We narrowed to 3,713 results for: stom

1. pCMV HA hRB delta CDK + T373

   Plasmid

   #58912

   Purpose

   mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T373 restored

   Depositor

   Steven Dowdy

   Article

   Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

   Insert

   Rb (RB1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   delta CDK* with T373 restored

   Promoter

   CMV

   Available Since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pAX198

   Plasmid

   #173042

   Purpose

   Custom vector for sgRNA library construction

   Depositor

   Brittany Adamson

   Article

   Hussmann et al Cell. 2021 Oct 28;184(22):5653-5669.e25.

   Insert

   HBB Gene - Second and third exons of ENST00000647020.1 (no intron) and part of the 3' UTR (HBB Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6

   Available Since

   Nov. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti-BaeI-Pgk-Cre

   Plasmid

   #173663

   Purpose

   Vector with BaeI site to allow cloning of custom sgRNA into vector containing Cre-recombinase

   Depositor

   Charles Swanton

   Article

   Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

   Insert

   BaeI site
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDG335

   Plasmid

   #100899

   Purpose

   SpCas9n (D10A Nickase mutant) with a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.

   Depositor

   Paul Thomas

   Article

   Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

   Inserts

   humanized CRISPR associated protein 9 Nickase
   U6-gRNA scaffold 1
   U6-gRNA scaffold 2
   

   Use

   CRISPR and Mouse Targeting

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D10A mutant converts to Nickase

   Promoter

   CBh and U6

   Available Since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV HA hRB delta CDK + S612

   Plasmid

   #58914

   Purpose

   mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S612 restored

   Depositor

   Steven Dowdy

   Article

   Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

   Insert

   Rb (RB1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   delta CDK* with S612 restored

   Promoter

   CMV

   Available Since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pCMV HA hRB delta CDK + S608

   Plasmid

   #58913

   Purpose

   mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S608 restored

   Depositor

   Steven Dowdy

   Article

   Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

   Insert

   Rb (RB1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   delta CDK* with S608 restored

   Promoter

   CMV

   Available Since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

7. H14_MBP_SUMO-Cys-linker-BAF

   Plasmid

   #101777

   Purpose

   Expresses His-MBP-tagged BAF in E.coli (SUMO-cleavable)

   Depositor

   Daniel Gerlich

   Article

   Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

   Insert

   BAF (BANF1) (BANF1 Human)
   

   Use

   Tags

   His14-MBP-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available Since

   Dec. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDG330

   Plasmid

   #100898

   Purpose

   SpCas9 with a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

   Depositor

   Paul Thomas

   Article

   Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

   Inserts

   humanized CRISPR associated protein 9 Nickase
   U6-gRNA scaffold 1
   U6-gRNA scaffold 2
   

   Use

   CRISPR and Mouse Targeting

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available Since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. CMV-ShadowY-CBD-P2A-Clover(T153M/F223R)-Cdc42

   Plasmid

   #165434

   Purpose

   Expresses ShadowY-CBD and Clover(T153M/F223R)-Cdc42 in mammalian cells to monitor Cdc42 activity with FLIM imaging.

   Depositor

   Hideji Murakoshi

   Article

   Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

   Insert

   ShadowY-CBD-P2A-Clover(T153M/F223R)-Cdc42
   

   Use

   Tags

   ShadowY, Clover(T153M/F223R)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR221_SLC39A8

    Plasmid

    #132177

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Polesel et al Biometals. 2022 Dec 1. doi: 10.1007/s105

    Insert

    SLC39A8 (SLC39A8 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 25, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. EHD2-T72A-mEGFP

    Plasmid

    #45934

    Purpose

    dominant-negative EHD2 mutant

    Depositor

    Ari Helenius

    Article

    Stoeber et al EMBO J. 2012 May 16;31(10):2350-64. doi:

    Insert

    Eps-15 homology domain-containing protein2 (EHD2 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    T 72 changed to A (T72A)

    Promoter

    CMV

    Available Since

    Aug. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. hSyn1-AsCpf1(RR)

    Plasmid

    #109325

    Purpose

    AAV vector expressing AsCpf1 (RR variant)

    Depositor

    Feng Zhang

    Article

    Tekin et al Nat Biomed Eng. 2018 Jul;2(7):540-554. d

    Insert

    hAsCpf1(RR)
    

    Use

    AAV

    Tags

    HA and NLS

    Expression

    Mutation

    S542R/K607R

    Promoter

    hSyn1

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJJGL004

    Plasmid

    #180608

    Purpose

    Plasmid expressing E. coli codon optimized His-MBP-TEV-PlmCasX+Region1 insertion

    Depositor

    Jennifer Doudna

    Article

    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

    Insert

    PlmCasX with DpbCasX R1 loop
    

    Use

    CRISPR

    Tags

    10xHis and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pETSUMO2_Bradyrhizobium-bGSDM

    Plasmid

    #212126

    Purpose

    For expression of the bacterial gasdermin (bGSDM) encoded by a Bradyrhizobium species. Protein is expressed with an N-terminal SUMO2 tag.

    Depositor

    Philip Kranzusch

    Article

    Johnson et al Science. 2022 Jan 14;375(6577):221-225.

    Insert

    Bradyrhizobium bGSDM
    

    Use

    Tags

    6xHis-SUMO2

    Expression

    Bacterial

    Mutation

    N-terminal methionine deleted and replaced by sin…

    Promoter

    T7

    Available Since

    Jan. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. EHD2-T72A-mCherry

    Plasmid

    #45935

    Purpose

    dominant-negative EHD2 mutant

    Depositor

    Ari Helenius

    Article

    Stoeber et al EMBO J. 2012 May 16;31(10):2350-64. doi:

    Insert

    Eps-15 homology domain-containing protein2 (EHD2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    T 72 changed to A (T72A)

    Promoter

    CMV

    Available Since

    Aug. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDONR221_SLC6A2

    Plasmid

    #131891

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Sijben et al Sci Rep. 2021 Jun 10;11(1):12290. doi: 1

    Insert

    SLC6A2 (SLC6A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 9, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pPuroR-MCS-LexA-VP16-MiniWhite

    Plasmid

    #165898

    Purpose

    Puromycin resistant LexA driver vector with Mini-white CDS eye marker. Contains an enhancer grammar GB20 entry point for custom enhancers. Standard cut-and-paste cloning can also be used. Vector uses Purple-White bacteria colony screening for identification of correct cloning

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Selectable Empty Enhancer Driver
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV HA hRB delta CDK + S780

    Plasmid

    #58915

    Purpose

    mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S780 restored

    Depositor

    Steven Dowdy

    Article

    Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

    Insert

    Rb (RB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta CDK* with S780 restored

    Promoter

    CMV

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pCAG-mEGFP-CaMKIIa (T305D/T306D)

    Plasmid

    #127611

    Purpose

    Fluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T305D/T306D)

    Depositor

    Ryohei Yasuda

    Article

    Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

    Insert

    calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    changed Threonine 305/306 to Alanine

    Promoter

    pCAG

    Available Since

    Jan. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR221_SLC39A14

    Plasmid

    #132105

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Polesel et al Biometals. 2022 Dec 1. doi: 10.1007/s105

    Insert

    SLC39A14 (SLC39A14 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 3, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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