We narrowed to 50,951 results for: son

1. pEMS1710

   Plasmid

   #29296

   Purpose

   Pleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428).

   Depositor

   Elizabeth Simpson

   Article

   de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

   Insert

   Ple238 (TRH Human)
   

   Use

   Pleiades promoter project [sic, pleaides plieades]

   Tags

   Intron-LacZ-NLS

   Available Since

   June 10, 2011

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pCMV-hERalpha

   Plasmid

   #101141

   Purpose

   Mammalian expression of full-length human estrogen receptor-alpha

   Depositor

   Elizabeth Wilson

   Article

   Mao et al J Biol Chem. 2008 May 9;283(19):12819-30

   Insert

   estrogen receptor-alpha (ESR1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-mDlx-GCaMP6f-Fishell-2

   Plasmid

   #83899

   Purpose

   GCaMP6f expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer element

   Depositor

   Gordon Fishell

   Article

   Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

   Has Service

   AAV Retrograde, AAV1, and AAV9

   Insert

   GCaMP6f
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   mDlx

   Available Since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB-GFP-Gal8

   Plasmid

   #127191

   Purpose

   Piggybac transposon plasmid with CAG promoter GFP-Galectin 8 (GFP-Gal8) fusion protein. Useful as genetically encoded endosomal escape sensor.

   Depositor

   Jordan Green

   Article

   Rui et al Sci Adv. 2019 Dec 6;5(12):eaay3255. doi:

   Insert

   GFP-Gal8 (LGALS8 Human)
   

   Tags

   Gal8 is fused to the c-terminus of GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-nSpCas9-TadA8e

   Plasmid

   #209044

   Purpose

   Lentiviral vector expressing doxycycline-inducible human codon-optimized adenine base editor where TadA8e (ABE8e) is fused to the N-terminus of SpCas9(D10A)-6xNLS

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   TadA8e-nSpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   Tight TRE promoter

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-nSpCas9-TadA8.20m

   Plasmid

   #209045

   Purpose

   Lentiviral vector expressing doxycycline-inducible human codon-optimized adenine base editor where TadA8.20m is fused to the N-terminus of SpCas9(D10A)-6xNLS

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   TadA8.20m-nSpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   Tight TRE promoter

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-mDlx-GFP-Fishell-1

   Plasmid

   #83900

   Purpose

   GFP expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer element

   Depositor

   Gordon Fishell

   Article

   Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

   Has Service

   AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   eGFP
   

   Use

   AAV

   Promoter

   mDlx

   Available Since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-mDlx-ChR2-mCherry-Fishell-3

   Plasmid

   #83898

   Purpose

   ChR2-mCherry fusion expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer element

   Depositor

   Gordon Fishell

   Article

   Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

   Has Service

   AAV Retrograde, AAV1, and AAV9

   Insert

   ChR2
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   mDlx

   Available Since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET01-TAF5-ex8-Minigene-SRSF1 motif MUT

   Plasmid

   #218973

   Purpose

   Vector for constitutive expression of TAF5 alternative exon-8 mini gene with mutated SRSF1 motif (AGTCTCCT)

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   TAF5 exon-8 (with mutant SRSF1 motif )and the native flanking intronic sequences spanning the exon-8
   

   Expression

   Mammalian

   Mutation

   mutant SRSF1 binding motif (AGTCTCCT)

   Promoter

   RSV LTR

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-CBE4max-SpRYc-P2A-EGFP

    Plasmid

    #208336

    Purpose

    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRYc

    Depositor

    Pranam Chatterjee

    Article

    Zhao et al Nat Commun. 2023 Oct 4;14(1):6175. doi:

    Insert

    human codon optimized CBE4max Cas9 variant named SpRYc with P2A-EGFP
    

    Use

    CRISPR

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    Sc++ (1-1119), SpRY (1111-1368), D10A

