We narrowed to 8,206 results for: Dos
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Plasmid#172745PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-14; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-14
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Bs-4
Plasmid#163142PurposepLyGo cloning vector for a sequence of interest (LPMO) in B. subtilis. Vector encoding the BatLPMO10 signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterPamyL-PamyQ-PcryIIIA-cryIIIAstab49Available SinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Bs-3
Plasmid#163141PurposepLyGo cloning vector for a sequence of interest (LPMO) in B. subtilis. Vector encoding the AprE signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterPamyL-PamyQ-PcryIIIA-cryIIIAstab49Available SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Bs-2
Plasmid#163140PurposepLyGo cloning vector for a sequence of interest (LPMO) in Bacillus subtilis. Vector encoding the AmyQ signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterPamyL-PamyQ-PcryIIIA-cryIIIAstab49Available SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGG438
Plasmid#165486PurposeVector for expression of the SpCas9 VRKG variant in human cells: CMV-T7-humanVRKG(SpCas9, D1135V/S1136R/D1332K/R1333G)-1xNLS(SV40)-3xFLAGDepositorInsertMammalian codon-optimized Streptococcus pyogenes Cas9 VRKG(D1135V/S1136R/D1332K/R1333G)-1xNLS(SV40)-3xFlag
UseCRISPRTags3x FLAG and SV40 NLSExpressionMammalianMutationD1135V, S1136R, D1332K, and R1333G mutations in S…PromoterCMV and T7Available SinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGG442
Plasmid#165616PurposeVector for expression of SpCas9-Zif268 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t (KanR cassette in place of the PID)DepositorInsertTEF1p-SpCas9::KanR(in place of PID)-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t
UseCRISPRTags3x HA, SV40 NLS, Zif268, and c-Myc-like NLSExpressionYeastMutationCorrected homology relative to wild type SpCas9 (…PromoterTEF1Available SinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtS1HR1AtR2NtB2
Plasmid#160890PurposeExpression of N. tabacum Rubisco small subunit (rbcS-SH1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertPT7-Nt-rbcS-S1H
Tags6xHisExpressionBacterialPromoterrbcS-SH1: T7, chaperones: T13Available SinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtT4aR1AtR2NtB2
Plasmid#160887PurposeExpression of N. tabacum Rubisco small subunit (rbcS-T4a), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertPT7-Nt-rbcS-T4a
ExpressionBacterialPromoterrbcS-T4a: T7, chaperones: T10Available SinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEL052
Plasmid#137906PurposeOsBiP1 promoter driven Cas9-tRNA systemDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEL053
Plasmid#137907PurposeOsBiP1 promoter driven Cas9-csy4 systemDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCB6-Occludin
Plasmid#136637PurposeExpression of Occludin in mammalian cellsDepositorInsertOccludin (OCLN Chicken)
ExpressionMammalianAvailable SinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCB6-HAOccludin
Plasmid#136638PurposeExpression of HAOccludin in mammalian cellsDepositorAvailable SinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCB6-OccludinCT3
Plasmid#136639PurposeExpression of OccludinCT3 in mammalian cellsDepositorInsertOccludinCT3 (OCLN Chicken)
ExpressionMammalianMutationDeletion of C-terminal cytoplasid domainAvailable SinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCB6-HAOccludinCT3
Plasmid#136640PurposeExpression of HAOccludinCT3 in mammalian cellsDepositorInsertHAOccludinCT3 (OCLN Chicken)
TagsHA (MQDLPGNDNSTAG)ExpressionMammalianMutationDeletion of C-terminal cytoplasid domainAvailable SinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEL050
Plasmid#137904PurposeDual-mini35S driven Cas9-Csy4 systemDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEL051
Plasmid#137905PurposeDual-mini35S-enhancer driven Cas9-Csy4 systemDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
hKCNB1 R312H
Plasmid#124488Purposemissense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vectorDepositorInserthuman KCNB1 (KCNB1 Human)
