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We narrowed to 4,580 results for: NAP

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Showing: 4171 - 4200 of 4580 results

  2. IBMc397

    Plasmid
    #161955
    Purpose
    Demonstrates chemically induced dimmerization of acVHH. Expression plasmid for split, evolved T7 RNA polymerase C-lobe fused to acVHH
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB3K3-TT-PBAD(SapI-)-B30-His-acVHH-15aa-T7RNAP(181+)-T
    Expression
    Bacterial
    Promoter
    PBAD
    Available Since
    Oct. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    161955-plasmid-map-sequence-id-326361

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  5. pVITRO-Fab_Theo-LUCID

    Plasmid
    #119048
    Purpose
    Plasmid encoding the mammalian expression of the Theophiline LUCID based on antibodies (BRET semi-synthetic biosensor)
    Depositor
    Kai Johnsson
    Article
    Xue et al Angew Chem Int Ed Engl. 2017 Jun 12;56(2
    Inserts
    Fab_Theo-LUCID_part1
    Fab_Theo-LUCID_part2
    Tags
    FLAG-tag, His-tag, and Strep-tag
    Expression
    Mammalian
    Promoter
    EF-1α + CMV/SV40 enhancers and IRES
    Available Since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    119048-plasmid-map-sequence-id-231811

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  6. pVITRO-Fab_Quin-LUCID

    Plasmid
    #119049
    Purpose
    Plasmid encoding the mammalian expression of the Quinine LUCID based on antibodies (BRET semi-synthetic biosensor)
    Depositor
    Kai Johnsson
    Article
    Xue et al Angew Chem Int Ed Engl. 2017 Jun 12;56(2
    Inserts
    Fab_Quin-LUCID_part1
    Fab_Quin-LUCID_part2
    Tags
    FLAG-tag, His-tag, and Strep-tag
    Expression
    Mammalian
    Promoter
    EF-1α + CMV/SV40 enhancers and IRES
    Available Since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    119049-plasmid-map-sequence-id-231810

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  7. pVITRO-Fab_MTX-LUCID

    Plasmid
    #119047
    Purpose
    Plasmid encoding the mammalian expression of the Metotraxate LUCID based on antibodies (BRET semi-synthetic biosensor)
    Depositor
    Kai Johnsson
    Article
    Xue et al Angew Chem Int Ed Engl. 2017 Jun 12;56(2
    Inserts
    Fab_MTX-LUCID_part1
    Fab_MTX-LUCID_part2
    Tags
    FLAG-tag, His-tag, and Strep-tag
    Expression
    Mammalian
    Promoter
    EF-1α + CMV/SV40 enhancers and IRES
    Available Since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    119047-plasmid-map-sequence-id-231808

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  8. pBAD-MTX-u36_Snifit

    Plasmid
    #119045
    Purpose
    Plasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 36 (FRET semi-synthetic biosensor)
    Depositor
    Kai Johnsson
    Article
    Xue et al J Am Chem Soc. 2016 Apr 27;138(16):5258-
    Insert
    MTX-u36_Snifit
    Tags
    His-tag and Strep-tag
    Expression
    Bacterial
    Mutation
    36TAG R44L H45Q
    Promoter
    pBAD
    Available Since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    119045-plasmid-map-sequence-id-229505

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  9. pBAD-MTX-u51_Snifit

    Plasmid
    #119046
    Purpose
    Plasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 51 (FRET semi-synthetic biosensor)
    Depositor
    Kai Johnsson
    Article
    Xue et al J Am Chem Soc. 2016 Apr 27;138(16):5258-
    Insert
    MTX-u51_Snifit
    Tags
    His-tag and Strep-tag
    Expression
    Bacterial
    Mutation
    51TAG R44L H45Q
    Promoter
    pBAD
    Available Since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    119046-plasmid-map-sequence-id-229503

