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Plasmid#41608DepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTags3xHA, A. gossypii translation elongation factor 1…ExpressionMutationPromoterAvailable sinceDec. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUTKan-3xHA-GFP-TOM5
Plasmid#130674PurposeUbiquitously expresses 3xHA-GFP-TOM5 in the outer mitochondrial membrane of plantaDepositorInsertTranslocase of outer mitochondrial membrane 5 (TOM5 Mustard Weed)
UseTags3xHA and sGFPExpressionPlantMutationPromoterUbiquitin10Available sinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mTID-HA3-KanMX6
Plasmid#227982PurposeTo insert biotin ligase, miniTurboID, at the C-terminus of genes; yeast BioID, KanMX6 markerDepositorInsertminiTurboID
UseTagsHA tagExpressionYeastMutationPromoterAvailable sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX-tetO-Pcyc1
Plasmid#41023DepositorInserttetO7-Pcyc1
UsePromoter replacementTagsExpressionMutationPromoterAvailable sinceDec. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pKanCMV-mClover3-18aa-actin
Plasmid#74259PurposeMammalian expression of N-terminal fusion of mClover3 to actinDepositorInsertActin
UseTagsmClover3ExpressionMammalianMutationPromoterCAG and CMVAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKan-PCUP1-AID*(N)-9myc
Plasmid#99526PurposeN-terminal AID*-9myc degron cassette with CUP1 promoter and KanR marker for S. cerevisiaeDepositorInsertIAA17(71-116) (AXR3 Mustard Weed)
UsePcr template for yeast tagging cassetteTags9xMycExpressionMutationPromoterCUP1Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKanCMV-mRuby3-18aa-actin
Plasmid#74255PurposeMammalian expression of N-terminal fusion of mRuby3 to actinDepositorInsertactin
UseTagsmRuby3ExpressionMammalianMutationPromoterCAG and CMVAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKanCMV-mClover3-18aa-Tubulin
Plasmid#74253PurposeMammalian expression of N-terminal fusion of mClover3 to TubulinDepositorInsertTubulin
UseTagsmClover3ExpressionMammalianMutationPromoterCAG and CMVAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
p3E-FRT-KanR-FRT pA
Plasmid#82601Purpose3' entry vector containing FRT-flanked kanamycin resistance cassette (KanR) with pA for FLP-induced kanamycin resistance, drug selectionDepositorInsertFRT-Kan-FRT
UseTagsExpressionMutationPromoterNoneAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomApple-Kan
Plasmid#44957PurposeYeast tagging vector to create gene fusion with yeast optimized mApple with Kan selectionDepositorInsertyomApple
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoTagRFP657-Kan
Plasmid#44955PurposeYeast tagging vector to create gene fusion with yeast optimized TagRFp657 with Kan selectionDepositorInsertyoTagRFP657
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUC19-link-mVenus-FLAG:Kan
Plasmid#211830PurposePlasmid template that encodes the fluorescent protein mVenus with a FLAG tag next to kanamycin resistance gene. This template was used to copy out mVenus-FLAG-Kan for genetic tagging.DepositorInsertmVenus-FLAG-Kan
UseTagsExpressionBacterialMutationNAPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-Purg1-3HA
Plasmid#39351DepositorUseYeast genomic targetingTags3xHA and kanMX6 module - reverse orientationExpressionMutationPromoterAvailable sinceJan. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-PGAL1-GST
Plasmid#41611DepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionMutationPromoterAvailable sinceDec. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomEos2-Kan
Plasmid#44944PurposeYeast tagging vector to create gene fusion with yeast optimized mEos2 with Kan selectionDepositorInsertyomEos2
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomKeima-Kan
Plasmid#44902PurposeYeast tagging vector to create gene fusion with yeast optimized mKeima with Kan selectionDepositorInsertyomKeima
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P3nmt1-3HA
Plasmid#39283DepositorInsertnmt1 promoter (nmt1 Fission Yeast)
UseYeast genomic targetingTags3xHA, A. gossypii translation elongation factor 1…ExpressionMutation-1163 to +6 of nmt1 locusPromoterAvailable sinceAug. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-PGAL1-GFP
Plasmid#41614DepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionMutationPromoterAvailable sinceDec. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoPSmOrange-Kan
Plasmid#44951PurposeYeast tagging vector to create gene fusion with yeast optimized photoconvertible mOrange with Kan selectionDepositorInsertyoPSmOrange
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA CUP1p-NuiHA kanMX
Plasmid#131169PurposePCR template for genomic ScCUP1 promoter regulated N-terminal Nui-HA prey fusion, kanMX marker confers resistance to geneticin in S. cerevisiaeDepositorTypeEmpty backboneUseTagsNui-HAExpressionYeastMutationPromoterAvailable sinceDec. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKanCMV-CX4.3-7aa-mClover3
Plasmid#74260PurposeMammalian expression of C-terminal fusion of mClover3 to CX43DepositorInsertConnexin 43
UseTagsmClover3ExpressionMammalianMutationPromoterCAG and CMVAvailable sinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGZ173 (pFA6a-SL-MNase-kanMX6)
Plasmid#70234PurposeChEC-tagging vector encoding a short linker and aa 83-231 of Mnase in pFA6a-3HA-kanMX6 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitopeDepositorInsertShort linker and aa 83-231 of Mnase
UseYeast genomic targetingTagsExpressionMutationPromoterAvailable sinceNov. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDZ251 (pKAN 24xPP7SL cyc)
Plasmid#35192DepositorInsert24xPP7SL cyc
UseTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoClover-Kan
Plasmid#44952PurposeYeast tagging vector to create gene fusion with yeast optimized Clover with Kan selectionDepositorInsertyoClover
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUC-H1-Esp3I-mU6-Kan
Plasmid#213015PurposeVector for Cloning of multiple gRNAs driven by distinct promoters (H1 and mU6)DepositorInsertH1 promoter, mU6 promoter, gRNA scaffolds
UseTagsExpressionMutationPromoterH1 promoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P41nmt1-GFP
Plasmid#39290DepositorInsertnmt1 promoter (nmt1 Fission Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionMutation-1163 to +6 of nmt1 locus with a 4bp deletion (TA…PromoterAvailable sinceAug. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pETNKI-his-3C-LIC-kan
Plasmid#108703PurposeExpression of an His-tagged target protein with 3C protease cleavage site.DepositorTypeEmpty backboneUseTagsHis-3C protease cleavage siteExpressionBacterialMutationPromoterT7Available sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-G11-5FLAG-kanMX6
Plasmid#49182Purposegenomic G11-5FLAG epitope tagging in S. pombeDepositorTypeEmpty backboneUseTagsG11-5FLAGExpressionYeastMutationPromoterAvailable sinceNov. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomWasabi-Kan
Plasmid#44945PurposeYeast tagging vector to create gene fusion with yeast optimized mWasabi with Kan selectionDepositorInsertyomWasabi
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUC19-link-sfGFP-FLAG:Kan
Plasmid#211833PurposePlasmid template that encodes the fluorescent protein sfGFP with a FLAG tag next to kanamycin resistance gene. This template was used to copy out sfGFP-FLAG-Kan for genetic tagging.DepositorInsertsfGFP-FLAG-Kan
UseTagsExpressionBacterialMutationNAPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19-link-PAGFP-FLAG:Kan
Plasmid#211834PurposePlasmid template that encodes the fluorescent protein PAGFP with a FLAG tag next to kanamycin resistance gene. This template was used to copy out PAGFP-FLAG-Kan for genetic tagging.DepositorInsertPAGFP-FLAG-Kan
UseTagsExpressionBacterialMutationNAPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P81nmt1-mYFP
Plasmid#105144PurposeYeast gene targetingDepositorInsertkanMX6-P81nmt1-mYFP
UseTags81nmt1-mYFPExpressionBacterial and YeastMutationA206KPromoter81nmt1Available sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomKO2-Kan
Plasmid#44958PurposeYeast tagging vector to create gene fusion with yeast optimized mKO2 with Kan selectionDepositorInsertyomKO2
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-6xGLY-S-tag-kanMX6
Plasmid#20766DepositorTypeEmpty backboneUsePcr-based c-terminal taggingTagsExpressionYeastMutationPromoterAvailable sinceMarch 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
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pFA6a-kanMX6-P81nmt1-GFP
Plasmid#39291DepositorInsertnmt1 promoter (nmt1 Fission Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionMutation-1163 to +6 of nmt1 locus with a 7bp deletion (AT…PromoterAvailable sinceAug. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
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BAS292 C terminal SF-GFP kanR
Plasmid#74087PurposeS.pombe recoded super-folder GFP in universal C-terminal tagging plasmid, G418 resistanceDepositorInsertSF-GFP recoded for S.pombe
UseTagsExpressionMutationPromoterAvailable sinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDZ645 pKAN 1x-mCherry-12xPP7 V4
Plasmid#73173PurposeFloxed Kanamycin (G418) selection maker; 3' tagging vector used to add 1x-mCherry followed by 12 repetitive PP7 version 4 RNA stem-loops to gene of interestDepositorInsert1x-mCherry
UseTagsExpressionMammalian and YeastMutationPromoterAvailable sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS-MCS1M(1)Kana-mCMV-TK-pA
Plasmid#22060DepositorInsertTK
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
1352wUV2 F2bbs kan GCANNNAGGk
Plasmid#18752DepositorInsert3 tandem zinc fingers
UseTagsExpressionBacterialMutationA Kan cassette (1090bp) is inserted between two b…PromoterAvailable sinceJuly 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-KanMX6-Purg1-3FLAG
Plasmid#19354DepositorInserturg1 promoter
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pETNKI-strepII-3C-LIC-kan
Plasmid#108706PurposeExpression of a StrepII-tagged target protein with 3C protease cleavage site.DepositorTypeEmpty backboneUseTagsStrepII-3C protease cleavage siteExpressionBacterialMutationPromoterT7Available sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBS-MCS1M(8)Kana-mCMV-pA
Plasmid#22059DepositorInsertmCMV-pA expression cassette
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P3nmt1-3FLAG
Plasmid#19336DepositorInsert3FLAG
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoEmerald-Kan
Plasmid#44946PurposeYeast tagging vector to create gene fusion with yeast optimized Emerald with Kan selectionDepositorInsertyoEmerald
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
1352wUV2 F3bbs kan NNNTGGGCGk
Plasmid#18756DepositorInsert3 tandem zinc fingers
UseTagsExpressionBacterialMutationA Kan cassette (1090bp) is inserted between two b…PromoterAvailable sinceJuly 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
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pSUM-Kan-MCS1-gfp
Plasmid#109288PurposeExpresses high levels of gfp in mycobacteriaDepositorInsertMultiple Cloning Site and GFP
UseTagsExpressionBacterialMutationPromoterKanamycinAvailable sinceMay 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
1352wUV2 F1bbs kan GCGTGGNNN
Plasmid#18753DepositorInsert3 tandem zinc fingers
UseTagsExpressionBacterialMutationA Kan cassette (1090bp) is inserted between two b…PromoterAvailable sinceJuly 11, 2008AvailabilityAcademic Institutions and Nonprofits only