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Plasmid#46374DepositorInsertTvoVMA intein, inactive
UseTagsH6-GB1 and SH3ExpressionBacterialMutationN186A, T+1A mutations, inactivePromoterpBADAvailable sinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBbE8k-fcs
Plasmid#83068PurposeExpresses Fcs under pBAD regulationDepositorInsertfcs
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpBADAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pAGD335
Plasmid#219499PurposepAGD248 derivative with the retron Eco1 msr, sfGFP flanked by 2 BsaI sites , and the rest of the retron recombineering operon (Eco1 RT, CspRect, MutL-E32K) downstream of the pBad promoterDepositorInsertEco1 msr, sfGFP, Eco1 RT, CspRecT, MutLE32K
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGR
Plasmid#46002PurposeReporter plasmid for measuring terminator strength by inserting terminator of interest between the GFP and RFP, expression driven by a single PBAD promoter inducible by arabinoseDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterPBADAvailable sinceJune 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAJM.677
Plasmid#108530PurposeAraCAM + AraE + PBAD-YFP Reporter. Ara sensor for use in non-Marionette strain.DepositorInsertAraCAM + AraE + PBAD-YFP
UseTagsExpressionBacterialMutationAraC: L133F E165L E169G C280LPromoterAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_9
Plasmid#60580Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_9
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMrfp
Plasmid#59773PurposeDerived from pCM62; ampR, tetR, PBAD - rfpDepositorInsertrfp
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRnc-gGFP161
Plasmid#189536PurposeExpresses E. Coli RNase III under control of pBAD promoter and constitutively expresses gUTR-161-GFPDepositorInsertsRNase III
gUTR-161-GFP
UseTagsExpressionBacterialMutationN/APromoterJ23119 and pBADAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBbE8k-PP3359-6xHis
Plasmid#83067PurposeExpresses 6xHis tagged PP3359 under pBAD regulationDepositorInsertPP3359
UseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterpBADAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMRS-AraC-dCas9
Plasmid#220195Purposemini-Tn7 dCas9 expression vector - AraC/pBAD promoterDepositorInsertPseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
UseCRISPRTagsExpressionBacterialMutationPseudomonas aeruginosa codon optimizedPromoterAraC/pBADAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRS-AraC-GFP
Plasmid#220199Purposemini-Tn7 GFP expression vector - AraC/pBAD promoterDepositorInsertmonomeric superfolder green fluorescent protein
UseCRISPRTagsExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterAraC/pBADAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWaaD
Plasmid#210448PurposeExpress waaD under the control of PBADDepositorInsertwaaD
UseTagsExpressionBacterialMutationPromoterPBADAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWaaID
Plasmid#210449PurposeExpress waaI and waaD under the control of PBADDepositorInsertwaaI and waaD
UseTagsExpressionBacterialMutationPromoterPBADAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWaaJ
Plasmid#210450PurposeExpress waaJ under the control of PBADDepositorInsertwaaJ
UseTagsExpressionBacterialMutationPromoterPBADAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWaaK
Plasmid#210451PurposeExpress waaK under the control of PBADDepositorInsertwaaK
UseTagsExpressionBacterialMutationPromoterPBADAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWaaRK
Plasmid#210452PurposeExpress waaR and waaK under the control of PBADDepositorInsertwaaR and waaK
UseTagsExpressionBacterialMutationPromoterPBADAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWaaT
Plasmid#210453PurposeExpress waaT under the control of PBADDepositorInsertwaaT
UseTagsExpressionBacterialMutationPromoterPBADAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWaaW
Plasmid#210454PurposeExpress waaW under the control of PBADDepositorInsertwaaW
UseTagsExpressionBacterialMutationPromoterPBADAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWaaV
Plasmid#210457PurposeExpress waaV under the control of PBADDepositorInsertwaaV
UseTagsExpressionBacterialMutationPromoterPBADAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWaaVL
Plasmid#210458PurposeExpress waaV and waaL under the control of PBADDepositorInsertwaaV and waaL
UseTagsExpressionBacterialMutationPromoterPBADAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWaaX
Plasmid#210459PurposeExpress waaX under the control of PBADDepositorInsertwaaX
UseTagsExpressionBacterialMutationPromoterPBADAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRnc2-gGFP
Plasmid#189563PurposeExpresses E. Coli RNase III under control of pBAD promoter with native 5' untranslated region and constitutively expresses gUTR-129-GFPDepositorInsertsRNase III
gUTR-129-GFP
UseTagsExpressionBacterialMutationMaintains 5' UTR of rncPromoterJ23119 and pBADAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRnc-gGFP129
Plasmid#189537PurposeExpresses E. Coli RNase III under control of pBAD promoter and constitutively expresses gUTR-129-GFPDepositorInsertsRNase III
gUTR-129-GFP
UseTagsExpressionBacterialMutationN/APromoterJ23119 and pBADAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pA5c-tesA-vhb50-8fadR
Plasmid#86601Purposeexpression of tesA and Vhb50 under the control of a LacUV5 promoter and fadR under the control of a pBAD promoterDepositorInsertstesA
Vhb50
fadR
UseTagsExpressionBacterialMutationPromoterLacUV5 and pBADAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTara:500
Plasmid#60717PurposeExpresses T7 RNAP from the arabinose inducible promoter PBAD with a RBS strength of 500 from the Salis RBS calculator. Also includes a constitutive araC ORFDepositorInsertT7 RNAP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPBADAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNAS1B
Plasmid#61968PurposeDuet vector for co-expression of GFP and mCherry from PBAD and PLtetO promoters, respectively; parent vector for split mCherry assembly vectorsDepositorInsertsmCherry
GFP
UseTagsExpressionBacterialMutationSerine 65 to Glycine (GGT), Serine 72 to Alanine …PromoterPBAD and PLtetOAvailable sinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_2
Plasmid#60574Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_2
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_7
Plasmid#60578Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_7
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_5
Plasmid#60577Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_5
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_8
Plasmid#60579Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_8
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_12
Plasmid#60583Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_12
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_13
Plasmid#60584Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_13
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_3
Plasmid#60575Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_3
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_10
Plasmid#60581Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_10
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable sinceMarch 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_11
Plasmid#60582Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_11
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_4
Plasmid#60576Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_4
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBW300ParsR-Amp32T
Plasmid#78652PurposepSB3K3 carrying araC-PBAD-30hrpV-t-J101-32arsR-t-ParsR-30hrpR-32hrpS-t-hrpL-30gfp-tDepositorInsertaraC-PBAD-30hrpV-t-J101-32arsR-t-ParsR-
UseSynthetic BiologyTagsExpressionMutationPromotersee insertAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pWaaTW-R1
Plasmid#210455PurposeExpress waaT and waaW from E. coli R1 under the control of PBADDepositorInsertwaaT and waaW
UseTagsExpressionBacterialMutationPromoterPBADAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWaaTW-R4
Plasmid#210456PurposeExpress waaT and waaW from E. coli R4 under the control of PBADDepositorInsertwaaT and waaW
UseTagsExpressionBacterialMutationPromoterPBADAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBW301ParsR-Amp30T
Plasmid#78653PurposepSB3K3 carrying araC-PBAD-30hrpV-t-J101-32arsR-t-ParsR-30hrpR-30hrpS-t-hrpL-30gfp-tDepositorInsertaraC-PBAD-30hrpV-t-J101-32arsR-t-ParsR-
UseSynthetic BiologyTagsExpressionMutationPromotersee insertAvailable sinceAug. 1, 2016AvailabilityAcademic Institutions and Nonprofits only