Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 29,613 results for: des.2

Showing: 2741 - 2760 of 29613 results

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pCZGY1584 Pcol-19::ebp-2::mGFP::unc-54 3'UTR

Plasmid

#129774

Purpose

Marker of microtubule dynamics in C. elegans hypodermis

Depositor

Andrew Chisholm

Article

Chuang et al Elife. 2016 Sep 23;5. doi: 10.7554/eLife

Inserts

Pcol-19
ebp-2 (ebp-2 Nematode)
unc-54 3'UTR

Use

Synthetic Biology; Gateway

Tags

Synthetic Intron and mGFP

Expression

Worm

Promoter

Pcol-19

Available Since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pSEM234 - pEXP[Pmlc-1 | mCherry(2xNLS) | cbr-tbb-2 UTR]

Plasmid

#159795

Purpose

Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

Insert

pEXP[Pmlc-1 | mCherry(2xNLS) | cbr-tbb-2 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pMDJ13 - pEXP[Peft-3 | tagRFP-T (NLS) | tbb-2 UTR]

Plasmid

#159800

Purpose

Expression construct with ubiquitous expression to test fluorescence level on microscope

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Peft-3 | tagRFP-T (NLS) | tbb-2 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSEM230 - pEXP[Pmlc-1 | ceGFP(2xNLS) | cbr-tbb-2 UTR]

Plasmid

#159794

Purpose

Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

Insert

pEXP[Pmlc-1 | ceGFP(2xNLS) | cbr-tbb-2 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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R702-X31-566: His6-MBP-tev-G-Hs.PDE6D(2-150)

Plasmid

#159689

Purpose

E. coli protein expression of His6-MBP-tev-G-Hs.PDE6D(2-150)

Depositor

Dominic Esposito

Article

Dharmaiah et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

Insert

His6-MBP-tev-G-Hs.PDE6D(2-150) (PDE6D Human)

Tags

His6-MBP-tev

Expression

Bacterial

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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R702-X80-635: His6-tev-Hs.NF1opt (1-2818) isoform 2

Plasmid

#159575

Purpose

Baculovirus protein expression of His6-tev-Hs.NF1opt (1-2818) isoform 2

Depositor

Dominic Esposito

Article

Sherekar et al J Biol Chem. 2020 Jan 24;295(4):1105-111

Insert

His6-tev-Hs.NF1opt (1-2818) isoform 2 (NF1 Human)

Tags

His6-tev

Expression

Insect

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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R791-X12-636: His6-MBP-tev-Hs.NF1 ABC (2-1540)

Plasmid

#159577

Purpose

Baculovirus protein expression of His6-MBP-tev-Hs.NF1 ABC (2-1540)

Depositor

Dominic Esposito

Article

Sherekar et al J Biol Chem. 2020 Jan 24;295(4):1105-111

Insert

His6-MBP-tev-Hs.NF1 ABC (2-1540) (NF1 Human)

Tags

His6-MBP-tev

Expression

Insect

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 G75V (GTA) T76I (ATT)

Plasmid

#212443

Purpose

Encodes SARS-CoV-2 spike G75V, T76I for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike G75V, T76I (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

G75V, T76I

Promoter

CAG

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 BA.4 (V3G,S658N)

Plasmid

#212504

Purpose

Encodes SARS-CoV-2 variant BA.4 Spike V3G,S658N for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike BA.4 (contains third aa mutation and without N658S) (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 D405N (AAC) R408S (AGC)

Plasmid

#212496

Purpose

Encodes SARS-CoV-2 spike D405N, R408S for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike D405N, R408S (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

D405N, R408S

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 N679K (AAA) P681H (CAC)

Plasmid

#212480

Purpose

Encodes SARS-CoV-2 spike N679K, P681H for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike N679K, P681H (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

N679K, P681H

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 N440K (AAG) G446S (AGC)

Plasmid

#212469

Purpose

Encodes SARS-CoV-2 spike N440K, G446S for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike N440K, G446S (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

N440K, G446S

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 S477N (AAC) T478K (AAA)

Plasmid

#212470

Purpose

Encodes SARS-CoV-2 spike S477N, T478K for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike S477N, T478K (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

S477N, T478K

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 L452Q (CAA) F490S (TCT)

Plasmid

#212449

Purpose

Encodes SARS-CoV-2 spike L452Q, F490S for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike L452Q, F490S (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

L452Q, F490S

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 K417N (AAT) L452R (CGT)

Plasmid

#212435

Purpose

Encodes SARS-CoV-2 spike K417N, L452R for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike K417N, L452R (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

K417N, L452R

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 L452R (CGT) T478K (AAA)

Plasmid

#212437

Purpose

Encodes SARS-CoV-2 spike L452R, T478K for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike L452R, T478K (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

L452R, T478K

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 (P.1) Triple RBD

Plasmid

#212412

Purpose

Encodes SARS-CoV-2 spike K417T,E484K,N501Y for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike K417T,E484K,N501Y (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

K417T,E484K,N501Y

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 K417T (ACG) E484K (AAA)

Plasmid

#212382

Purpose

Encodes SARS-CoV-2 spike E484K for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike E484K (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

E484K

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 K417T (ACG) N501Y (TAT)

Plasmid

#212383

Purpose

Encodes SARS-CoV-2 spike N501Y for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike N501Y (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

N501Y

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 Q239R (CGA) D614G (GGT)

Plasmid

#212378

Purpose

Encodes SARS-CoV-2 spike Q239R, D614G for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike Q239R, D614G (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

Q239R, D614G

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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