8,736 results

1. EBFP2-H2B-6

   Plasmid

   #55243

   Purpose

   Localization: Nucleus/Histones, Excitation: 383, Emission: 448

   Depositor

   Michael Davidson

   Insert

   H2B (H2BC11 Human)
   

   Use

   Tags

   EBFP2

   Expression

   Mammalian

   Mutation

   D26G and V119I in H2B

   Promoter

   CMV

   Available since

   Oct. 22, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pMH-Halo tag

   Plasmid

   #154144

   Purpose

   Gateway compatible mammalian expression plasmid for halo tag fusion proteins

   Depositor

   Michael Huen

   Type

   Empty backbone

   Use

   Tags

   Halo tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET21a-ValRS-His

   Plasmid

   #124122

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Valyl-tRNA ligase (valS Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAVS1-PDi-CRISPRn

   Plasmid

   #73500

   Purpose

   Dox-inducible CRISPR nuclease (CRISPRn) knock in construct into the AAVS1 locus

   Depositor

   Bruce Conklin

   Article

   Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

   Inserts

   Cas9
   rtTA
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and TRE

   Available since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-15F11-HA-Halo

   Plasmid

   #129592

   Purpose

   The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the HaloTag. It can be used to track mature and nascent HA tagged proteins in living organism.

   Depositor

   Tim Stasevich

   Article

   Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

   Insert

   Anti-HA frankenbody-HaloTag (15F11-HA scFv-HaloTag)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. p3XFlag-CAPN1

   Plasmid

   #60941

   Purpose

   Expresses calpain1 in mammalian cells

   Depositor

   Yi Zhang

   Article

   Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

   Insert

   Calpain1 (CAPN1 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. PX551

   Plasmid

   #60957

   Purpose

   pAAV-pMecp2-SpCas9-spA (AAV-SpCas9). AAV plasmid expressing Cas9 in neurons under control of truncated mecp2 promoter.

   Depositor

   Feng Zhang

   Article

   Swiech et al Nat Biotechnol. 2014 Oct 19. doi: 10.103

   Insert

   SpCas9
   

   Use

   AAV and CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   pMecp2

   Available since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pZE27GFP

   Plasmid

   #75452

   Purpose

   Plasmid for constitutive expression of gfp

   Depositor

   James Collins

   Article

   Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

   Insert

   gfp
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   placIq promoter

   Available since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCRCT

   Plasmid

   #60621

   Purpose

   Plasmid encoding iCas9, tracrRNA and crRNAs

   Depositor

   Huimin Zhao

   Article

   Bao et al ACS Synth Biol. 2014 Sep 19.

   Inserts

   iCas9
   tracrRNA
   

   Use

   CRISPR

   Tags

   FLAG and SV40 NLS

   Expression

   Yeast

   Mutation

   changed Aspartate 147 to Tyrosine, Proline 411 to…

   Promoter

   RPR1p and TEF1p

   Available since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR-UCOE-SFFV-Zim3-dCas9-P2A-mCherry

    Plasmid

    #188779

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    Zim3 KRAB-NLS fusion

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pU6-(BbsI)_CBh-Cas9-T2A-mCherry

    Plasmid

    #64324

    Purpose

    Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to mCherry via a T2A peptide

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Inserts

    Cas9
    sgRNA cassette
    

    Use

    CRISPR

    Tags

    3xFLAG, NLS, and T2A-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. TAp63alpha-FLAG

    Plasmid

    #27008

    Depositor

    David Sidransky

    Insert

    TAp63alpha (TP63 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA3.1 hSCN5A

    Plasmid

    #145374

    Purpose

    expresses human Nav1.5 (WT) in mammalian cells

    Depositor

    Robert Kass, Hugues Abriel

    Article

    Rougier et al Am J Physiol Cell Physiol. 2005 Mar;288(

    Insert

    sodium voltage-gated channel alpha subunit 5 (SCN5A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. hIR-GFP

    Plasmid

    #22286

    Depositor

    Joseph Bass

    Article

    Ramos et al Proc Natl Acad Sci U S A. 2007 Jun 19. 1

    Insert

    INSR insulin receptor (INSR Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. FusionRed-pBAD

    Plasmid

    #54677

    Purpose

    Localization: Protein Expression Vector , Excitation: 580, Emission: 608

    Depositor

    Michael Davidson

    Article

    Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

    Type

    Empty backbone

    Use

    Tags

    6xHis and FusionRed

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

16. pAV-U6+27-Tornado-F30-Pepper(TAR Variant-2)

    Plasmid

    #129405

    Purpose

    circular Pepper RNA

    Depositor

    Samie Jaffrey

    Article

    Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

    Insert

    circular F30-Pepper(TAR Variant-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCW-LIC

    Plasmid

    #26098

    Purpose

    SGC Empty backbone for bacterial expression

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Use

    Tags

    Three consecutive Taq promoters, 2 active

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 20, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pPAmCherry-a-tubulin

    Plasmid

    #31930

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

    Insert

    PAmCherry1
    

    Use

    Tags

    a-tubulin

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 12, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFOS WT-GL3

    Plasmid

    #11983

    Purpose

    Reporter gene with mouse c-fos promoter. Induced by serum and many growth factors in quiescent cells.

    Depositor

    Ron Prywes

    Article

    Cen et al Mol Cell Biol. 2003 Sep . 23(18):6597-60

    Insert

    c-fos promoter (Fos Mouse)
    

    Use

    Tags

    Luc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLXSN18E6E7

    Plasmid

    #53459

    Purpose

    The plasmid, pLXSN18E6E7, is used to produce amphotropic recombinant retrovirus providing efficient gene transfers and expression of the HPV18 E6 and E7 genes.

    Depositor

    Denise Galloway

    Article

    Halbert et al J Virol. 1991 Jan;65(1):473-8.

    Insert

    HPV18E6E7
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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