We narrowed to 15,732 results for: LEA

1. p2B-55

   Plasmid

   #177924

   Purpose

   Expresses optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast TDH3 promoter

   Depositor

   Vadim Gladyshev

   Article

   Barre et al (unpublished)

   Insert

   Optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast TDH3 promoter
   

   Expression

   Bacterial and Yeast

   Mutation

   V9I; F67S; G72P, F86Y; R171K

   Promoter

   TDH3

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHJ320

   Plasmid

   #20671

   Depositor

   Stephen Shaw

   Article

   Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

   Insert

   Moesin (MSN Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   March 31, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA3_Cas7-11_T2A_Csx29

   Plasmid

   #197607

   Purpose

   Expresses Cas7-11 and Csx29 in mammalian cells

   Depositor

   Feng Zhang

   Article

   Strecker et al Science. 2022 Nov 25;378(6622):874-881.

   Insert

   Cas7-11_T2A_Csx29
   

   Expression

   Mammalian

   Available Since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMSSM24: pCMV-SpuFz1

   Plasmid

   #205260

   Purpose

   Human expression vector for human codon optimized SpuFz1

   Depositor

   Feng Zhang

   Article

   Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

   Insert

   SpuFz1
   

   Expression

   Mammalian

   Available Since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. psgRNAv1-empty

   Plasmid

   #171611

   Purpose

   sgRNA_v1 plasmid for AsCas12f1 in bacteria

   Depositor

   Quanjiang Ji

   Article

   Wu et al Nat Chem Biol. 2021 Sep 2. pii: 10.1038/

   Insert

   AsCas12f1_sgRNA_1
   

   Expression

   Bacterial

   Available Since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pST50Trc1-HISNDHFR

   Plasmid

   #63940

   Purpose

   Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

   Depositor

   Song Tan

   Article

   Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

   Type

   Empty backbone

   Tags

   6xHis; TEV cleavable

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pST50Trc3-HISNDHFR

   Plasmid

   #63976

   Purpose

   Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

   Depositor

   Song Tan

   Article

   Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

   Type

   Empty backbone

   Tags

   6xHis; TEV cleavable

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pST50Trc4-HISNDHFR

   Plasmid

   #63994

   Purpose

   Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

   Depositor

   Song Tan

   Article

   Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

   Type

   Empty backbone

   Tags

   6xHis; TEV cleavable

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pST50Trc2-HISNDHFR

   Plasmid

   #63958

   Purpose

   Position 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

   Depositor

   Song Tan

   Article

   Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

   Type

   Empty backbone

   Tags

   6xHis; TEV cleavable

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. BsaI-tagRFP-BsaI-pUC-kan

    Plasmid

    #170771

    Purpose

    test plasmid for Golden Gate assembly, encodes fluorescent red protein

    Depositor

    Ichiro Matsumura

    Article

    Matsumura et al Biotechniques. 2022 May;72(5):185-193. d

    Insert

    tagRFP
    

    Promoter

    none

    Available Since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. FUGW-VGAT-pHluorin

    Plasmid

    #207467

    Purpose

    Lentiviral expression of VGAT-pHluorin

    Depositor

    Robert Edwards

    Article

    Jain et al bioRxiv 2023

    Insert

    VGAT (Slc6a12 Rat)
    

    Use

    Lentiviral

    Tags

    pHluorin

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBabe(LTR)-Cytochrome C-GFP

    Plasmid

    #41183

    Depositor

    Douglas Green

    Article

    Goldstein et al Nat Cell Biol. 2000 Mar;2(3):156-62.

    Insert

    Cytochrome C-GFP
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    Nov. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKDC.154

    Plasmid

    #222228

    Purpose

    Acceptor plasmid for human retron-Eco1 variants.

    Depositor

    Seth Shipman

    Article

    Crawford et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pST1374

    Plasmid

    #13426

    Depositor

    Keith Joung

    Article

    Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

    Type

    Empty backbone

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Feb. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKLV-U6gRNA-EF(BbsI)-PGKpuro2ABFP

    Plasmid

    #62348

    Purpose

    An empty optimized gRNA expression vector

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Nucleic Acids Res. 2015 Jun 19. pii: gkv

    Insert

    none
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p3s-Cas9HC

    Plasmid

    #43945

    Depositor

    Jin-Soo Kim

    Article

    Cho et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    HA epitope and NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. p4xEGFP-N1

    Plasmid

    #122164

    Purpose

    Expression of 4xEGFP

    Depositor

    Georg Mayr

    Article

    Bohm et al Anal Biochem. 2017 Sep 15;533:48-55. doi

    Insert

    4xEGFP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLEXY (pDN137)

    Plasmid

    #72656

    Purpose

    golden gate entry vector for mCherry-LEXY tagging of proteins (no constitutive NLS)

    Depositor

    Barbara Di Ventura, Roland Eils

    Article

    Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.

    Type

    Empty backbone

    Use

    Golden gate entry vector

    Tags

    mCherry-LEXY

    Expression

    Mammalian

    Promoter

    CMV-F

    Available Since

    Feb. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. SUMO_TwinStrep_Cas7-11_crRNA

    Plasmid

    #197612

    Purpose

    For protein purification of Cas7-11

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2022 Nov 25;378(6622):874-881.

    Insert

    SUMO_TwinStrep_Cas7-11 with crRNA
    

    Expression

    Bacterial

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEF-I-GFP GX

    Plasmid

    #45443

    Depositor

    John Brigande

    Article

    Gubbels et al Nature. 2008 Sep 25. 455(7212):537-41.

    Type

    Empty backbone

    Tags

    IRES EGFP

    Expression

    Mammalian

    Promoter

    human EF1α

    Available Since

    July 2, 2013

    Availability

    Academic Institutions and Nonprofits only