58,077 results

1. pTreTight-Htt94Q-CFP

   Plasmid

   #23966

   Depositor

   Nico Dantuma

   Article

   Maynard et al Proc Natl Acad Sci U S A. 2009 Aug 18. 1

   Insert

   N-terminal fragment Huntingtin Q94 (HTT Human)
   

   Use

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   94 polyQ repeat

   Promoter

   Available since

   April 15, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRK172-6xHis-0N4R-P301S-tau-HiBiT

   Plasmid

   #187835

   Purpose

   Expression of 6xHis-tagged P301S tau of 0N4R isoform with a C-terminal HiBiT tag for bimolecular luminescence with LgBiT.

   Depositor

   William McEwan

   Article

   Tuck et al Cell Rep. 2022 May 3;39(5):110776. doi:

   Insert

   6XHis-0N4R-P301S-tau-HiBiT
   

   Use

   Tags

   6xHistidine and HiBiT

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDH-CMV-human MYCN-EF1-mRFP

   Plasmid

   #193708

   Purpose

   Lentiviral expression of human MYCN

   Depositor

   Martine Roussel

   Insert

   MYCN (MYCN Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pComb3XLambda

   Plasmid

   #63892

   Purpose

   Backbone pComb3X containing PCR template for human antibody lambda light chain constant region

   Depositor

   Carlos Barbas

   Article

   Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):

   Insert

   human antibody lambda light chain (IGLL5 Human)
   

   Use

   Phage display

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lacZ

   Available since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. mCherry-Occludin-N-10

   Plasmid

   #55112

   Purpose

   Localization: Tight Junctions, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   Occludin (OCLN Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMyrPalm_mEGFP

   Plasmid

   #21037

   Depositor

   Daniel Gerlich

   Article

   Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

   Insert

   pMyrPalm
   

   Use

   Tags

   mEPFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   May 12, 2009

   Availability

   Academic Institutions and Nonprofits only

7. MAPK1

   Plasmid

   #73248

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   MAPK1 (MAPK1 Human)
   

   Use

   Tags

   His-6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. mCherry-Ykt6

   Plasmid

   #209888

   Purpose

   Expresses mCherry tagged Ykt6 in mammalian cells

   Depositor

   Geert van den Bogaart

   Insert

   Ykt6
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. hRad51(A89E)–Cas9(D10A)

   Plasmid

   #125563

   Purpose

   Expresses the Rad51(A89E)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter

   Depositor

   David Liu

   Article

   Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

   Insert

   Rad51(A89E)–Cas9(D10A) (RAD51 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3 - hSRSF6

    Plasmid

    #120419

    Purpose

    overexpression of human SRSF6

    Depositor

    Gillian Bates, Andreas Neueder

    Article

    Neueder et al Nat Commun. 2018 Sep 27;9(1):3955. doi:

    Insert

    SRSF6 (SRSF6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV immediate/early

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. Gi2-CASE

    Plasmid

    #168121

    Purpose

    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi2. Composed of the subunits G alpha i2 (GNAI2) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).

    Depositor

    Gunnar Schulte

    Article

    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

    Insert

    GNAI2, GNB1, GNG2 (GNAI2, GNB1, GNG2 Human)
    

    Use

    Luciferase

    Tags

    cpVenus on GNG2

    Expression

    Mammalian

    Mutation

    NLuc is inserted at C112/E115 within GNAI2

    Promoter

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. PB-PARP1-Apex2–eGFP

    Plasmid

    #211555

    Purpose

    A piggyBac vector containing CMV-PARP1-Apex2-eGFP-IRES-Neo cassette.

    Depositor

    Chris Lord

    Article

    Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

    Insert

    PARP1 (PARP1 Human)
    

    Use

    Piggybac

    Tags

    Apex2-eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pmiRGLO Dual-Luciferase-MYB-3'UTR

    Plasmid

    #182250

    Purpose

    Human MYB 3` UTR region containing 3x binding sites for miR-150 cloned downstream of luciferase reporter gene

    Depositor

    Silvia Monticelli

    Article

    Chirichella et al PLoS Biol. 2022 Feb 10;20(2):e3001538. d

    Insert

    3' UTR region of MYB gene
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti6.2_mCherry_Grx1_roGFP2

    Plasmid

    #155045

    Purpose

    Fluorescent redox reporter

    Depositor

    Vanessa LaPointe

    Insert

    Glutaredoxin-1 (GLRX Human)
    

    Use

    Lentiviral

    Tags

    roGFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMIEG3-c-Jun

    Plasmid

    #40348

    Depositor

    Alexander Dent

    Article

    Wang et al J Immunol. 2005 Feb 15;174(4):2098-105.

    Insert

    c-Jun (JUN Human)
    

    Use

    Retroviral

    Tags

    IRES eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. FUGW-Cytosol-TurboID

    Plasmid

    #200961

    Purpose

    3rd gen lentiviral plasmid for expressing cytosol localized TurboID with EGFP

    Depositor

    Keriann Backus

    Article

    Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

    Insert

    Cytosol-TurboID
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. HA-BACE1-mBFP

    Plasmid

    #196697

    Purpose

    Expresses Bace1 with an HA tag at N-terminal for single molecule tracking and other measusers in mammalian cells

    Depositor

    Martino Calamai

    Article

    Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

    Insert

    Beta-secretase 1 (BACE1 Human)
    

    Use

    Tags

    HA and mBFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mNeonGreen-DEVD-NanoLuc

    Plasmid

    #98287

    Purpose

    caspase-3 BRET Sensor

    Depositor

    Maarten Merkx

    Article

    den Hamer et al ACS Sens. 2017 Jun 23;2(6):729-734. doi:

    Insert

    mNeonGreen-LEHD-NanoLuc
    

    Use

    Tags

    His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. TFORF2459

    Plasmid

    #142089

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    IRF1 (IRF1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV5B-Flag-Smad3

    Plasmid

    #11742

    Depositor

    Jeff Wrana

    Article

    Labbe et al Mol Cell. 1998 Jul . 2(1):109-20.

    Insert

    SMAD3 (SMAD3 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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