    Promoter

    CAG

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX6P1‐hsRNASEH2BCA

    Plasmid

    #108692

    Purpose

    For expression in E coli of N-terminally GST-tagged human RNASEH2B and untagged RNASEH2C and A, allowing affinity purification of the RNase H2 trimeric enzyme

    Depositor

    Martin Reijns, Andrew Jackson

    Article

    Reijns et al J Biol Chem. 2011 Mar 25;286(12):10530-9

    Inserts

    RNASEH2B (RNASEH2B Human)
    RNASEH2C (RNASEH2C Human)
    RNASEH2A (RNASEH2A Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Shine Dalgarno sequence upstream of start codon a…

    Promoter

    tac

    Available Since

    April 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR223_S100A13_WT_V5

    Plasmid

    #82975

    Purpose

    Gateway Donor vector containing S100A13, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Matthew Meyerson, Jesse Boehm

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    S100A13 (S100A13 Human)
    

    Use

    Gateway entry vector

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. ABL1_HUMAN_D0

    Plasmid

    #79727

    Purpose

    This plasmid encodes the kinase domain of ABL1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, John Chodera, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    ABL1 (ABL1 Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pRM053 His-TEV-Cas9 triNLS 0C

    Plasmid

    #196244

    Purpose

    Expression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)

    Depositor

    Ross Wilson

    Article

    Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    HA, His6 (N terminal on backbone), NLS, Nucleopla…

    Expression

    Bacterial

    Mutation

    Cys at positions 80 and 574 have been mutated to …

    Available Since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDECKO_GFP

    Plasmid

    #141210

    Purpose

    empty GFP vector for cloning sgRNA or pgRNAs

    Depositor

    Rory Johnson

    Article

    Esposito et al Cell Genom. 2022 Aug 22;2(9):100171. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX-6p1-GST-ArcΔCTD

    Plasmid

    #119878

    Purpose

    Bacterial Expression construct for the production and purification of Arc missing C-terminal Domain

    Depositor

    Jason Shepherd

    Article

    Pastuzyn et al Cell. 2018 Mar 22;173(1):275. doi: 10.10

    Insert

    Activity Regulated Cytoskeletal Protein (Arc Rat)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Deletion of nt 832-1110

    Promoter

    tac

    Available Since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX6P1‐hsRNASEH2BCA(D34A/D169A)

    Plasmid

    #108693

    Purpose

    For expression in E coli of N-terminally GST-tagged human RNASEH2B and untagged RNASEH2C and A (with D34A and D169A catalytic site mutations) for purification of the RNase H2 trimeric enzyme

    Depositor

    Martin Reijns, Andrew Jackson

    Article

    Reijns et al J Biol Chem. 2011 Mar 25;286(12):10530-9

    Inserts

    RNASEH2B (RNASEH2B Human)
    RNASEH2C (RNASEH2C Human)
    RNASEH2A-D34A/D169A
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Shine Dalgarno sequence upstream of start codon, …

    Promoter

    tac

    Available Since

    April 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR223_PYGL_WT_V5

    Plasmid

    #82991

    Purpose

    Gateway Donor vector containing PYGL, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Matthew Meyerson, Jesse Boehm

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    PYGL (PYGL Human)
    

    Use

    Gateway entry vector

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBS-EF1-IRES-NEO-Control

    Plasmid

    #80757

    Purpose

    Transposon Reporter

    Depositor

    Alex Kentsis

    Article

    Henssen et al Nat Genet. 2017 May 15. doi: 10.1038/ng.

    Insert

    NeoR (neoR Synthetic)
    

    Promoter

    EF1-IRES from PB-EF1-IRES-NEO

    Available Since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR223_STK11_p.D176H

    Plasmid

    #82870

    Purpose

    Gateway Donor vector containing STK11, part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Matthew Meyerson, Jesse Boehm

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    STK11 (STK11 Human)
    

    Use

    Gateway entry vector

    Mutation

    D176H

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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