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We narrowed to 14,168 results for: eng

Showing: 2761 - 2780 of 14168 results
  1. pG1C

    Plasmid
    #71740
    Purpose
    Modular shuttle vector for Geobacillus and E. coli
    Depositor
    Type
    Empty backbone
    Use
    Synthetic Biology; Shuttle vector
    Expression
    Bacterial
    Available since
    July 14, 2016
    Availability
    Academic Institutions and Nonprofits only
  2. pShHELIX_sgRNA_entry (CJT32)

    Plasmid
    #181782
    Purpose
    Expresses ShHELIX containing a nicking I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Insert
    nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
  3. CRBN_ΔHBD

    Plasmid
    #232779
    Purpose
    Expresses CRBN_ΔHBD sequence in E. coli cells with cleavable MBP-His tag and deleted internal DDB1 binding domain
    Depositor
    Insert
    Cereblon (CRBN Human)
    Tags
    MBP-His6-TEV
    Expression
    Bacterial
    Mutation
    CRBN (residues 47 to 193 and 249 to 436) with GNG…
    Promoter
    T7
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
  4. gGBEv6.3-SpCas9

    Plasmid
    #202629
    Purpose
    vector for encoding an engineered glycosylase-based guanine base editor (gGBEv6.3) with SpCas9 nickase driven by EF1a promoter, guide RNA compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Insert
    nSpCas9(D10A)-MPGv6.3
    Expression
    Mammalian
    Mutation
    MPGv6.3 = G163R, N169G, D175R, C178N, S198A, K202…
    Promoter
    hU6, EF1a, CBh
    Available since
    June 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
Showing: 2761 - 2780 of 14168 results