We narrowed to 6,511 results for: itch
-
Plasmid#231567PurposeExpresses components of the LOOMINA optogenetic transcriptional control system.DepositorInsertdCas9-AsLOV2x3
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pOSV00157
Plasmid#222937PurposeKill switch plasmid for DNA sensor construction using Bacillus subtilisDepositorInsertTxpA-RatA toxin-antitoxin
UseTagsExpressionBacterialMutationPromoterPhyperspankAvailable sinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
scFv-HaloTag-iLID
Plasmid#227390PurposeAdhesion module, scFv binds SunTagDepositorInsertscFv-HaloTag-iLID
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
BRD4dN-mCherry-SspB
Plasmid#227387PurposeCondensate module, light-inducible oligomerization IDRDepositorInsertBRD4 IDR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-LOVPLD-mCherry-OSBP(PH)
Plasmid#219561PurposeGolgi-targeted LOVPLDDepositorInsertLOVPLD-mCherry-OSBP(PH)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-lyn10-mCherry-dLOVPLD
Plasmid#219556PurposePM-targeted catalytically dead LOVPLDDepositorInsertlyn10-mCherry-dLOVPLD
UseTagsmCherryExpressionMammalianMutationH170A mutation on PLDPromoterAvailable sinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-p18-mCherry-LOVPLD
Plasmid#219560Purposelysosome-targeted LOVPLDDepositorInsertp18-mCherry-LOVPLD
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBbA5k-AcrAB-AsLOV2*(543)
Plasmid#210863PurposeIPTG inducible promoter (lacUV5) expressing AcrA and AcrB-AsLOV2*(543). AsLOV2*(543) is also described in the literature as LOVdeg.DepositorInsertsacrA
acrB
UseSynthetic BiologyTagsAsLOV2*(543)ExpressionMutationPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Opto-BMPR2a
Plasmid#207616PurposeZebrafish BMPR2a BMP receptor kinase domain and CTD fused to VfLOVDepositorInsertBMPR2a kinase domain and CTD + VfLOV domain (bmpr2a Zebrafish, Vaucheria frigida)
UseTagsExpressionMutationPromoterAvailable sinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
T1T/pUBQ10(mTurq->mScarlet)
Plasmid#195947PurposeTp901 target with pUBQ10 switching from mTurq to mScarletDepositorInserttRBCS-mScarlet-attP_Tp901-pUBQ10-attB_Tp901-mTurq-tUBQ10
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
T1B/pUBQ10(mTurq->mScarlet)
Plasmid#195946PurposeBxb1 target with pUBQ10 switching from mTurq to mScarletDepositorInserttRBCS-mScarlet-attP_Bxb1-pUBQ10-attB_Bxb1-mTurq-tUBQ10
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT22U6CatalE4PCh(#767)
Plasmid#184078Purposecyclofen-inducible Catalase activation in zebrafish permanent transgenicDepositorInsertCatalDeltaC-ERT2-P2A-mCherry
UseZebrafish transgenesis (blue eyes) + in vitro tra…TagsExpressionMutationCatalDeltaC: deleted 516-527; ERT2: G400V, M543A,…PromoterZf-Ubi+SP6Available sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNUT2
Plasmid#175745PurposeSingle-component photo-inducible (LOV2-based) nucleolus-targeting tool 2DepositorInsertmCherry-LOV2-NoLS (human nuclear factor-κB inducing kinase)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT22i5uasH3En2SE4P3Che(#1212)
Plasmid#184098Purposecyclofen-inducible chicken EN2 (mut) activation in zebrafish permanent transgenicDepositorInsert3HA-chkEn2(C>S)-ERT2-5'fP2A-mCherry
UseZebrafish transgenesis (blue eyes)Tags3xHAExpressionMutationchkEn2: C175S; 5'fP2A: GSG-P2A; ERT2: G400V,…Promoter5xUASAvailable sinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT22i5uasm5En25EE4P3Che(#1213)
