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pGEX-epsilon-14-3-3

Plasmid

#250063

Purpose

Bacterial expression of epsilon 14-3-3

Depositor

Mark Bedford

Article

Espejo et al J Biol Chem. 2017 Feb 10;292(6):2255-226

Insert

Epsilon (YWHAE Human)

Expression

Bacterial

Mutation

None

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pGIPZ EF1a myc tagged mbIL2 IRES miRFP720-P2A-Blast

Plasmid

#248031

Purpose

Constitutively expresses myc tagged membrane bound IL2, miRFP720 and a blasticidin resistance gene in human cells

Depositor

Joshua Leonard

Article

Deol et al GEN Biotechnol. 2023 Jun 1;2(3):228-246.

Insert

myc-tagged membrane bound IL2 (IL2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

human EF1a

Available Since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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pCEFL_GPR161-HA

Plasmid

#249268

Purpose

Expression of GPR161 wild type

Depositor

Ramiro Iglesias-Bartolome

Article

Krantz et al Cancer Res. 2025 Dec 29. doi: 10.1158/00

Insert

GPR161 (GPR161 Human)

Tags

HA

Expression

Mammalian

Promoter

EF-1

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pdCas12f1Cas_ABE-LVA

Plasmid

#220995

Purpose

Prokaryotic and constitutive expression of AsCas12f1/TadA8e-LVA fusion

Depositor

John van der Oost

Article

Swartjes et al iScience. 2025 Nov 12;28(12):114033. doi

Insert

Acidibacillus sulfuroxidans Cas12f1-ABE

Use

CRISPR

Tags

LVA

Expression

Bacterial

Mutation

D225A

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pVT-Ruby

Plasmid

#234918

Purpose

This all-in-one vector is used to express the Ruby gene

Depositor

Guoli Song

Article

Hao et al Plant Commun. 2025 May 19:101379. doi: 1

Insert

Ruby

Expression

Plant

Available Since

Dec. 8, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP VPS39

Plasmid

#245031

Purpose

Protein localization of human wild-type VPS39 in cells

Depositor

Thomas Martin

Insert

VPS39 (VPS39 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV1_Rep52-VP1

Plasmid

#232174

Purpose

Expression of replication/capsid proteins for AAV serotype 1

Depositor

Michael Wehr

Insert

Rep52, VP1 of AAV-1

Use

AAV

Expression

Mammalian

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pCSCG-TM(DPP4)-Fc-hST3Gal4 WT

Plasmid

#239825

Purpose

Displays Fc-fusion hST3Gal4 WT on mammalian cell surface via transmembrane domain of DPP4

Depositor

Sriram Neelamegham

Article

Hombu et al Small. 2025 May 26:e2502318. doi: 10.100

Insert

TM(DPP4)-Fc-hST3Gal4 WT

Use

Lentiviral

Tags

human IgG1 Fc region

Expression

Mammalian

Mutation

amino acids 1-42 of hST3Gal4 were deleted

Promoter

CMV promoter

Available Since

Aug. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_7-8_003_Amp/ColEI_mScarlet-I

Plasmid

#235969

Purpose

Ampicillin/ColEI backbone with mScarlet-I placeholder for lvl1 assembly

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

Amp/ColEI

Use

Synthetic Biology

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pUC57-FlpTag-GFP-ph0

Plasmid

#231897

Purpose

Vector with FlpTag-GFP, an inverted GFP enabling conditional tagging and visualization of endogenous proteins via Flp recombinase activity. Codon phase 0.

Depositor

Alexander Borst

Article

Fendl et al Elife. 2020 Oct 20;9:e62953. doi: 10.755

Insert

SD-GFP-SA

Expression

Bacterial

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pUC57-FlpTag-GFP-ph1

Plasmid

#231898

Purpose

Vector with FlpTag-GFP, an inverted GFP enabling conditional tagging and visualization of endogenous proteins via Flp recombinase activity. Codon phase 1.

Depositor

Alexander Borst

Article

Fendl et al Elife. 2020 Oct 20;9:e62953. doi: 10.755

Insert

SD-GFP-SA

Expression

Bacterial

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pUC57-FlpTag-GFP-ph2

Plasmid

#231899

Purpose

Vector with FlpTag-GFP, an inverted GFP enabling conditional tagging and visualization of endogenous proteins via Flp recombinase activity. Codon phase 2.

Depositor

Alexander Borst

Article

Fendl et al Elife. 2020 Oct 20;9:e62953. doi: 10.755

Insert

SD-GFP-SA

Expression

Bacterial

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pHD-FlpTag-DsRed-HDR-ph2

Plasmid

#231902

Purpose

dsDNA donor vector with 3xP3-DsRed and FlpTag-GFP for precise genomic targeting and integration of the FlpTag-GFP cassette with codon phase 2.

Depositor

Alexander Borst

Article

Fendl et al Elife. 2020 Oct 20;9:e62953. doi: 10.755

Insert

Frt-Frt14-SD-GFP-SA-Frt-Frt14

Expression

Bacterial

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pHD-FlpTag-DsRed-HDR-ph1

Plasmid

#231901

Purpose

dsDNA donor vector with 3xP3-DsRed and FlpTag-GFP for precise genomic targeting and integration of the FlpTag-GFP cassette with codon phase 1.

Depositor

Alexander Borst

Article

Fendl et al Elife. 2020 Oct 20;9:e62953. doi: 10.755

Insert

Frt-Frt14-SD-GFP-SA-Frt-Frt14

Expression

Bacterial

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pHD-FlpTag-DsRed-HDR-ph0

Plasmid

#231900

Purpose

dsDNA donor vector with 3xP3-DsRed and FlpTag-GFP for precise genomic targeting and integration of the FlpTag-GFP cassette with codon phase 0.

Depositor

Alexander Borst

Article

Fendl et al Elife. 2020 Oct 20;9:e62953. doi: 10.755

Insert

Frt-Frt14-SD-GFP-SA-Frt-Frt14

Expression

Bacterial

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pTetON-rHUH

Plasmid

#235192

Purpose

To express rHUH protein in mammalian cells

Depositor

Alice Ting

Article

Huang et al Proc Natl Acad Sci U S A. 2025 Mar 4;122

Insert

rHUH

Expression

Mammalian

Available Since

March 31, 2025

Availability

Academic Institutions and Nonprofits only

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pAG Retro EGFP Neo

Plasmid

#234464

Purpose

Control retroviral vector expressing Green Fluorescent Protein. Neomycin selection.

Depositor

Melina Fan, Addgene Research Program

Insert

Green Fluorescent Protein

Use

Retroviral

Available Since

March 21, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pBAD-WzxE-TEV-His10

Plasmid

#226781

Purpose

Expression of C-terminally TEV cleavable His10-tagged WzxE from E. coli in E. coli

Depositor

James Naismith

Article

Le Bas et al Open Biol. 2025 Jan;15(1):240310. doi: 1

Insert

wzxE

Tags

TEV cleavable 10xHistidine tag

Expression

Bacterial

Mutation

valine insertion in position 2 due to the cloning…

Promoter

araBAD

Available Since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-MfnG

Plasmid

#191115

Purpose

Lentiviral vector expressing MfnG

Depositor

Han Xiao

Article

Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

Insert

MfnG

Use

Lentiviral

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pRS313-SCT1-S288C

Plasmid

#226266

Purpose

Plasmid expressing the SCT1 allele from S288C, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SCT1 (SCT1 Budding Yeast)

Expression

Yeast

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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