Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 7,655 results for: Pol

Clear keywords
Showing: 2741 - 2760 of 7655 results

  1. pGEM-NLS-Cre-2A-mCherry-FKF

    Plasmid
    #119868
    Purpose
    Donor cassette containing NLS-tagged Cre, a Thosea asigna virus peptide (2A), mCherry and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene
    Depositor
    Tatjana Sauka-Spengler
    Publication
    Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d
    Insert
    NLS-Cre, 2A, mCherry and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    Tags
    mCherry
    Expression
    Bacterial
    Available since
    April 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    119868-plasmid-map-sequence-id-240979

    Enlarge

  2. pOCC72

    Plasmid
    #118866
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal monomeric GFP, cleavable with TEV
    Depositor
    Marc Bickle, PEPC MPI CBG
    Publication
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C-mGFP-TEV-NotI-ccdB-AscI-stop-HindIII cassette
    Tags
    HIS6, cleavable with 3C protease, mGFP, cleavable…
    Expression
    Insect
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118866-plasmid-map-sequence-id-232323

    Enlarge

  6. p405MET3

    Plasmid
    #15962
    Depositor
    Nicolas Buchler, Fred Cross
    Insert
    MET3 promoter + polylinker + CYC1 terminator (MET3 Budding Yeast)
    Expression
    Bacterial and Yeast
    Mutation
    MET3 promoter is between SacI and XbaI restrictio…
    Available since
    Nov. 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    15962-plasmid-map-sequence-id-8447

    Enlarge

  8. pHCMV_POWV_PrME

    Plasmid
    #207299
    Purpose
    Mammalian expression of Tick-borne powassan virus PrME protein
    Depositor
    Feng Zhang
    Publication
    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    POWV_PrME (POLY Tick-borne powassan virus)
    Expression
    Mammalian
    Mutation
    mammalian codon optimization
    Promoter
    CMV promoter
    Available since
    Nov. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    207299-plasmid-map-sequence-id-407424

    Enlarge

  11. TAF1L

    Plasmid
    #39014
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    TAF1L (TAF1L Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  14. tet-pLKO.puro_shGAC

    Plasmid
    #110423
    Purpose
    shGAC, silence glutaminase GAC isoform, doxycycline inducible, puromycin selection
    Depositor
    Sandra Martha Gomes Dias
    Insert
    GLS glutaminase (GLS Human)
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Promoter
    H1/TO (RNA PolIII)
    Available since
    June 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    110423-plasmid-map-sequence-id-213314

    Enlarge

  19. pBIG2abc_nsp12-His6-3xFlag/nsp7/nsp8 (SARS-CoV-2)

    Plasmid
    #169185
    Purpose
    Baculoviral transfer vector to co-express nsp12-His6-3xFlag, nsp7 and nsp8 (SARS-CoV-2) in insect cells
    Depositor
    John Diffley
    Publication
    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443
    Insert
    nsp12-His6-3xFlag/nsp7/nsp8 (ORF1ab Synthetic, SARS-CoV-2)
    Tags
    His6-3xFlag (nsp12 C-terminus)
    Expression
    Insect
    Mutation
    Codon optimised for insect cells (Spodoptera frug…
    Promoter
    Polyhedrin
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169185-plasmid-map-sequence-id-334217

    Enlarge

Showing: 2741 - 2760 of 7655 results