Plasmid#184099Purposecyclofen-inducible chicken EN2 (mut) activation in zebrafish permanent transgenicDepositorInsert5myc-chkEn2(5E)-ERT2-5'fP2A-mCherry
UseZebrafish transgenesis (blue eyes)Tags5xMycExpressionMutationchkEn2: S150E, S151E, S153E, S155E, S156E; ERT2: …Promoter5xUASAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT22i5uasm5En2SE4P3Che(#1209)
Plasmid#184097Purposecyclofen-inducible chicken EN2 (mut) activation in zebrafish permanent transgenicDepositorInsert5myc-chkEn2(C>S)-ERT2-5'fP2A-mCherry
UseZebrafish transgenesis (blue eyes)Tags5xMycExpressionMutationchkEn2: C175S; 5'fP2A: GSG-P2A; ERT2: G400V,…Promoter5xUASAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT22cUm5En2SE4P3Che(#1189)
Plasmid#184095Purposecyclofen-inducible chicken EN2 (mut) activation in zebrafish permanent transgenicDepositorInsert5myc-chkEn2(C>S)-ERT2-5'fP2A-mCherry
UseZebrafish transgenesis (blue eyes)Tags5xMycExpressionMutationchkEn2: C175S; 5'fP2A: GSG-P2A; ERT2: G400V,…PromoterZf-UbiAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT22cUm5En2E4P3Che(#1188)
Plasmid#184094Purposecyclofen-inducible chicken EN2 (wt) activation in zebrafish permanent transgenicDepositorInsert5myc-chkEn2-ERT2-5'fP2A-mCherry
UseZebrafish transgenesis (blue eyes)Tags5xMycExpressionMutation5'fP2A: GSG-P2A; ERT2: G400V, M543A, L544A, …PromoterZf-UbiAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT22i5uasm5En2E4P3Che(#1208)
Plasmid#184096Purposecyclofen-inducible chicken EN2 (wt) activation in zebrafish permanent transgenicDepositorInsert5myc-chkEn2-ERT2-5'fP2A-mCherry
UseZebrafish transgenesis (blue eyes)Tags5xMycExpressionMutation5'fP2A: GSG-P2A; ERT2: G400V, M543A, L544A, …Promoter5xUASAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
IBAR-Cry2PHR
Plasmid#176604PurposeCry2PHR with N-terminal IBAR domainDepositorInsertIBAR-Cry2PHR-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHIS8-3_DL121
Plasmid#171953PurposeExpression vector for ecDHFR-asLOV2 fusion protein. Also has TYMSDepositorInsertFusion of ecDHFR and asLOV2 at position 121 of DHFR
UseTags8-His tagExpressionBacterialMutationPromoterT7Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_DL121_WTTS
Plasmid#171954PurposeSelection vector for chimeric fusion of ecDHFR and asLOV2.DepositorInsertsFusion of ecDHFR and asLOV2 at position 121 of DHFR
Thymidylate Synthase
UseTags8-His tagExpressionBacterialMutationPromoterT7Available sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCh-LaGFP(N)-cpFRB1-T2A-FKBP-LaGFP(C)
Plasmid#165029PurposeExpress cpFRB1-based GFP specific Chessbody (chemically switchable split nanobody); visualized by mCherryDepositorInsertLaGFP(N)-cpFRB1-T2A-FKBP-LaGFP(C)
UseTagsmCherryExpressionBacterial and MammalianMutationPromoterT7Available sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-LaM(N)-cpFRB1-T2A-FKBP-LaM(C)
Plasmid#165026PurposeExpress cpFRB1-based mCherry specific Chessbody (chemically switchable split nanobody); visualized by GFPDepositorInsertLaM8(N)-cpFRB1-T2A-FKBP-LaM8(C)
UseTagsGFPExpressionBacterial and MammalianMutationPromoterT7Available sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-LaM(N)-cpFRB2-T2A-FKBP-LaM(C)
Plasmid#165028PurposeExpress cpFRB2-based mCherry specific Chessbody (chemically switchable split nanobody); visualized by GFPDepositorInsertLaM8(N)-cpFRB2-T2A-FKBP-LaM8(C)
UseTagsGFPExpressionBacterial and MammalianMutationPromoterT7Available sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCSIIbsr_2pabPAC
