We narrowed to 5,994 results for: crispr cas9 expression plasmids

1. pCJ1022

   Plasmid

   #228762

   Purpose

   Plasmid expressing Cas9 and 2 gRNAs for mouse Pkd2. Use for disruption of mouse Pkd2 in cultured cells.

   Depositor

   Bradley Yoder

   Insert

   Pkd2 gRNAs (Pkd2 Mouse)
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Dec. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLdCN

   Plasmid

   #84290

   Purpose

   Express gRNA and Cas9 in Leishmania with Neomycin resistance

   Depositor

   Greg Matlashewski

   Article

   Zhang et al mSphere. 2017 Jan 18;2(1). pii: e00340-1

   Insert

   gRNA and Cas9
   

   Use

   CRISPR; Leishmania

   Tags

   Expression

   Mutation

   Promoter

   L. donovani ribosome RNA promoter

   Available since

   Jan. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLdCH

   Plasmid

   #84291

   Purpose

   Express gRNA and Cas9 in Leishmania with Hygromycin resistance

   Depositor

   Greg Matlashewski

   Article

   Zhang et al mSphere. 2017 Jan 18;2(1). pii: e00340-1

   Insert

   gRNA and Cas9
   

   Use

   CRISPR; Leishmania

   Tags

   Expression

   Mutation

   Promoter

   L. donovani ribosome RNA promoter

   Available since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-dSa-VP64

   Plasmid

   #99680

   Purpose

   Expresses dSa Cas9 fused to gold standard VP64 activator

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9
   

   Use

   AAV

   Tags

   VP64

   Expression

   Mutation

   dead Cas9

   Promoter

   Available since

   Oct. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. BPK2660

   Plasmid

   #70709

   Purpose

   Human expression plasmid for SaCas9 sgRNA(84nt in length) (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sa-sgRNA(84)

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKLV2-U6(BsmBI)-EF1a-BFP-BSD

   Plasmid

   #117136

   Purpose

   Empty vector encoding BFP-BSD

   Depositor

   Kosuke Yusa

   Insert

   BSD2A
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   modified BFP

   Promoter

   human U6 promoter, human EF1a promoter

   Available since

   April 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. iCas

   Plasmid

   #84232

   Purpose

   Expression of SpCas9 with 4 ERT2 fusion protein and empty gRNA cassette. The activity of Cas9 can be switched on and off in human cells with 4-hydroxytamoxifen (4-HT)

   Depositor

   Meng How Tan

   Article

   Liu et al Nat Chem Biol. 2016 Sep 12. doi: 10.1038

   Insert

   Cas9
   

   Use

   Tags

   2A-OFP co-expression and ERT2-ERT2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSc1

   Plasmid

   #80436

   Purpose

   Expresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cell

   Depositor

   Ervin Welker

   Article

   Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare

   Inserts

   sp Cas9 gRNA
   eGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   Aug. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330-Cetn1/1

   Plasmid

   #50718

   Purpose

   pX330 containing sgRNA against mouse Cent1. Positive control for DSB mediated EGFP reconstitution.

   Depositor

   Masahito Ikawa

   Article

   Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

   Inserts

   Cetn1 sgRNA1
   humanized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and hU6

   Available since

   Feb. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pVT36b

    Plasmid

    #184437

    Purpose

    CRISPR/Cas9 plasmid, containing ScARS, IoURA3, iCas9, RPR1’-tRNA_Leu promoter, and sgRNA scaffold

    Depositor

    Huimin Zhao

    Article

    Tran et al mSphere. 2019 Jun 26;4(3). pii: 4/3/e003

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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