We narrowed to 28,253 results for: Tat

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pKH3-p190

Plasmid

#15547

Purpose

Expression of HA-tagged p190 RhoGAP

Depositor

Ian Macara

Article

Tatsis et al J Biol Chem. 1998 Dec 18. 273(51):34631-

Insert

p190 GTPase activating protein (Arhgap35 Rat)

Tags

Triple HA1 tag

Expression

Mammalian

Available Since

March 31, 2009

Availability

Academic Institutions and Nonprofits only
pMuLE (L1-L4) 4X PRE

Plasmid

#206167

Purpose

pMuLE L1-L4 entry plasmid containing 4 consensus PGR bindings sites upstream of a minimal 2YBTATA promoter

Depositor

Renee van Amerongen

Article

Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov

Insert

4X PRE

Use

Gateway cloning entry clone

Promoter

2YBTATA

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-DiLACCO1

Plasmid

#218824

Purpose

Mammalian expression of genetically encoded green fluorescent L-lactate control biosensor

Depositor

Robert Campbell

Article

Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

Insert

DiLACCO1

Expression

Mammalian

Available Since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-Flex-rev-oChIEF-tdTomato

Plasmid

#30541

Purpose

Cre-dependent viral expression of oChIEF-tdTomato for optogenetic excitation

Depositor

Rory McQuiston

Article

Bell et al Neuropharmacology. 2011 Aug 25.

Insert

oChIEF-tdtomato

Use

AAV and Cre/Lox

Tags

tdTomato

Promoter

CAG

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pPB-PNGT3-d2EGFP_CMV-mCherry

Plasmid

#213765

Purpose

Fluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.

Depositor

Gaetano Gargiulo

Article

Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

Insert

PNGT#3-d2EGFP

Use

Synthetic Biology

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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PAGFP_NS2B3

Plasmid

#195755

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Depositor

Partha Mondal

Article

Varghese et al bioRxiv 2022.12.18.520514

Insert

Dengue (Type-2) NS2B3

Tags

PA-GFP

Expression

Mammalian

Mutation

No mutations

Promoter

CMV

Available Since

Feb. 16, 2023

Availability

Academic Institutions and Nonprofits only

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Dead-Lapronic/pcDNA3.1(-)

Plasmid

#140753

Purpose

FRET-Based Genetically Encoded Nanosensor for Lactate/Pyruvate Ratio, Negative Control

Depositor

Luis Felipe Barros

Article

Galaz et al Anal Chem. 2020 Jun 29. doi: 10.1021/acs

Insert

Dead-Lapronic

Expression

Mammalian

Mutation

Changed Histidine 92 to Alanine

Promoter

CMV

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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MSCVhygro-F-Ezh2-F667I

Plasmid

#24927

Depositor

Kai Ge

Article

Wang et al Proc Natl Acad Sci U S A. 2010 Apr 20. 1

Insert

Ezh2 (EZH2 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Phenylanaline(F)667 mutated to Isoleucine(I)

Available Since

July 15, 2010

Availability

Academic Institutions and Nonprofits only
pAT222

Plasmid

#40942

Depositor

Angela Taddei

Article

Dubarry et al Genes Dev. 2011 Jul 1;25(13):1365-70.

Insert

GFP-LacI**

Tags

GFP(S65T, V163A) and SV40 NLS

Expression

Yeast

Mutation

LacI**/LacI2G(4 mutations) (see comment below); l…

Promoter

His3 promoter fragment

Available Since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-AlstR-IRES2-EGFP

Plasmid

#14895

Depositor

Edward Callaway

Article

Tan et al Neuron. 2006 Jul 20. 51(2):157-70.

