We narrowed to 619 results for: Hc

1. Mm-PylRS-AF/Pyl-tRNACUA

   Plasmid

   #122650

   Purpose

   tRNA synthetase/tRNA pair for the incorporation of unnatural amino acid into proteins in mammalian cells in response to the amber codon TAG.

   Depositor

   Howard Hang

   Article

   Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038

   Insert

   PylRS-AF, Pyl-tRNACUA
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Tyrosine 306 changed to Alanine, Tyrosine 384 cha…

   Promoter

   Available since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6 SgRNA RBM20

   Plasmid

   #162735

   Purpose

   Mammalian expression

   Depositor

   Amit Choudhary

   Article

   Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

   Insert

   Spacer sequence for RBM20
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTub-Wg

   Plasmid

   #64321

   Purpose

   Expresses Drosophila Wingless protein under the tubulin promotor

   Depositor

   Roel Nusse

   Article

   Ching et al J Biol Chem. 2008 Jun 20;283(25):17092-8

   Insert

   Wingless (wg Fly)
   

   Use

   Drosophila expression

   Tags

   Expression

   Mutation

   Promoter

   alpha-1 tubulin promoter

   Available since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pCFJ150-mCherry(dpiRNA)::ANI-1(AHPH)

   Plasmid

   #107939

   Purpose

   pie-1p::mCherry(dpiRNA)::ANI-1(AHPH)::pie-1 3'UTR construct used for MosSCI in nematode. mCherry is optimized by removing all piRNA targeting sites to allow germline expression. RhoA biosenser.

   Depositor

   Heng-Chi Lee

   Article

   Zhang et al Science. 2018 Feb 2;359(6375):587-592. d

   Insert

   mCherry(dpiRNA)::ANI-1(AHPH)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   pie-1

   Available since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFP-Trak2

   Plasmid

   #127622

   Purpose

   N-terminal GFP-tagged mouse Trak2

   Depositor

   Josef Kittler

   Article

   Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

   Insert

   Trak2 (Trak2 Mouse)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCIG PIK3CA E545K

   Plasmid

   #73055

   Purpose

   Human PIK3CA E545K

   Depositor

   Joseph Gleeson

   Article

   Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

   Insert

   PIK3CA (PIK3CA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E545K

   Promoter

   Available since

   March 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHAGE DN Cullin3

   Plasmid

   #41913

   Depositor

   Stephen Elledge

   Article

   Emanuele et al Cell. 2011 Oct 14;147(2):459-74. doi: 10

   Insert

   Cullin 3 (CUL3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHAGE DN Cullin4B

   Plasmid

   #41915

   Depositor

   Stephen Elledge

   Article

   Emanuele et al Cell. 2011 Oct 14;147(2):459-74. doi: 10

   Insert

   CUL4B (CUL4B Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GFP-Trak1

   Plasmid

   #127621

   Purpose

   N-terminal GFP-tagged mouse Trak1

   Depositor

   Josef Kittler

   Article

   Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

   Insert

   Trak1 (Trak1 Mouse)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCIG PIK3CA Wildtype

    Plasmid

    #73056

    Purpose

    Human PIK3CA Wildtype

    Depositor

    Joseph Gleeson

    Article

    Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

    Insert

    PIK3CA Wildtype (PIK3CA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCFJ150-GFP(dpiRNA)::CDK-1

    Plasmid

    #107938

    Purpose

    cdk-1p::GFP(dpiRNA)::cdk-1::cdk-1 3'UTR construct used for MosSCI in nematode. GFP is optimized by removing all piRNA targeting sites to allow germline expression.

    Depositor

    Heng-Chi Lee

    Article

    Zhang et al Science. 2018 Feb 2;359(6375):587-592. d

    Insert

    GFP(dpiRNA)::CDK-1 (cdk-1 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    cdk-1

    Available since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1-Myc-His RTN3A1

    Plasmid

    #31087

    Depositor

    Jimtong Horng

    Article

    Tang et al J Biol Chem. 2007 Feb 23. 282(8):5888-98

    Insert

    reticulon 3 (RTN3 Human)
    

    Use

    Tags

    Myc-His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMJA167

    Plasmid

    #128536

    Purpose

    Expresses DsRed

    Depositor

    Marcos Alcocer

    Article

    Wang et al Mol Biotechnol. 2014 Feb;56(2):136-46. d

    Insert

    NFAT-DsRed reporter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40-NFAT

    Available since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCAGGS-FLAG-CaMKIID-K43M

    Plasmid

    #186830

    Purpose

    Expresses FLAG-CaMKIID-K43M in mammalian cells

    Depositor

    Hung-Chun Chang

    Article

    Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

    Insert

    Camk2d (Camk2d Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Lysine 43 to Methionine

    Promoter

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pU6 SgRNA GAPDH

    Plasmid

    #162736

    Purpose

    Mammalian expression

    Depositor

    Amit Choudhary

    Article

    Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

    Insert

    Spacer sequence for GAPDH
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET SUMO NL-FKBP-Cas9

    Plasmid

    #162730

    Purpose

    Bacterial expression, protein expression

    Depositor

    Amit Choudhary

    Article

    Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

    Insert

    FKBP F36V
    

    Use

    Tags

    6xHis and SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAGGS-FLAG-BMAL1-S513A

    Plasmid

    #186831

    Purpose

    Expresses FLAG-BMAL1-S513A in mammalian cells

    Depositor

    Hung-Chun Chang

    Article

    Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

    Insert

    bmal1 (Bmal1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Serine 513 to Alanine

    Promoter

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAGGS-FLAG-BMAL1-S515A

    Plasmid

    #186832

    Purpose

    Expresses FLAG-BMAL1-S515A in mammalian cells

    Depositor

    Hung-Chun Chang

    Article

    Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

    Insert

    bmal1 (Bmal1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Serine 515 to Alanine

    Promoter

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAGGS-FLAG-BMAL1-S516A

    Plasmid

    #186833

    Purpose

    Expresses FLAG-BMAL1-S516A in mammalian cells

    Depositor

    Hung-Chun Chang

    Article

    Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

    Insert

    bmal1 (Bmal1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Serine 516 to Alanine

    Promoter

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAGGS-FLAG-BMAL1-S519A

    Plasmid

    #186834

    Purpose

    Expresses FLAG-BMAL1-S519A in mammalian cells

    Depositor

    Hung-Chun Chang

    Article

    Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

    Insert

    bmal1 (Bmal1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Serine 519 to Alanine

    Promoter

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge