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pFS_0358_pET30-RT-CRISPR_Micromonospora_rosaria_ARRAY_1_RC

Plasmid

#116970

Purpose

Expresses MrRT-Cas1-Cas2 under pT7lac promoter, encodes MrCRISPR array 1 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Micromonospora rosaria RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0359_pET30-RT-CRISPR_Micromonospora_rosaria_ARRAY_2

Plasmid

#116971

Purpose

Expresses MrRT-Cas1-Cas2 under pT7lac promoter, encodes MrCRISPR array 2, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Micromonospora rosaria RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0361_pET30-RT-CRISPR_Micromonospora_rosaria_ARRAY_3

Plasmid

#116973

Purpose

Expresses MrRT-Cas1-Cas2 under pT7lac promoter, encodes MrCRISPR array 3, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Micromonospora rosaria RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0360_pET30-RT-CRISPR_Micromonospora_rosaria_ARRAY_2_RC

Plasmid

#116972

Purpose

Expresses MrRT-Cas1-Cas2 under pT7lac promoter, encodes MrCRISPR array 2 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Micromonospora rosaria RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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TRE reporter backbone

Plasmid

#158678

Purpose

This plasmid serves as the backbone for the TRE reporters described in our paper. It's a promoterless-mStrawberry-pGK-BSD backbone to which we insert a pathway specific promoter before the mStrawberry

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Type

Empty backbone

Use

Lentiviral

Tags

mStrawberry

Expression

Mammalian

Mutation

Promoter

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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CFP_ betaGlobin_24XPP7_24XMS2_Splicing_ reporter

Plasmid

#61762

Purpose

Dual Color betaGlobin reporter to study splicing kinetics in vivo

Depositor

Daniel Larson

Article

Coulon et al Elife. 2014 Oct 1;3. doi: 10.7554/eLife.

Insert

b-Globin

Use

Tags

24 X MS2 hairpin cassette in 3'UTR of b-Glob…

Expression

Mammalian

Mutation

Promoter

Dox inducible Tet-ON promoter

Available since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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eGFP L202 Reporter

Plasmid

#119129

Purpose

Acts as a reporter for APOBEC-medated single-base editing. mCherry acts as a transfection control and eGFP as a reporter.

Depositor

Reuben Harris

Article

Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

Insert

mCherry and eGFP

Use

Lentiviral

Tags

Expression

Mutation

eGFP L202S

Promoter

CMV

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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mPiezo2-CMV-Sport6

Plasmid

#81073

Purpose

mouse Piezo2

Depositor

Ardem Patapoutian

Article

Coste et al Nat Commun. 2015 May 26;6:7223. doi: 10.

Insert

Piezo2 (please see depositor comments below) (Piezo2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Actb KI

Plasmid

#131479

Purpose

Endogenous tagging of βactin: N-terminal (amino acid position: D2)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Actb, Actb Mouse, Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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4XCLEAR-luciferase reporter

Plasmid

#66800

Purpose

Reporter for activity of the CLEAR network

Depositor

Albert La Spada

Article

Cortes et al Nat Neurosci. 2014 Sep;17(9):1180-9. doi

Insert

4XCLEAR

Use

Luciferase

Tags

Expression

Mutation

Promoter

Four CLEAR elements (GTCACGTGAC) in tandem derive…

Available since

Nov. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pSV Sport SREBP-1c

Plasmid

#8883

Depositor

Bruce Spiegelman

Article

Tontonoz et al Mol Cell Biol. 1993 Aug . 13(8):4753-9.

Insert

SREBP-1c (Srebf1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Active version of SREBP-1c.

Promoter

Available since

July 18, 2006

Availability

Academic Institutions and Nonprofits only

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CRISPR-SP-Cas9 reporter

Plasmid

#62733

Purpose

Mammalian CRISPR-SP-Cas9 reporter

Depositor

Niels Geijsen

Article

D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

Insert

CRISPR-SP-Cas9 target seq + tdTomato (out of frame)

Use

CRISPR and Lentiviral

Tags

CRISPR-SP-Cas9 target seq (hAAVS1 locus) and HA t…

Expression

Mammalian

Mutation

Promoter

EF1-Alpha

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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GAL4UAS-Luciferase reporter

Plasmid

#64125

Purpose

Photoactivatable transcription system. Luciferase reporter under the control of the upstream activator sequence (UAS) of Gal4.

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

GAL4UAS

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

GAL4UAS

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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NFAT luciferase reporter

Plasmid

#10959

Depositor

Toren Finkel

Article

Ichida et al J Biol Chem. 2001 Feb 2. 276(5):3524-30.

Insert

IL-2 promoter (IL2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2006

Availability

Academic Institutions and Nonprofits only

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pLV-REPORT(EF1a)

Plasmid

#172326

Purpose

LV REPORT containing minimal promoter driving monomeric nuclear Tomato followed by an EF1a promoter driving nuclear d2eGFP E2A Hygromycin

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Inserts

monomeric nuclear Tomato
d2eGFP
HygR

Use

Lentiviral and Synthetic Biology

Tags

3x SV40 NLS

Expression

Mammalian

Mutation

Promoter

EF1-alpha

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Grin2b KI

Plasmid

#131487

Purpose

Endogenous tagging of GluN2b: N-terminal (amino acid position: S34)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Grin2b Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pORANGE Gria3-GFP KI

Plasmid

#131491

Purpose

Endogenous tagging of GluA3: C-terminal (amino acid position: STOP codon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Gria3, Gria3 Mouse, Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Camk2a KI

Plasmid

#131484

Purpose

Endogenous tagging of CaMKIIa: N-terminal (amino acid position: before startcodon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Camk2a, Camk2a Mouse, Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV Sport2 mPer2

Plasmid

#16204

Depositor

Cheng Lee

Article

Albrecht et al Cell. 1997 Dec 26. 91(7):1055-64.

Insert

Per2 (Per2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2007

Availability

Academic Institutions and Nonprofits only
pORANGE CACNG8-GFP KI #2

Plasmid

#131474

Purpose

Endogenous tagging of Tarpγ8: Intramolecular (amino acid position: A408)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Cacng8 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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