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pT7-EGFP-C1-tubgenomic-PTC130_D

Plasmid

#146148

Purpose

Mammalian Expression of tubgenomic-PTC130

Depositor

Elisa Izaurralde

Article

Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

Insert

tubgenomic-PTC130

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsPatL1-del399-516_J

Plasmid

#146668

Purpose

Mammalian Expression of HsPatL1-del399-516

Depositor

Elisa Izaurralde

Article

Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

Insert

HsPatL1-del399-516 (PATL1 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuetP-HsPatL1_517-770-Mut1_J

Plasmid

#146672

Purpose

Bacterial Expression of HsPatL1_517-770-Mut1

Depositor

Elisa Izaurralde

Article

Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

Insert

HsPatL1_517-770-Mut1 (PATL1 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuetP-HsPatL1_517-770-Mut2_J

Plasmid

#146673

Purpose

Bacterial Expression of HsPatL1_517-770-Mut2

Depositor

Elisa Izaurralde

Article

Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

Insert

HsPatL1_517-770-Mut2 (PATL1 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuetP-HsPatL1_517-770-Mut3_J

Plasmid

#146674

Purpose

Bacterial Expression of HsPatL1_517-770-Mut3

Depositor

Elisa Izaurralde

Article

Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

Insert

HsPatL1_517-770-Mut3 (PATL1 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuetP-HsPatL1_517-770-R591AR595AK625AK626A_J

Plasmid

#146675

Purpose

Bacterial Expression of HsPatL1_517-770-R591AR595AK625AK626A

Depositor

Elisa Izaurralde

Article

Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

Insert

HsPatL1_517-770-R591AR595AK625AK626A (PATL1 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsPatL1_1-398_J

Plasmid

#146678

Purpose

Mammalian Expression of HsPatL1_1-398

Depositor

Elisa Izaurralde

Article

Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

Insert

HsPatL1_1-398 (PATL1 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsPatL1_1-517_J

Plasmid

#146679

Purpose

Mammalian Expression of HsPatL1_1-517

Depositor

Elisa Izaurralde

Article

Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

Insert

HsPatL1_1-517 (PATL1 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsPatL1_70-516_J

Plasmid

#146680

Purpose

Mammalian Expression of HsPatL1_70-516

Depositor

Elisa Izaurralde

Article

Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

Insert

HsPatL1_70-516 (PATL1 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsPatL1_353-770_J

Plasmid

#146683

Purpose

Mammalian Expression of HsPatL1_353-770

Depositor

Elisa Izaurralde

Article

Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

Insert

HsPatL1_353-770 (PATL1 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsPatL1_399-770_J

Plasmid

#146684

Purpose

Mammalian Expression of HsPatL1_399-770

Depositor

Elisa Izaurralde

Article

Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

Insert

HsPatL1_399-770 (PATL1 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-EGFP-C1-HsPatL1_518-770_J

Plasmid

#146685

Purpose

Mammalian Expression of HsPatL1_518-770

Depositor

Elisa Izaurralde

Article

Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

Insert

HsPatL1_518-770 (PATL1 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-EGFP-C1-HsPatL1-mut3_J

Plasmid

#146687

Purpose

Mammalian Expression of HsPatL1-mut3

Depositor

Elisa Izaurralde

Article

Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

Insert

HsPatL1-mut3 (PATL1 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsPatL1-mut1_I

Plasmid

#146548

Purpose

Mammalian Expression of HsPatL1-mut1

Depositor

Elisa Izaurralde

Article

Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

Insert

HsPatL1-mut1 (PATL1 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsPatL1-mut2_I

Plasmid

#146549

Purpose

Mammalian Expression of HsPatL1-mut2

Depositor

Elisa Izaurralde

Article

Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

Insert

HsPatL1-mut2 (PATL1 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsPatL1_70-770del399-516_J

Plasmid

#146663

Purpose

Mammalian Expression of HsPatL1_70-770del399-516

Depositor

Elisa Izaurralde

Article

Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

Insert

HsPatL1_70-770del399-516 (PATL1 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-dCer3-C2-dCer3

Plasmid

#191328

Purpose

Expresses the fluorescently labeled membrane anchor C2 without the chemical dimerization domain FRB as a control for recruiting proteins to plasma membrane

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

dCer3-C2-dCer3

Expression

Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-ShadowY

Plasmid

#193555

Purpose

Expresses mEGFP-ShadowY in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

ShadowY

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pE5772

Plasmid

#170779

Purpose

expression of hybrid proteins in E. coli

Depositor

Anthony Schryvers

Article

Chaudhuri et al Methods Mol Biol. 2022;2414:115-140. doi

Insert

GentR

Tags

polyhisbioMbpTEV

Expression

Bacterial

Promoter

T7

Available Since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-SpBeta-catenin

Plasmid

#185579

Purpose

to see protein dynamics

Depositor

Mamiko Yajima

Article

Wavreil et al Dev Biol. 2021 Oct;478:13-24. doi: 10.10

Insert

Beta-catenin

Use

In vitro transcription (mrna synthesis)