Insert

AlstR (AstA-R1 Fly)

Use

AAV

Expression

Mammalian

Available Since

May 11, 2007

Availability

Academic Institutions and Nonprofits only

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pSALECTNK-TEM1(S70A,D179G)/csTEM1

Plasmid

#81163

Purpose

pSALECT plasmid with TEM-1 beta-lactamase fused to TEM-1

Depositor

Timothy Whitehead

Article

Klesmith et al Proc Natl Acad Sci U S A. 2017 Feb 14. p

Insert

TEM-1 beta-lactamase

Tags

Codon swapped TEM-1 from H26 to W290 and His6x

Expression

Bacterial

Mutation

Deleted the sec transport tag. Plasmid encodes H2…

Promoter

lac promoter

Available Since

Feb. 21, 2017

Availability

Academic Institutions and Nonprofits only

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FgH1tUTG_mup53_Ex4

Plasmid

#85534

Purpose

Inducible expression of guide RNA (mup53_Ex4) with fluorescent GFP reporter

Depositor

Marco Herold

Article

Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

Insert

mu p53

Promoter

H1t

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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TrpLE-M-nMSP1E3D1

Plasmid

#179949

Purpose

Tagless membrane scaffold protein (MSP) for formation of nanodiscs.

Depositor

Kerney Glover

Article

Julien et al Anal Biochem. 2021 Nov 27:114497. doi: 1

Insert

TrpLE-M-nMSP1E3D1

Tags

TrpLE

Expression

Bacterial

Mutation

Native Trp residues have been mutated to Phe resi…

Promoter

T7

Available Since

Feb. 23, 2022

Availability

Academic Institutions and Nonprofits only

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P447-FBP_19

Plasmid

#139710

Purpose

T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, Luz promoter is ABA responsive

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pSlH4:NnCPH:tSlH4, pSlRBCS2:AsNpga:tAtuG7, pAtuMas:NnH3H:tAtuMas, p35S:NnHisps:t35S, pAtRAB18:NnLuz:tAtuOcs

Use

Luciferase

Expression

Plant

Promoter

various

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pAG306-GPD-VMA1 chr I

Plasmid

#41896

Purpose

Yeast expression of VMA1.

Depositor

Dan Gottschling

Article

Hughes et al Nature. 2012 Dec 13;492(7428):261-5. doi

Insert

VMA1 (VMA1 Budding Yeast)

Expression

Yeast

Mutation

Contains silent mutation at 342A-G and L87S mutat…

Promoter

GPD

Available Since

April 17, 2013

Availability

Academic Institutions and Nonprofits only

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pAEMXT-deLACCO

Plasmid

#167947

Purpose

Fluorescent biosensor control for extracellular L-lactate

Depositor

Robert Campbell

Article

Nasu et al Nat Commun. 2021 Dec 6;12(1):7058. doi:

Insert

CD59-deLACCO-GPI

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

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pAM4937 (pCV0049)

Plasmid

#120082

Purpose

Cyanobacteria cloning vector. Chromosomal integration into Synechococcus elongatus neutral site 2 (NS2).

Depositor

Susan Golden

Article

Taton et al Nucleic Acids Res. 2014 Jul 29. pii: gku

Insert

Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-S7942NS2, CV-aph1, CV-ccdB-SwaI

Use

Other

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pETconNK-TEM1(S70A,D179G)

Plasmid

#81166

Purpose

pETconNK with TEM1(S70A,D179G) beta-lactamase

Depositor

Timothy Whitehead

Article

Klesmith et al Proc Natl Acad Sci U S A. 2017 Feb 14. p

Insert

TEM-1 beta-lactamase

Tags

c-myc

Expression

Bacterial and Yeast

Mutation

Deleted the sec transport tag. Plasmid encodes H2…

Promoter

GAL1 and gal1

Available Since

Feb. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pPB-MGT1-d2EGFP_CMV-mCherry

Plasmid

#213763

Purpose

Fluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.

Depositor

Gaetano Gargiulo

Article

Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

Insert

MGT#1-d2EGFP

Use

Synthetic Biology

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO {ChETA}on-WPRE

Plasmid

#111386

Purpose

AAV expression of ChETA (hChR2 with E123T/H134R mutations) fused to HA tag driven by hSynapsin promoter in Cre-ON manner for optogenetic activation

Depositor

Adam Kepecs

Article

Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

Insert

ChETA

Use

AAV

Tags

HA

Expression

Mammalian

Mutation

humanized ChR2, E123T/H134R

Promoter

hSynapsin

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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