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pAAV.CMV.SV40.THBS1-HA.SV40(polyA)

Plasmid

#154850

Purpose

Expresses murine Thrombospondin-1 (THBS1) protein with HA-tag at C-terminus

Depositor

Kevin Park

Article

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

Thrombospondin-1 (Thbs1 Mouse)

Use

AAV

Tags

HA-tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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mAC-POLR2A donor (Hygro)

Plasmid

#124496

Purpose

A donor plasmid for tagging the N-terminus of human POLR2A with mAID-mClover

Depositor

Masato Kanemaki

Article

Nagashima et al J Cell Biol. 2019 Mar 1. pii: jcb.201811

Insert

POLR2A homology arms and Hygro-P2A-mAID-mClover (POLR2A Human)

Use

CRISPR

Tags

mAID-mClover

Expression

Mutation

Promoter

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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AP1muA-SNAP-V5-polyA-Puro HR

Plasmid

#229680

Purpose

Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with SNAP tag and a V5 epitope tag. Contains a puromycin resistance cassette for selection of edited cells.

Depositor

Francesca Bottanelli

Article

Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

Insert

genomic sequence AP1M1 locus (AP1M1 Human)

Use

CRISPR

Tags

SNAP, V5

Expression

Mutation

Promoter

Available since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.CMV.SV40.THBS1-HA-KDEL.SV40(polyA)

Plasmid

#156164

Purpose

Expresses murine Thrombospondin-1 (THBS1) protein with HA-tag at C-terminus and KDEL (ER retention) sequence

Depositor

Kevin Park

Article

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

Thrombospondin-1 (Thbs1 Mouse)

Use

AAV

Tags

HA-tag

Expression

Mammalian

Mutation

THBS1 with addition of ER-retention sequence KDEL

Promoter

CMV

Available since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-H3.3-polyA

Plasmid

#59780

Purpose

Express Flag-tagged H3.3 (H3F3B) in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

H3F3B (H3-3B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV.CMV.SV40.THBS4-HA.SV40(polyA)

Plasmid

#155193

Purpose

Expresses murine Thrombospondin-4 (THBS4) protein with HA-tag at C-terminus

Depositor

Kevin Park

Article

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

Thrombospondin-4 (Thbs4 Mouse)

Use

AAV

Tags

HA-tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pLL3.7-EF-EYFP-YAP1_WT-PolyA

Plasmid

#112284

Purpose

Fluorescently labels WT human YAP1-2gamma isoform (504 aa)

Depositor

Erik Sahai

Article

Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

Insert

EYFP-YAP1 (YAP1 Human)

Use

Lentiviral

Tags

Expression

Mutation

WT human YAP1-2gamma

Promoter

EF-1 alpha

Available since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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hSyn-DIO-somBiPOLES-mCerulean

Plasmid

#154951

Purpose

Optogenetic tool for blue-light inhibition and red-light excitation of neurons

Depositor

Simon Wiegert

Article

Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

Has Service

AAV Retrograde, AAV5, and AAV9

Insert

somBiPOLES

Use

AAV

Tags

soma-targeting motif from Kv2.1 channel

Expression

Mammalian

Mutation

Promoter

human synapsin

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pRS405-LEU2-TEF-KpolDcr1v2

Plasmid

#85193

Purpose

Integrating plasmid, K. polysporus Dcr1 under TEF promoter

Depositor

David Bartel

Article

Nakanishi et al Nature. 2012 Jun 20;486(7403):368-74. do

Insert

Dcr1

Use

Integrating, shuttle vector

Tags

Expression

Yeast

Mutation

Promoter

TEF

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast POLE1

Plasmid

#160802

Purpose

Express POLE1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

POLE1 (POLE Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mutation

shPOLE_1 and shPOLE2 resistant

Promoter

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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Wild-type AdPol vector

Plasmid

#131685

Purpose

A lentiviral vector that constitutively expresses the wild-type adenovirus DNA polymerase

Depositor

Matthew D. Shoulders

Article

Berman et al J Am Chem Soc. 2018 Dec 26;140(51):18093

Insert

Adenovirus DNA polymerase

Use

Lentiviral

Tags

HA

Expression

Mutation

Promoter

Available since

June 27, 2020

Availability

Academic Institutions and Nonprofits only

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attB-GFP-Dnmt3b1-Poly(A)

Plasmid

#65531

Purpose

Plasmid for Bxb1-mediated recombination of the GFP-Dnmt3b1 cDNA into a MIN-tagged locus

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

Dnmt3b (Dnmt3b Mouse)

Use

Mouse Targeting; Bxb1

Tags

GFP

Expression

Mammalian

Mutation

isoform 1

Promoter

Available since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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Renilla luciferase-Pol III

Plasmid

#37380

Depositor

Norbert Perrimon

Article

Nybakken et al Nat Genet. 2005 Dec;37(12):1323-32. Epub

Insert

PolIII-Renilla control reporter (RpIII128 Fly)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

RNA PolIII 128 subunit (RpIII128)

Available since

July 30, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA-flag-hKDM4A-polyA

Plasmid

#101051

Purpose

in vitro transcription (T7 promoter)

Depositor

Yi Zhang

Article

Chung et al Cell Stem Cell. 2015 Dec 3;17(6):758-66.

Insert

KDM4A (KDM4A Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

T7

Available since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-flag-mKdm4d-polyA

Plasmid

#61553

Purpose

For mRNA synthesis or expression in mammalian cells

Depositor

Yi Zhang

Article

Matoba et al Cell. 2014 Nov 6;159(4):884-95. doi: 10.

Insert

Kdm4d (Kdm4d Mouse)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA-RanWT-mRFP1-polyA

Plasmid

#59750

Purpose

Express wild-type form of Ran-mRFP1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

Ran-mRFP1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only

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AICSDP-117:POLR2A-mEGFP

Plasmid

#164500

Purpose

Homology arms and mEGFP-linker sequence sequence for N-terminus tagging of human POLR2A

Depositor

Allen Institute for Cell Science

Insert

POLR2A Homology Arms with mEGFP-linker (POLR2A Human)

Use

CRISPR; Donor template

Tags

mEGFP-linker

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Promoter

Available since

Feb. 17, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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Ef1a-DIO-BiPOLES-mCerulean

Plasmid

#154949

Purpose

Optogenetic tool for blue-light inhibition and red-light excitation of neurons

Depositor

Simon Wiegert

Article

Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

Insert

BiPOLES

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Ef1-alpha

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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KK601: pMVP (L3-L2) ecDHFR DD + polyA

Plasmid

#121790

Purpose

pMVP L3-L2 entry plasmid, contains ecDHFR DD + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term ecDHFR degron domain (stabilized by TMP) to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

ecDHFR Degron Domain + polyA

Use

Synthetic Biology; Pmvp gateway entry plasmid

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-Nup37-EGFP-polyA

Plasmid

#104562

Purpose

Express Nup37-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

Nup37 (Nup37 Mouse)

Use

Tags

EGFP tag

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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