Tags

GFP

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-tubgenomic-PTC33_D

Plasmid

#146144

Purpose

Mammalian Expression of tubgenomic-PTC33

Depositor

Elisa Izaurralde

Article

Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

Insert

tubgenomic-PTC33

Expression

Mammalian

Available Since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-EGFP-C1-HsPatL1_70-770_J

Plasmid

#146681

Purpose

Mammalian Expression of HsPatL1_70-770

Depositor

Elisa Izaurralde

Article

Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

Insert

HsPatL1_70-770 (PATL1 Human)

Expression

Mammalian

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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C122-E15: mOrange

Plasmid

#162895

Purpose

Standard Gateway entry clone encoding mOrange fluorescent protein

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Orange fluorescent protein

Use

Synthetic Biology

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
C122-E14: mKate

Plasmid

#162894

Purpose

Standard Gateway entry clone encoding mKate fluorescent protein

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Kate fluorescent protein

Use

Synthetic Biology

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
C122-E10: mCherry

Plasmid

#162892

Purpose

Standard Gateway entry clone encoding mCherry fluorescent protein

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Cherry fluorescent protein

Use

Synthetic Biology

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
C121-E06: gLuc

Plasmid

#162890

Purpose

Standard Gateway entry clone encoding Gaussia luciferase

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Gaussia princeps luciferase

Use

Synthetic Biology

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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C121-E01: rLuc

Plasmid

#162888

Purpose

Standard Gateway entry clone encoding Renilla luciferase

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Renilla luciferase

Use

Synthetic Biology

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
C122-E54: mRuby3

Plasmid

#162899

Purpose

Standard Gateway entry clone encoding mRuby3 fluorescent protein

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Ruby3 fluorescent protein

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pDest-313 (attR4-attR3)

Plasmid

#161898

Purpose

Multisite Gateway (attR4-attR3) vector for mammalian protein expression

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Expression

Mammalian

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRB30-GFP

Plasmid

#165481

Purpose

pTriEx based vector for protein expression in mammalian cells for targets fused with C-terminal uv-GFP, Flag and His

Depositor

Renato Bruni

Article

Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

Type

Empty backbone

Tags

TEV-GFP-FLAG-10x His

Expression

Mammalian

Available Since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTricHis-PLY-WT-no charge

Plasmid

#160539

Purpose

Expression of inserted PLY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

PLY-WT-no charge

Tags

His-Tag

Expression

Bacterial

Mutation

K279A, Q280A, D283A, N284A

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTricHis-PLY-WT-charge+

Plasmid

#160538

Purpose

Expression of inserted PLY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

PLY-WT-charge+

Tags

His-Tag

Expression

Bacterial

Mutation

W278K, L282K

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTricHis-ILY-WT-N342W

Plasmid

#160518

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-WT-N342W

Tags

His-Tag

Expression

Bacterial

Mutation

N342W

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTricHis-ILY-WT-L340W

Plasmid

#160517

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-WT-L340W

Tags

His-Tag

Expression

Bacterial

Mutation

L340W

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTricHis-ILY-WT-N338W

Plasmid

#160516

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-WT-N338W

Tags

His-Tag

Expression

Bacterial

Mutation

N338W

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIS-rPAM3

Plasmid

#145803

Purpose

expresses rat PAM3 in mammalian cells

Depositor

Betty Eipper, Richard Mains

Article

Milgram et al J Cell Biol. 1994 Jan;124(1-2):33-41. do

Insert

rat PAM3

Expression

Mammalian

Mutation

See depositor comments below

Promoter

CMV

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Prham-M.PstI-p15A-aadA

Plasmid

#149340

Purpose

expresses M.PstI DNA methyltransferase in E. coli

Depositor

Ichiro Matsumura

Article

Matsumura et al PeerJ. 2020 Sep 11;8:e9841. doi: 10.7717

Insert

M.,PstI

Expression

Bacterial

Promoter

rhamnose

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
GGE139

Plasmid

#120754

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Inducible promoter for gene expression in position 2 (P2_EYK300-4AB)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

P2_EYK300-4AB

Use

Cloning/assembly plasmid

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-HiGlpG- S116A, H196A

Plasmid

#158539

Purpose

Bacterial expression of rhomboid protease catalytic mutant HiGlpG-S116A,H196A

Depositor

Sinisa Urban

Article

Mihaljevic et al Structure. 2020 Aug 10. pii: S0969-2126(

Insert

HiGlpG

Tags

GST tag

Expression

Bacterial

Mutation

S116A;H169A

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-delN-EcGlpG

Plasmid

#158537

Purpose

Bacterial expression of rhomboid protease EcGlpG without N terminus

Depositor

Sinisa Urban

Article

Mihaljevic et al Structure. 2020 Aug 10. pii: S0969-2126(

Insert

delta N-terminus EcGlpG

Tags

GST tag

Expression

Bacterial

Mutation

N-terminal deletion (1-86)

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

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