We narrowed to 4,947 results for: mcs

1. pETM30-C9ORF78_F8A

   Plasmid

   #193369

   Purpose

   Expression of C9ORF78 F8A variant in E.coli with N-terminal His6-GST-tag which is cleavable with TEV protease

   Depositor

   Markus Wahl

   Article

   Bergfort et al Nat Commun. 2022 Mar 3;13(1):1132. doi:

   Insert

   C9ORF78_F8A (C9orf78 Human)
   

   Tags

   His6-GST-TEV

   Expression

   Bacterial

   Mutation

   F8A, the mutation is located in the binding inter…

   Promoter

   T7

   Available Since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-TRE-DIO-tdTomato-f

   Plasmid

   #127092

   Purpose

   An AAV genome with tet-inducible, Cre-dependent expression of farnesylated (f) tdTomato

   Depositor

   Viviana Gradinaru

   Article

   Rajendran et al Nat Commun. 2019 Apr 26;10(1):1944. doi:

   Insert

   tdTomato-f
   

   Use

   AAV

   Tags

   farnesylation signal from c-Ha-Ras

   Expression

   Mammalian

   Available Since

   June 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. AKAP1-EGFP-uTEV1

   Plasmid

   #171005

   Purpose

   HiLITR protease with AKAP1 N-terminal tmd targeting information (Mitochondria)

   Depositor

   Alice Ting

   Article

   Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

   Insert

   AKAP1(tmd)-EGFP-uTEV1 (AKAP1 Synthetic)
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Promoter

   pTRE-tight (TetOn)

   Available Since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSRW1JZ

   Plasmid

   #129528

   Purpose

   C. elegans codon-optimized FlincG3 (GFP-based cGMP sensor) expressed in C. elegans ASE neuron pair

   Depositor

   Noelle L'Etoile

   Article

   Woldemariam et al Genetics. 2019 Jul 22. pii: genetics.119

   Insert

   FlincG3
   

   Expression

   Worm

   Available Since

   Sept. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFP-RAB10 K154A

   Plasmid

   #130886

   Purpose

   Expresses human RAB10 K154A mutant with N-terminal GFP tag

   Depositor

   Michael Bassik

   Article

   Jeng et al Cell Host Microbe. 2019 Oct 9;26(4):551-

   Insert

   RAB10 (RAB10 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   K154A

   Available Since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTRIP-SFFV-mtagBFP-2A STING V155M 342STOP

   Plasmid

   #102594

   Purpose

   Lentivector to express STING V155M delta 342 and mTagBFP

   Depositor

   Nicolas Manel

   Article

   Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

   Insert

   STING (STING1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   V155M delta 342

   Promoter

   SFFV

   Available Since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lenti-EFS-hTSC1-T2A-Bsd

   Plasmid

   #193199

   Purpose

   Lentiviral vector expressing human TSC1 cDNA (driven by EFS promoter) and a blasticidin resistance marker

   Depositor

   Julien Sage

   Article

   Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

   Insert

   TSC1 (TSC1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. MKV937/nlp-1p::FlincG3

   Plasmid

   #140508

   Purpose

   This construct contains cGMP biosensor driven by nlp-1 promoter for expression in PHB sensory neuron in pSMdelta backbone.

   Depositor

   Miri VanHoven

   Article

   Woldemariam et al Genetics. 2019 Jul 22. pii: genetics.119

   Insert

   FlincG3
   

   Expression

   Worm

   Promoter

   nlp-1

   Available Since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-NeoR_dCas9-Sniper-KRAB_sgSTK3i_#2 (NeoR)

   Plasmid

   #209775

   Purpose

   CRISPRi for STK3

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   dCas9-Sniper-KRAB, sgSTK3i (STK3 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTRIP-SFFV-mtagBFP-2A STINGHAQ V155M

    Plasmid

    #102592

    Purpose

    Lentivector to express STING V155M HAQ and mTagBFP

    Depositor

    Nicolas Manel

    Article

    Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

    Insert

    STING (STING1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    V155M R71H-G230A-R293Q

    Promoter

    SFFV

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNeuLite PEA3 mt

    Plasmid

    #16248

    Depositor

    Mien-Chie Hung

    Article

    Xing et al Nat Med. 2000 Feb . 6(2):189-95.

    Insert

    Her-2/neu promoter (ERBB2 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    PEA3 mt: PEA3 motif AGGAAG changed to AGCTCG

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

12. MDH1-miR-31SM-PGK-GFP-Blast

    Plasmid

    #31516

    Depositor

    Chang-Zheng Chen

    Article

    Arnold et al Genome Res. 2011 May . 21(5):798-810.

    Insert

    miR-31 (Mir31 Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    seed mutant control

    Available Since

    Sept. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

13. LADL Bridge + Target Zfp462-Klf4SE

    Plasmid

    #127668

    Purpose

    Encodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter; 2 gRNAs targeting the Zfp462 promoter and 2 gRNAs targeting the Klf4 SE expressed from their individual hU6 promoters

    Depositor

    Jennifer Phillips-Cremins

    Article

    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

    Insert

    CRY2-HA-2A-mCherry and gRNAs 129, 135, 115 and 117
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Promoter

    EF1a and hU6

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lenti-sgNotch2#2/Cre

    Plasmid

    #193226

    Purpose

    Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Notch2 gene

    Depositor

    Julien Sage

    Article

    Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

    Insert

    sgNotch2#2 (Notch2 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-SwitchON-sgCh2-2

    Plasmid

    #199637

    Purpose

    Tamoxifen-inducible expression of sgRNA control targeting intergenic region

    Depositor

    William Hahn

    Article

    So et al JCI Insight. 2022 Oct 10;7(19):e158755.

    Insert

    N/A
    

    Use

    Lentiviral

    Available Since

    June 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMS04 [myo-3p::bPGC::SL2::mCherry]

    Plasmid

    #168172

    Purpose

    Expression of bPGC in BWMs of C. elegans

    Depositor

    Alexander Gottschalk

    Article

    Woldemariam et al Genetics. 2019 Jul 22. pii: genetics.119

    Insert

    bPGC
    

    Expression

    Worm

    Available Since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. sgCTRL-hygro

    Plasmid

    #199639

    Purpose

    sgRNA control; induces CAS9 cutting in an intergenic region

    Depositor

    William Hahn

    Article

    So et al JCI Insight. 2022 Oct 10;7(19):e158755.

    Insert

    N/A
    

    Use

    Lentiviral

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTRIP-SFFV-mtagBFP-2A STING V155M 354STOP

    Plasmid

    #102595

    Purpose

    Lentivector to express STING V155M delta 354 and mTagBFP

    Depositor

    Nicolas Manel

    Article

    Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

    Insert

    STING (STING1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    V155M delta 354

    Promoter

    SFFV

    Available Since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP N1 mitoAURKA

    Plasmid

    #157754

    Purpose

    Expression of AURKA:GFP targeted to mitochondria

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    AURKA without the first 30 amino acids replaced b…

    Promoter

    CMV

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. lenti-Cas9-VQR-GFP_activity_reporter

    Plasmid

    #87156

    Purpose

    This lentiviral vector can be used to assay activity of SpCas9-VQR (NGA PAM restricted).

    Depositor

    Daniel Bauer

    Article

    Canver et al Nat Genet. 2017 Feb 20. doi: 10.1038/ng.

    Insert

    GFP and sgRNA targeting GFP (NGA restricted)
    

    Use

    Lentiviral

    Available Since

    Feb. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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