TagsHAExpressionMammalianMutationchanged arginine 312 to histidineAvailable SinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0.018
Plasmid#119622PurposeLevel 0 Part. CDSDepositorInsertdCas9+deg-tag
UseSynthetic BiologyMutationbp 331 C to A , 1237 A to C, 1284 C to T, 2121 C …Available SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0.079
Plasmid#119667PurposeLevel 0 part. Terminator for the psbA2 gene Synechocystis sp. PCC 6803.DepositorInsertTpsbA2
UseSynthetic BiologyAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0.066
Plasmid#119666PurposeLevel 0 part. Terminator from pheA gene from E. coli.DepositorInsertTpheA-1
UseSynthetic BiologyAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0.080
Plasmid#119665PurposeLevel 0 part. Terminator for the cpcG1 gene Synechocystis sp. PCC 6803.DepositorInsertTcpcG1
UseSynthetic BiologyAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0.063
Plasmid#119664PurposeLevel 0 part. Terminator derived from Biobricks plasmid pSB1AK3.DepositorInsertpSB1AK3 terminator
UseSynthetic BiologyAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0.071
Plasmid#119662PurposeLevel 0 part. Terminator from tatABCD operon from E. coli.DepositorInsertECK120030798
UseSynthetic BiologyAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0.069
Plasmid#119661PurposeLevel 0 part. Terminator from arcA gene from E. coli.DepositorInsertECK120026481
UseSynthetic BiologyAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0.065
Plasmid#119659PurposeLevel 0 part. Terminator araBAD operon from E. coli.DepositorInsertECK120010860
UseSynthetic BiologyAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0.068
Plasmid#119658PurposeLevel 0 part. Terminator for clpPX gene from E. coli.DepositorInsertECK120010850
UseSynthetic BiologyAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0.072
Plasmid#119657PurposeLevel 0 part. Terminator from hyaABCDEF operon from E. coli.DepositorInsertECK120010842
UseSynthetic BiologyAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0.077
Plasmid#119656PurposeLevel 0 part. Terminator from rpoH gene from E. coli.DepositorInsertECK120010841-R
UseSynthetic BiologyAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0.064
Plasmid#119655PurposeLevel 0 part. Terminator from hupB gene from E. coli.DepositorInsertECK120010820
UseSynthetic BiologyAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
hKCNB1 G379R
Plasmid#124489Purposemissense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vectorDepositorInserthuman KCNB1 (KCNB1 Human)
TagsHAExpressionMammalianMutationchanged glycine at 379 to arginineAvailable SinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEPOR2KN0078
Plasmid#117645PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for eSaCas9(pEPOR1CB0113), sgRNA{AT1G73460}(pEPOR1CB0099) and sgRNA{AT1G73460}(pEPOR1CB0110) to Protein kinase superfamily protein.DepositorInsert[35S:eSaCas9(pEPOR1CB0113) ] +[AtU6-26:sgRNA]
ExpressionPlantAvailable SinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEPOR2KN0077
Plasmid#117644PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for SaCas9(pEPOR1CB0015) and sgRNA_Protein kinase superfamily protein {AT1G73460}(pEPOR1CB0099)and sgRNA_Protein kinase superfamily protein {AT1G73460}(pEPOR1CB0110)DepositorInsert[35S:SaCas9(pEPOR1CB0015) ] +[AtU6-26:sgRNA]
ExpressionPlantAvailable SinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
YORWΔ22Ω2
Plasmid#101705PurposeGoldenBraid vector pDGB2Ω2, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YORWΔ22 solo LTR lociDepositorTypeEmpty backboneUseYeast expressionAvailable SinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
YORWΔ22Ω1
Plasmid#101706PurposeGoldenBraid vector pDGB2Ω1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YORWΔ22 solo LTR lociDepositorTypeEmpty backboneUseYeast expressionAvailable SinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
YORWΔ22α2
Plasmid#101707PurposeGoldenBraid vector pDGB2α2, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YORWΔ22 solo LTR lociDepositorTypeEmpty backboneUseYeast expressionAvailable SinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