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  10. pBAD-MTX-u23_Snifit

    Plasmid
    #119044
    Purpose
    Plasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 23 (FRET semi-synthetic biosensor)
    Depositor
    Kai Johnsson
    Article
    Xue et al J Am Chem Soc. 2016 Apr 27;138(16):5258-
    Insert
    MTX-u23_Snifit
    Tags
    His-tag and Strep-tag
    Expression
    Bacterial
    Mutation
    23TAG R44L H45Q
    Promoter
    pBAD
    Available Since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    119044-plasmid-map-sequence-id-229496

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  12. PX552

    Plasmid
    #60958
    Purpose
    pAAV-U6sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). AAV plasmid for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei.
    Depositor
    Feng Zhang
    Article
    Swiech et al Nat Biotechnol. 2014 Oct 19. doi: 10.103
    Inserts
    U6_(SpaI)_sgRNA
    Syn_EGFP-KASH
    Use
    AAV, CRISPR, and Mouse Targeting
    Expression
    Mammalian
    Promoter
    Synapsin and U6
    Available Since
    Nov. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    60958-plasmid-map-sequence-id-382829

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  16. pclbw-eGFPomp25

    Plasmid
    #58424
    Purpose
    Mammalian expression of GFP targeted to the mitochondrial outer membrane
    Depositor
    David Chan
    Article
    Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi
    Insert
    eGFP-omp25 (Synj2bp Mouse)
    Use
    Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    contains aa's 109-145 of mouse omp25
    Promoter
    CMV/b-actin
    Available Since
    Dec. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  19. pEVOL-NBK-1

    Plasmid
    #207639
    Purpose
    A plasmid for the expression of M. mazei pyrrolysine aminoacyl-tRNA synthetase/tRNA pair (PylRS/tRNAPyl) in E. Coli.
    Depositor
    Cole DeForest
    Article
    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1
    Insert
    Pyl-tRNACUA and 2x pyrrolysyl-tRNA synthetase (MmPylRS)
    Expression
    Bacterial
    Mutation
    The evolved synthetase NBK-1 contains mutations Y…
    Promoter
    araBAD
    Available Since
    Oct. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    207639-plasmid-map-sequence-id-409890

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  28. pAAV-hSyn-STChRger2-TS-EYFP

    Plasmid
    #129395
    Purpose
    Soma Targeted, High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter.
    Depositor
    Viviana Gradinaru
    Article
    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s
    Insert
    soma targeted ChRger2
    Use
    AAV
    Tags
    Kv2.1-TS-EYFP
    Expression
    Mammalian
    Promoter
    hSyn
    Available Since
    Oct. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    129395-plasmid-map-sequence-id-258248

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  29. pAAV-hsyn-DIO-Rpl22l1-3Flag-2A-eGFP-WPRE

    Plasmid
    #214265
    Purpose
    Expression of a Flag-tagged Rpl22I1 protein in cells expressing Cre recombinase allowing for the harvesting of actively transcribed mRNAs in that cell type.
    Depositor
    D. James Surmeier
    Insert
    Flag-tagged Rpl22I1 protein expressing Cre Recombinase (Rpl22 Mouse)
    Use
    AAV
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    Synapsin
    Available Since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    214265-plasmid-map-sequence-id-429396

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  30. pCS6-YFP-SMASh(AI)

    Plasmid
    #111500
    Purpose
    Expresses Venus YFP with a C-terminal SMASh(AI) tag fusion in mammalian cells. [AI = asunaprevir inhibited].
    Depositor
    Michael Lin
    Article
    Jacobs et al Nat Methods. 2018 Jul;15(7):523-526. doi
    Insert
    YFP-SMASh(AI)
    Tags
    HA epitope tag and SMASh tag
    Expression
    Mammalian
    Mutation
    The HCV NS3 protease carries V36M, T54A, and S122…
    Promoter
    CMV
    Available Since
    July 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    111500-plasmid-map-sequence-id-214262

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Showing: 4171 - 4200 of 4580 results