Purpose

    Lentiviral expression of EnAsCas12a

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

    Insert

    EnAsCas12a
    

    Use

    CRISPR and Lentiviral

    Promoter

    EF1a

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-Antares2 c-myc

    Plasmid

    #100027

    Purpose

    A BRET based CyOFP-teLuc-CyOFP fusion to further red-shift bioluminescence

    Depositor

    Huiwang Ai

    Article

    Yeh et al Nat Methods. 2017 Sep 4. doi: 10.1038/nm

    Insert

    Antares2
    

    Tags

    c-myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCre

    Plasmid

    #236212

    Purpose

    Arabinose-inducible Cre recombinase

    Depositor

    Matthew Waldor

    Article

    Basta et al Nat Microbiol. 2025 Mar 27. doi: 10.1038

    Insert

    Cre recombinase
    

    Expression

    Bacterial

    Promoter

    araBAD promoter

    Available Since

    April 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. Lenti-luciferase-P2A-Neo

    Plasmid

    #105621

    Purpose

    3rd generation lentiviral plasmid for expression of luciferase with Neo resistance

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    Luciferase
    

    Use

    Lentiviral and Luciferase

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMK444 (EF1-OsTIR1(F74G) Puro)

    Plasmid

    #214374

    Purpose

    EF1-OsTIR1(F74G) (PiggyBac)

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    EF1-OsTIR1(F74G)
    

    Expression

    Mammalian

    Promoter

    EF1 promoter

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. SpyCatcher003-mi3

    Plasmid

    #159995

    Purpose

    Expresses SpyCatcher003-mi3 in bacterial cells as virus-like particle platform for plug-and-display vaccination

    Depositor

    Mark Howarth

    Article

    Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

    Insert

    SpyCatcher003-mi3
    

    Tags

    C-Tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSEVA63_Helper

    Plasmid

    #190432

    Purpose

    M13 helper genes cloned into the pSEVA631 vector. This plasmid does not carry an M13 packaging signal. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ297.)

    Depositor

    Manish Kushwaha

    Article

    Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

    Insert

    M13 genes: I-XI
    

    Use

    Synthetic Biology

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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