YORWΔ22α1
Plasmid#101708PurposeGoldenBraid vector pDGB2α1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YORWΔ22 solo LTR lociDepositorTypeEmpty backboneUseYeast expressionAvailable SinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
YPRCΔ15Ω2
Plasmid#101709PurposeGoldenBraid vector pDGB2Ω2, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRCΔ15 solo LTR lociDepositorTypeEmpty backboneUseYeast expressionAvailable SinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
YPRCΔ15α2
Plasmid#101711PurposeGoldenBraid vector pDGB2α2, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRCΔ15 solo LTR lociDepositorTypeEmpty backboneUseYeast expressionAvailable SinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
plko-tet-on puromycin-shMTH1
Plasmid#110918PurposeTo knock down MTH1 (Mut T Homolog 1) after addition of doxyclineDepositorInsertMTH1 (NUDT1 Human)
UseLentiviralAvailable SinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
KG#493
Plasmid#110932PurposeExpresses proteins in C. elegans cholinergic neurons in head, ventral nerve cord, and tailDepositorInsertsunc-17 promoter
unc-54 3' control region
ExpressionBacterialAvailable SinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
KG#94
Plasmid#110931PurposeExpresses proteins in C. elegans cholinergic neurons in head, ventral nerve cord, and tailDepositorInsertsunc-17 promoter
unc-54 3' control region
ExpressionBacterialAvailable SinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
csd2_121-308,S166A/pET28b(+)
Plasmid#83301Purposecsd2, construct: 121-308, pET28b(+) NC-tag, mutation: S166ADepositorInserthp1544
TagsHis tagExpressionBacterialMutationdeleted amino acid 1-120, and changed Serine 166 …Available SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
csd2_121-308,T151A/pET28b(+)
Plasmid#83300Purposecsd2, construct: 121-308, pET28b(+) NC-tag, mutation: T151ADepositorInserthp1544
TagsHis tagExpressionBacterialMutationdeleted amino acid 1-120, and changed Threonine 1…Available SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
csd2_121-308,Y197F/pET28b(+)
Plasmid#83299Purposecsd2, construct: 121-308, pET28b(+) NC-tag, mutation: Y197FDepositorInserthp1544
TagsHis tagExpressionBacterialMutationdeleted amino acid 1-120, and changed Tyrosine 19…Available SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
csd2_121-308,E165A/pET28b(+)
Plasmid#83298Purposecsd2, construct: 121-308, pET28b(+) NC-tag, mutation: E165ADepositorInserthp1544
TagsHis tagExpressionBacterialMutationdeleted amino acid 1-120, and changed Glutamic ac…Available SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pClneoEGFPC-let60 K16N
Plasmid#66861PurposeExpress GFP-tagged dominat negative let60, in which Lysine 16 is mutated to asparagine, in mammalian cellsDepositorInsertLet-60 (let-60 Nematode)
TagsGFPExpressionMammalianMutationLysine 16 is mutated to asparaginePromoterCMVAvailable SinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
Lifeact-Halo
Plasmid#193328Purposemammalian transient expression - expression of Lifeact-HaloDepositorInsertLifeact
ExpressionMammalianAvailable SinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
Aurein1.2_(+36GFP)-Cre
Plasmid#71748PurposeAurein1.2+36GFP-Cre fusion expression in E. ColiDepositorInsert(GGS)9_Aurein1.2+36GFP-Cre
Tags6XHisExpressionBacterialAvailable SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVRC8400-SARS-CoV-2-S2P-B.1.1.529-AVI
Plasmid#181695PurposeExpresses SARS-CoV-2 S2P protein from the B.1.1.529 (Omicron) variant with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorInsertSARS-CoV-2-S2P-B.1.1.529 (S )
TagsAVI, HRV3C, and scFcExpressionMammalianMutationA67V, ∆H69, ∆V70, T95I, G142D, ∆V143, ∆Y144, ∆Y14…Available SinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVRC8400-SARS-CoV-2-S2P-B.1.617.2-AVI
Plasmid#176334PurposeExpresses SARS-CoV-2 S2P protein from the B.1.617.2 variant with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorInsertSARS-CoV-2-S2P-B.1.617.2 (S )
TagsAVI, HRV3C, and scFcExpressionMammalianMutationT19R, G142D, del156-157, R158G, L452R, T478K, D61…Available SinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only