We narrowed to 6,121 results for: pcDNA 3.1

Showing: 391 - 420 of 6121 results

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pcDNA3.1-Mfn2

Plasmid

#191960

Purpose

Expression of the mitochondrial GTPase Mfn2.

Depositor

Luca Scorrano

Insert

Mfn2

Expression

Mammalian

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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CrBlue ---- pcDNA3.1

Plasmid

#62917

Purpose

CrBlue is a synthetic chimera comprising the blue opsin backbone with the intracellular loops and the C terminus of the jellyfish opsin, created to introduce spectral selectivity.

Depositor

Narasimhan Gautam

Article

Karunarathne et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

Insert

CrBlue

Expression

Mammalian

Promoter

CMV

Available Since

April 13, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-TUBG2

Plasmid

#171966

Purpose

Expression in cell lines of the TUBG2 gene

Depositor

Maria Alvarado-Kristensson

Article

Corvaisier et al Commun Biol. 2021 Jun 22;4(1):767. doi:

Insert

TUBG2 (TUBG2 Human)

Expression

Mammalian

Mutation

Removal of the internal TUBG2-EcoRI site by chang…

Available Since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-Muc2D1D2D3

Plasmid

#155215

Purpose

Expresses human MUC2 residues 21-1259

Depositor

Deborah Fass

Article

Javitt et al Cell. 2020 Oct 29;183(3):717-729.e16. do

Insert

Muc2 D1D2D3 (MUC2 Human)

Tags

6xHistadine

Expression

Mammalian

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1Xpress-BcrGAP

Plasmid

#38175

Depositor

Nora Heisterkamp

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

BCR (BCR Human)

Tags

Xpress

Expression

Mammalian

Mutation

contains GAP domain amino acids 1004-1271

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+) M-Ras(WT)

Plasmid

#224287

Purpose

Cell transfection and expression of M-Ras

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

M-Ras (MRAS Human)

Expression

Mammalian

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)_mtZFN(-)2G

Plasmid

#62799

Purpose

mtZFN2G architecture bearing (-) FokI domain

Depositor

Michal Minczuk

Article

Gammage et al EMBO Mol Med. 2014 Apr;6(4):458-66. doi:

Insert

mtZFN(-)2G

Tags

Internal FLAG tag immediately after F1B mitochond…

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)_mtZFN(+)2G

Plasmid

#62798

Purpose

mtZFN2G architecture bearing (+) FokI domain

Depositor

Michal Minczuk

Article

Gammage et al EMBO Mol Med. 2014 Apr;6(4):458-66. doi:

Insert

mtZFN(+)2G

Tags

Internal HA tag immediately after F1B mitochondri…

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mGAT1

Plasmid

#22804

Depositor

Henry Lester

Article

Mager et al J Neurosci. 1996 Sep 1. 16(17):5405-14.

Insert

pcDNA3.1-mGAT1

Expression

Mammalian

Mutation

wt

Available Since

Jan. 14, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)mGAT1YFP3

Plasmid

#41667

Depositor

Henry Lester

Article

Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

Insert

Mus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)

Tags

YFP

Expression

Mammalian

Mutation

“Monomerizing” YFP A206K mutation; 3 C-terminal-m…

Promoter

CMV

Available Since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Hygro(-)-B2-cT

Plasmid

#104355

Purpose

Expresses human beta 2 integrin tension sensor

Depositor

Pontus Nordenfelt, Timothy Springer

Article

Nordenfelt et al Nat Commun. 2016 Oct 10;7:13119. doi: 10

Insert

Human integrin b2 with tension sensor (ITGB2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1Xpress-AbrGAP

Plasmid

#38177

Depositor

Nora Heisterkamp

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

Abr GAP domain (ABR Human)

Tags

Xpress

Expression

Mammalian

Mutation

ABR GAP domain including amino acids 597-859

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-mNox1

Plasmid

#58340

Purpose

expresses mouse Nox1 in mammalian cells

Depositor

Botond Banfi, Karl-Heinz Krause

Article

Banfi et al J Biol Chem. 2003 Feb 7;278(6):3510-3. E

Insert

Mus musculus NADPH oxidase 1 alpha (Nox1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)mGAT1YFP8

Plasmid

#41669

Depositor

Henry Lester

Article

Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

Insert

Mus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)

Tags

YFP

Expression

Mammalian

Mutation

“Monomerizing” YFP A206K mutation; 8 C-terminal-m…

Promoter

CMV

Available Since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hPMCA4b

Plasmid

#47595

Depositor

Emanuel Strehler

Insert

PMCA4b (ATP2B4 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 GW-mCit-PA

Plasmid

#113449

Purpose

ProteinA-mCitrine Gateway shuttle vector for C-terminal fusions

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Type

Empty backbone

Use

Gateway shuttle vector

Tags

mCitrine-ProteinA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_RapACR_T111C_N248Q-EYFP

Plasmid

#119197

Purpose

Mammalian expression plasmid encoding fast anion channelrhodopsin mutant RapACR_T111C_N248Q