Plasmid#158971Purpose2P-activatable bPAC for lentivirusDepositorInsert2pabPAC
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
CRY2olig-BFP-YTHDF1(D401N)-C
Plasmid#155354PurposeLentivector to express human YTHDF1(264-559) with D401N mutation and CRY2olig-BFP at the N-terminalDepositorInsertYTHDF1(264-559)
UseLentiviralTagsExpressionMammalianMutationD401NPromoterAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQP-iRISB
Plasmid#138979PurposeLight-controlled tri-directional targeting of endogenous proteinsDepositorInsertNcoI-BphP1-CAAX-IRES2-NES-SGlinkeriB( GFP)-mCherry-Q-PAS1-AsLOV2cNLS-NotI
UseTagsExpressionMutationPromoterAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQP-2363
Plasmid#138975PurposeRelocalization of genomically expressed protein of interest from cytoplasm to nucleus upon near-infrared illuminationDepositorInsertNcoI-BphP1- iB(GFP) -IRES2-NES-mCherry-QPAS1- NLS-NotI
UseTagsExpressionMutationPromoterAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFLinkC-AI(G3)-PDZ(GPG-G)FN3(GGS4)-TVMV
Plasmid#137117PurposeProtease Switch Clone ID: 2-A5DepositorInsertAI(G3)-PDZ(GPG-G)FN3(GGS4)-TVMV
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSP64-EMTB-GFP-ePDZ
Plasmid#135600PurposeExpression of EMTB-GFP-ePDZ mRNA for microinjection for use of optogenetics in S. purpuratusDepositorInsertEMTB-GFP-ePDZ
UseIn vitro transcription (mrna synthesis)TagsEGFP and ePDZExpressionMutationPromoterUnknownAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSP64-Lifeact-GFP-ePDZ
Plasmid#135602PurposeExpression of Lifeact-GFP-ePDZ mRNA for microinjection to be used for optogenetics in S. purpuratusDepositorInsertLifeact-GFP-ePDZ
UseIn vitro transcription (mrna synthesis)TagsEGFP and ePDZExpressionMutationPromoterUnknownAvailable sinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMM0622
Plasmid#128997PurposepTEF1-Zif268DBD-CRY2PHR cassette plasmid flanked by ConL1 and ConR2DepositorInsertConL1-pTEF1-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-ConR2 AmpR-ColE1
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0529
Plasmid#128986PurposePart plasmid for Zif268DBD-CRY2, Type 3 (coding sequence) partDepositorInsertSV40NLS-ZiF268DBD-CRY2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0658
Plasmid#129019PurposepREV1-Zif268DBD-CRY2 cassette plasmid flanked by ConL1 and ConR2DepositorInsertConL1-pRNR2-SV40NLS-ZiF268DBD-CRY2-tSSA1-ConR2-ColE1-AmpR
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0557
Plasmid#128991PurposeLEU2 integration vector for Zif268DBD-CRY2DepositorInsertUra3 3' homology-pTDH3-SV40NLS-ZiF268-CRY2-tADH1-URA3-Ura3 5' homology
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0558
Plasmid#128992PurposeLEU2 integration vector for Zif268DBD-CRY2PHRDepositorInsertUra3 3' homology-pTDH3-SV40NLS-ZiF268-CRY2PHR-tADH1-URA3-Ura3 5' homology
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0657
Plasmid#129018PurposepRPL18B-Zif268DBD-CRY2 cassette plasmid flanked by ConL1 and ConR2DepositorInsertConL1-pRPL18B-SV40NLS-ZiF268DBD-CRY2-tSSA1-ConR2-ColE1-AmpR
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0656
Plasmid#129017PurposepTEF1-Zif268DBD-CRY2 cassette plasmid flanked by ConL1 and ConR2DepositorInsertConL1-pTEF1-SV40NLS-ZiF268DBD-CRY2-tSSA1-ConR2-ColE1-AmpR
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0317
Plasmid#128979PurposeEncodes Zif268DBD-CRY2 under pscADH1DepositorInsertpscADH-SV40NLS-Zif268DBD-CRY2 (L3)-tscADH1 scTRP1
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only