159,117 results

1. pcDNA GFP10-ZIP

   Plasmid

   #182241

   Purpose

   Tripartite split-GFP mammalian. Express leucine zipper with GFP10

   Depositor

   Stéphanie Cabantous

   Article

   Cabantous et al Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038

   Insert

   GFP10-ZIP
   

   Use

   Tags

   split-GFP10 tripartite

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA ZIP-GFP11

   Plasmid

   #182242

   Purpose

   Tripartite split-GFP mammalian. Express leucine zipper with GFP11

   Depositor

   Stéphanie Cabantous

   Article

   Cabantous et al Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038

   Insert

   ZIP-GFP11
   

   Use

   Tags

   split-GFP11 tripartite

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMSCV-IRES-GFP II (pMIG II)

   Plasmid

   #52107

   Purpose

   a bicistronic IRES-GFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP

   Depositor

   Dario Vignali

   Article

   Holst et al Nat Protoc. 2006;1(1):406-17.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   IRES-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTP396

   Plasmid

   #149336

   Purpose

   Bacterial expression of His14-Biotin_acceptor_peptide-SUMOEu1-anti-GFP_nanobody

   Depositor

   Rebecca Voorhees

   Article

   Pleiner et al Science. 2020 May 21. pii: science.abb50

   Insert

   anti-GFP nanobody
   

   Use

   Tags

   Biotin acceptor peptide (Avi-tag), His14, and SUM…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. AnkyrinB-2XHA

   Plasmid

   #31057

   Depositor

   Vann Bennett

   Article

   Ayalon et al J Biol Chem. 2011 Mar 4. 286(9):7370-8.

   Insert

   Ankyrin B (ANK2 Human)
   

   Use

   Tags

   2XHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 5, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGWB551

   Plasmid

   #74881

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with G3GFP (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available since

   July 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lamp1-mScarlet-I

   Plasmid

   #98827

   Purpose

   In vivo visualization of lysosomes (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv 160374

   Insert

   Lamp1
   

   Use

   Tags

   mScarlet-I

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. p5E-mpeg1.1

   Plasmid

   #75023

   Purpose

   Can be used to drive expression in zebrafish microglia.

   Depositor

   Nicholas Cole

   Article

   Don et al Zebrafish. 2016 Sep 15.

   Insert

   macrophage expressed 1, tandem duplicate 1 (mpeg1.1 Zebrafish)
   

   Use

   5' entry vector for multisite gateway three …

   Tags

   Expression

   Mutation

   Promoter

   mpeg1.1

   Available since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGWB514

   Plasmid

   #74856

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with 3xHA (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available since

   July 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGWB401

    Plasmid

    #74795

    Purpose

    Gateway cloning compatible binary vector for simple cloning of gene.

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    No Promoter

    Available since

    July 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. Murine CD3 WTdelta-F2A-gamma-T2A-epsilon-P2A-zeta pMIA II

    Plasmid

    #52093

    Purpose

    Retroviral expression of all four WT CD3 subunits, co-expressed with mAmetrine

    Depositor

    Dario Vignali

    Article

    Holst et al Nat Immunol. 2008 Jun;9(6):658-66. doi:

    Inserts

    CD3 delta (Cd3d Mouse)
    CDC gamma (Cd3g Mouse)
    CD3 epsilon (Cd3e Mouse)
    CD3 zeta (Cd247 Mouse)
    

    Use

    Retroviral

    Tags

    F2A, IRES-EGFP, P2A, and T2A

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV.hSyn.FLP-FRT.iGluSnFR3.v857.SGZ

    Plasmid

    #196224

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857 in SGZ backbone. iGluSnFR3.v857.SGZ

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    pAAV hSyn FLP-FRT iGluSnFR3 v857.SGZ
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    IgK-chain, Myc epi tag, and Stargazin and PDZ dom…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pgRNA-CKB

    Plasmid

    #73501

    Purpose

    gRNA expression vector (with mKate2)

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Insert

    mKate2-T2A-Bsd
    

    Use

    CRISPR and Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-ccdB-V5

    Plasmid

    #214985

    Purpose

    Gateway compatible empty vector (transient mammalian expression) with a C-terminal V5 tag

    Depositor

    Wade Harper

    Type

    Empty backbone

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTRIP-SFFV-mtagBFP-2A RIGI WT

    Plasmid

    #167289

    Purpose

    Lentiviral expression construct encoding a TagBFP in frame with a self-cleaving wild-type RLR sensor RIG-I

    Depositor

    Agnel Sfeir

    Article

    Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

    Insert

    DDX58 (RIGI Human)
    

    Use

    Lentiviral

    Tags

    tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJH-MBP-CbAgo

    Plasmid

    #127707

    Purpose

    pET His6 MBP TEV CbAgo

    Depositor

    John van der Oost

    Article

    Hegge et al Nucleic Acids Res. 2019 May 9. pii: 5487

    Insert

    CbAgo
    

    Use

    Tags

    MPB

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSIN4-EF2-ABCG2-IRES-Neo

    Plasmid

    #25983

    Depositor

    Ren-he Xu

    Article

    Zeng et al Stem Cells. 2009 Oct . 27(10):2435-45.

    Insert

    ATP-binding cassette, sub-family G (WHITE), member 2, variant of isoform 2 (ABCG2 Human)
    

    Use

    Lentiviral

    Tags

    EF1-apha promoter and IRES-Neo

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Aug. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. PL-SIN-EOS-S(4+)-EGFP

    Plasmid

    #21317

    Purpose

    Lentiviral EOS reporter with Sox2 enhancer (x4), expresses EGFP

    Depositor

    James Ellis

    Article

    Hotta et al Nat Methods. 2009 May . 6(5):370-6.

    Insert

    Enhanced Green Fluorescence Protein
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EOS-S(4+) promoter region

    Available since

    June 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLentipuro3/TO/V5-GW/EGFP-Firefly Luciferase

    Plasmid

    #119816

    Purpose

    3rd generation lentiviral plasmid for expression of EGFP Firefly Luciferase fusion protein in mammalian cells

    Depositor

    Ethan Abel

    Article

    Lorenzatti Hiles et al Nat Protoc. 2019 Mar;14(3):738-755. doi:

    Inserts

    Luciferase
    Puromycin
    

    Use

    Lentiviral and Luciferase

    Tags

    EGFP and V5

    Expression

    Mammalian

    Mutation

    Stop codon removed in the frame of the V5 frame v…

    Promoter

    CMV/TO and Human Phosphoglycerate kinase

    Available since

    Jan. 2, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pcDNA3.3-hALDOA

    Plasmid

    #210809

    Purpose

    For transient transfection

    Depositor

    Sheng-cai Lin

    Article

    Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

    Insert

    ALDOA (ALDOA Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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