Depositor

John Spudich

Insert

Anion channelrhodopsin RapACR fast mutant T111C_N248Q

Tags

EYFP

Expression

Mammalian

Mutation

changed threonine 111 to cysteine and asparagine …

Promoter

CMV

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-VinculinI997A_TurboID_Venus

Plasmid

#200274

Purpose

Expressing actin-binding-deficient vinculin mutant with co-localization labeler TurboID and fluorescence reporter Venus

Depositor

Brenton Hoffman

Article

Tao et al Dev Cell. 2023 Mar 10:S1534-5807(23)0007

Insert

VinculinI997A_TurboID_Venus

Expression

Mammalian

Mutation

change isoleucine to alanine at 997 of vinculin

Promoter

T7

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mGnptg

Plasmid

#78106

Purpose

Mammalian expression of mouse Gnptg with C-terminal His tag

Depositor

Thomas Braulke

Article

Encarnacao et al J Biol Chem. 2011 Feb 18;286(7):5311-8.

Insert

Gnptg (Gnptg Mouse)

Tags

His

Expression

Mammalian

Promoter

CMV

Available Since

May 19, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1+-PAFAH1B1_R22-

Plasmid

#92431

Purpose

mammalian expression of PAFAH1B1 R22 mutant. The protein is truncated and non functional (negative control).

Depositor

Ralf Brandes, Matthias Leisegang

Article

Josipovic et al Acta Physiol (Oxf). 2016 Apr 28. doi: 10

Insert

PAFAH1B1 (PAFAH1B1 Human)

Expression

Mammalian

Mutation

R22 (stop codon in position 22)

Promoter

CMV

Available Since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-sapphireAKAR(T/A)

Plasmid

#118465

Purpose

Negative-control mutant for sapphireAKAR biosensor.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

sapphireAKAR(T/A)

Tags

6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Contains Thr-to-Ala mutation in substrate sequenc…

Promoter

CMV

Available Since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CHRFAM7A

Plasmid

#62284

Purpose

mammalian expression of human duplicated Alpha7 (CHRFAM7A)

Depositor

Sherry Leonard, Henry Lester

Article

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

CHRFAM7A (CHRFAM7A Human)

Expression

Mammalian

Mutation

partial duplication of CHRNA7

Promoter

CMV

Available Since

March 3, 2015

Availability

Industry, Academic Institutions, and Nonprofits
YFP B1 in pcDNA3.1

Plasmid

#36397

Depositor

Narasimhan Gautam

Article

Azpiazu et al J Biol Chem. 2004 Jun 25;279(26):27709-1

Insert

G protein B1 (GNB1 Bovine)

Tags

YFP

Expression

Mammalian

Promoter

cmv

Available Since

April 4, 2013

Availability

Academic Institutions and Nonprofits only
pCDNA3.1+ HA-hAO7

Plasmid

#137840

Purpose

N-terminal tagged human AO7 (aa 2-439) for expression in mammalian cells

Depositor

Allan Weissman

Article

Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

Insert

AO7 (RNF25 Human)

Tags

HA

Expression

Mammalian

Available Since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1(-) + SLNCR1

Plasmid

#86816

Purpose

mammalian expression of lncRNA LINC00673/SLNCR1

Depositor

Carl Novina

Article

Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

Insert

LINC00673 (LINC00673 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_UGControl

Plasmid

#133875

Purpose

Expresses UGControl in mammalian cells

Depositor

Sumio Terada

Article

Nakai et al Microscopy (Oxf). 2019 Jul 2. pii: 55252

Insert

UtrCH (UTRN Human)

Tags

flexible linker - mEGFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-ER-BSCaM2

Plasmid

#64855

Purpose

Endoplasmic reticulum (ER)-targeted version of a high-affinity FRET-based biosensor for monitoring Ca2+/CaM levels; targeted to cytosolic face of ER membrane.

Depositor

Jin Zhang

Article

Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

CYP450-BSCaM2

Tags

CFP, N-terminal 27 amino acids from CYP450, and Y…

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)_CMVtrunc_877

Plasmid

#105828

Purpose

A modified pcDNA3.1- vector with reduced expression activity of a truncated CMV promoter

Depositor

Harald Janovjak

Article

Kainrath et al Angew Chem Int Ed Engl. 2017 Apr 10;56(1

Type

Empty backbone

Expression

Mammalian

Available Since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-PM-CaNARi

Plasmid

#64850

Purpose

Plasma membrane-targeted FRET biosensor for monitoring calcineurin (CaN) activation.

Depositor

Jin Zhang

Article

Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

Lyn-CaNARi

Tags

Cerulean, N-terminal targeting sequence from Lyn …

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 PA-mCit-GW

Plasmid

#113448

Purpose

ProteinA-mCitrine Gateway shuttle vector for N-terminal fusions

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Type

Empty backbone

Use

Gateway shuttle vector

Tags

ProteinA-mCitrine

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 31, 2018

Availability

Academic Institutions and Nonprofits only

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