We narrowed to 4,947 results for: mcs

1. pDONR-53BP1 1-1972 WT

   Plasmid

   #53446

   Purpose

   Recombinational donor/master vector

   Depositor

   Daniel Durocher

   Article

   Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

   Insert

   53BP1 (TP53BP1 Human)
   

   Use

   Gateway

   Mutation

   siRNA resistant and mutations A128V, T720A, A1347…

   Available Since

   April 10, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pLenti6_YAP1-V5

   Plasmid

   #173002

   Purpose

   constitutive expression of V5-tagged YAP1

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   YAP1-V5 (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Tol2 8xSTAR-mNeonGreen. PGK-puro

   Plasmid

   #136255

   Purpose

   Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

   Depositor

   Hugo Snippert

   Article

   Oost et al Cell Rep. 2018 Feb 6;22(6):1600-1614. do

   Insert

   stem cell Ascl2 reporter, 8 repeats
   

   Use

   Tol2 transposase

   Tags

   mNeonGreen

   Expression

   Mammalian

   Available Since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. t1-435

   Plasmid

   #166578

   Purpose

   For expression of human talin head (residues 1-435) in E. coli. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.

   Depositor

   Vesa Hytönen

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2020 Dec 22;11

   Insert

   Tln1 Head (1-435) (TLN1 Human)
   

   Tags

   His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…

   Expression

   Bacterial

   Mutation

   residues 1-435 only

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCW57.1-FLAG-SRSF3_Full.Length_Codon.Optimized-Blast

   Plasmid

   #171951

   Purpose

   All-in-one lentiviral vector for doxycycline-inducible expression of FLAG-tagged, codon-optimized, full-length SRSF3

   Depositor

   Sujatha Jagannathan

   Article

   Campbell et al Cell Rep. 2023 Jun 13;42(6):112642. doi:

   Insert

   serine and arginine rich splicing factor 3 (SRSF3 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   codon-optimized

   Promoter

   TRE

   Available Since

   July 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. LentiCas9-sgVRK1 #4-Blast

   Plasmid

   #199647

   Purpose

   Expresses Cas9 and sgRNA guide targeting VRK1

   Depositor

   William Hahn

   Article

   So et al JCI Insight. 2022 Oct 10;7(19):e158755.

   Insert

   N/A (VRK1 Human)
   

   Use

   Lentiviral

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV7.1_3xFLAG-WWC1

   Plasmid

   #172994

   Purpose

   constitutive expression of FLAG-tagged WWC1

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   3xFLAG-WWC1 (WWC1 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. lentiCas9-Venus

   Plasmid

   #70267

   Purpose

   Expresses human codon-optimized S. pyogenes Cas9 protein and Venus reporter from EFS promoter. Lentiviral backbone.

   Depositor

   Daniel Bauer

   Article

   Canver et al Nature. 2015 Sep 16. doi: 10.1038/nature

   Inserts

   Cas9
   Venus Reporter
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   EFS-NS and P2A

   Available Since

   Feb. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-3XHA-Zmat3

   Plasmid

   #167343

   Purpose

   Expresses HA-tagged Zmat3

   Depositor

   Laura Attardi

   Article

   Bieging-Rolett et al Mol Cell. 2020 Nov 5;80(3):452-469.e9. d

   Insert

   Zinc finger matrin-type 3 (Zmat3 Mouse)
   

   Tags

   3HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA 3.1 AURKA Lys162Met

    Plasmid

    #157751

    Purpose

    Expression of kinase-dead AURKA K62M in mammalian cells

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Expression

    Mammalian

    Mutation

    AURKA K162M is a kinase-dead version of AURKA

    Promoter

    CMV

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1(+)-NES-ZapCV2 (cpV143)

    Plasmid

    #112060

    Purpose

    Genetically-encoded Cyan-Yellow Zinc FRET sensor. Localizes to the cytosol. Useful for detecting free zinc ion levels in the cytosol (in vitro Kd ~2.3 nM, n = 0.53)

    Depositor

    Amy Palmer

    Article

    Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi

    Insert

    NES-ZapCV2
    

    Tags

    Nuclear Export Signal (NES) derived from HIV-1

    Expression

    Mammalian

    Mutation

    ECFP gene is truncated 36 bp at 3' end. Venu…

    Promoter

    CMV

    Available Since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTRIP-SFFV-mtagBFP-2A STING 368STOP

    Plasmid

    #102590

    Purpose

    Lentivector to express STING delta 368 and mTagBFP

    Depositor

    Nicolas Manel

    Article

    Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

    Insert

    STING (STING1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    delta 368

    Promoter

    SFFV

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA-FRT/T0-eGFPnls-53BP1 1220-1631 WT

    Plasmid

    #60814

    Purpose

    mammalian expression vector

    Depositor

    Daniel Durocher

    Article

    Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

    Insert

    53BP1 (TP53BP1 Human)
    

    Tags

    eGFP-NLS (SV40)

    Expression

    Mammalian

    Available Since

    April 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pAAV-hSyn-NES-NIR-GECO1

    Plasmid

    #113683

    Purpose

    Express NIR-GECO1 preferentially in neurons

    Depositor

    Robert Campbell

    Article

    Qian et al Nat Methods. 2019 Feb;16(2):171-174. doi

    Insert

    NES-NIR-GECO1
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. FcD265A-FLAG

    Plasmid

    #65420

    Purpose

    Mammalian expression plasmid for murine Fc-FLAG, used for monomeric Fc-IL2 production

    Depositor

    Dane Wittrup

    Article

    Zhu et al Cancer Cell. 2015 Apr 13;27(4):489-501.

    Insert

    FcD265A-FLAG
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D265A

    Promoter

    Modified CMV Promoter

    Available Since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pTRIP-SFFV-mtagBFP-2A STINGHAQ

    Plasmid

    #102587

    Purpose

    Lentivector to express STING HAQ and mTagBFP

    Depositor

    Nicolas Manel

    Article

    Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

    Insert

    STING (STING1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    R71H-G230A-R293Q (HAQ)

    Promoter

    SFFV

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTRIP-SFFV-mtagBFP-2A STING-354STOP

    Plasmid

    #102589

    Purpose

    Lentivector to express STING delta 354 and mTagBFP

    Depositor

    Nicolas Manel

    Article

    Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

    Insert

    STING (STING1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    delta 354

    Promoter

    SFFV

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTwist-SARS-CoV-2 Δ18 P.1

    Plasmid

    #173476

    Purpose

    Encodes SARS-CoV-2 P.1 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

    Insert

    SARS-CoV-2 Spike Truncated to remove 18 amino acids
    

    Expression

    Mammalian

    Available Since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP-MYO10-HMM

    Plasmid

    #87256

    Purpose

    Expression plasmid to perform NanoSPD assays in mammalian cells (generates EGFP-tagged bait).

    Depositor

    Thomas Friedman

    Article

    Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

    Insert

    MYO10 (Myo10 Mouse)
    

    Expression

    Mammalian

    Mutation

    Myo10 is truncated to contain aa.1-941

    Promoter

    CMV

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCR8-PHF19

    Plasmid

    #68864

    Purpose

    GW entry vector for PHF19

    Depositor

    Adrian Bracken

    Article

    Brien et al Nat Struct Mol Biol. 2012 Dec;19(12):127

    Insert

    PHF19 (PHF19 Human)
    

    Use

    Gateway entry vector

    Promoter

    none

    Available Since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only