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pXPR_206

Plasmid

#96920

Purpose

for SaCas9 knockout, lentiviral expression of S. aureus Cas9 (SauCas9) and gRNA scaffold

Depositor

John Doench, David Root

Publication

Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pEFIRES-P-EGFP-ADRP

Plasmid

#87157

Purpose

Fluorescent protein localized to lipid droplets (LDs)

Depositor

Elina Ikonen

Publication

Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

Insert

ADRP (PLIN2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

mCherry-R TTGGTCACCTTCAGCTTGG

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBK-VRER-BE3

Plasmid

#85173

Purpose

Expresses VRER-BE3 in mammalian cells

Depositor

David Liu

Publication

Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Insert

VRER-BE3

Expression

Mammalian

Promoter

CMV

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pBK-YE1-BE3

Plasmid

#85174

Purpose

Expresses YE1-BE3 in mammalian cells

Depositor

David Liu

Publication

Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Insert

YE1-BE3

Expression

Mammalian

Promoter

CMV

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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mKO2-SLBP(18-126)

Plasmid

#83914

Purpose

Fluorescent probe for S/G2 cell cycle transition

Depositor

Michael Lin

Publication

Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

mKO2-SLBP(18-126) (SLBP Human, Synthetic)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pJKR-H-tetR

Plasmid

#62561

Purpose

Tetracycline biosensor. High copy plasmid that expresses GFP proportional to tetracycline concentration.

Depositor

George Church

Publication

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Expression

Bacterial

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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MIP 339 CoMiP coLin28 2A Nanog IRES eGFP

Plasmid

#63729

Purpose

To express codon optimized Lin-28 and wild type Nanog. A single plasmid reprogramming system using a mini-intronic plasmid (MIP).

Depositor

Mark Kay, Joseph Wu

Publication

Diecke et al Sci Rep. 2015 Jan 28;5:8081. doi: 10.103

Inserts

Lin28 (LIN28A Human)
Nanog (NANOG Human)

Tags

T2A

Expression

Mammalian

Mutation

codon optimized

Promoter

SFFV

Available since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

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pMSCV-mCherry-Syk

Plasmid

#50045

Purpose

Expression of mCherry-Syk in mammalian cells

Depositor

Hidde Ploegh

Publication

Strijbis et al PLoS Pathog. 2013 Jun;9(6):e1003446. doi

Insert

Spleen tyrosine kinase (Syk) (Syk Mouse)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Promoter

pCMV

Available since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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E1b-GFP-Tol2

Plasmid

#37845

Depositor

Nadav Ahituv

Publication

Birnbaum et al Genome Res. 2012 Jun;22(6):1059-68. Epub

Type

Empty backbone

Use

Zebrafish enhancer assay vector

Promoter

E1b

Available since

Oct. 1, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pLKO.1-sh-hSOX9-1

Plasmid

#40644

Depositor

Bob Weinberg

Publication

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

sh-hSOX9-1 (SOX9 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

hU6

Available since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pCMV-Tag2B EDD C2768A

Plasmid

#37189

Depositor

Darren Saunders, Charles Watts

Publication

Henderson et al J Biol Chem. 2002 Jul 19;277(29):26468-7

Insert

EDD C2768A (UBR5 Human)

Tags

6x His Tag and FLAG

Expression

Mammalian

Mutation

Changed Cysteine 2768 to Alanine

Promoter

CMV

Available since

July 26, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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CHMP2A_GFP_N_term

Plasmid

#31805

Depositor

Daniel Gerlich

Publication

Guizetti et al Science. 2011 Mar 25;331(6024):1616-20.

Insert

CHMP2A (CHMP2A Human)

Expression

Mammalian

Available since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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tdp43-EGFP construct4

Plasmid

#28197

Depositor

Zuoshang Xu

Publication

Yang et al PLoS One. 2010 . 5(12):e15878.

Insert

Tar DNA binding protein (TARDBP Human)

Tags

EGFP

Expression

Mammalian

Mutation

aa216-414 only

Available since

Sept. 8, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pDONR223-ZAP70

Plasmid

#23887

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ZAP70 (ZAP70 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pWZL Neo Myr Flag PTK2

Plasmid

#20610

Depositor

William Hahn, Jean Zhao

Publication

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PTK2 (PTK2 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pBTR1

Plasmid

#22468

Depositor

Gary Pielak

Publication

Olteanu et al Biochem Biophys Res Commun. 2003 Dec 19.

Insert

cytochrome c (CYCS Human)

Expression

Bacterial

Available since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pmRFP_LAP2beta_IRES_puro2b

Plasmid

#21047

Depositor

Daniel Gerlich

Publication

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

LAP2_beta (TMPO Rat)

Tags

mRFP

Expression

Bacterial and Mammalian

Available since

July 14, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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GFP-mROCK2

Plasmid

#101296

Purpose

Mammalian expression of Rock2-GFP

Depositor

Alpha Yap

Publication

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Insert

Rock2 (Rock2 Mouse)

Expression

Mammalian

Promoter

CMV

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pAcGFP1-N1-Dsup

Plasmid

#90020

Purpose

To express GFP-tagged Dsup in mammalian cells transiently

Depositor

Takekazu Kunieda

Publication

Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

Insert

Dsup

Tags

AcGFP1

Expression

Mammalian

Promoter

CMV

Available since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pWR63

Plasmid

#203331

Purpose

episomal Cas9 and sgRNA expression vector without sgRNA for S. stipitis

Depositor

J. Brian Robertson

Publication

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
CCW12 promoter (S. stipitis)
CaCas9
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1
THD3 promoter (S. stipitis)
tRNA-sgRNA(SapI)-HDV
ADH1 terminator (S. stipitis)

Use

CRISPR

Expression

Yeast

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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