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pGEX2T E7 del EDE

Plasmid

#13635

Depositor

Karl Munger

Article

Wu et al J Virol. 1993 Apr . 67(4):2402-7.

Insert

HPV 16 E7 del EDE (E7 Human)

Expression

Bacterial

Mutation

del aa 35-37

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pGEX2T E7 L67R

Plasmid

#13649

Depositor

Karl Munger

Article

Wu et al J Virol. 1993 Apr . 67(4):2402-7.

Insert

HPV 16 E7 L67R (E7 Human)

Tags

GST

Expression

Bacterial

Mutation

aa 67 from L to R

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pGEX2T E7 L67K

Plasmid

#13650

Depositor

Karl Munger

Article

Wu et al J Virol. 1993 Apr . 67(4):2402-7.

Insert

HPV 16 E7 L67K (E7 Human)

Tags

GST

Expression

Bacterial

Mutation

aa 67 from L to K

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pGEX2T E7 L67E

Plasmid

#13651

Depositor

Karl Munger

Article

Wu et al J Virol. 1993 Apr . 67(4):2402-7.

Insert

HPV 16 E7 L67E (E7 Human)

Tags

GST

Expression

Bacterial

Mutation

aa 67 from L to E

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pGEX2T E7 del L67

Plasmid

#13652

Depositor

Karl Munger

Article

Wu et al J Virol. 1993 Apr . 67(4):2402-7.

Insert

HPV 16 E7 del L67 (E7 Human)

Tags

GST

Expression

Bacterial

Mutation

del aa 67

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pGEX2T E7 H2P

Plasmid

#13654

Depositor

Karl Munger

Article

Wu et al J Virol. 1993 Apr . 67(4):2402-7.

Insert

HPV 16 E7 H2P (E7 Human)

Tags

GST

Expression

Bacterial

Mutation

aa 2 from H to P

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
7861 pRS305-pUG72-HSC82out

Plasmid

#10899

Depositor

Susan Lindquist

Article

Cowen et al Science. 2005 Sep 30. 309(5744):2185-9.

Insert

HSC82 URA3 (HSC82 Budding Yeast)

Use

Cre/Lox

Expression

Yeast

Available Since

April 26, 2006

Availability

Academic Institutions and Nonprofits only

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pMT Ptp69D-GFP

Plasmid

#126663

Purpose

Inducible expression of Ptp69D-GFP in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Ptp69D

Expression

Insect

Mutation

A53S, K105N, and A98T- please see depositor comme…

Availability

Academic Institutions and Nonprofits only

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pET His10-Draper INT

Plasmid

#126670

Purpose

Expression of His10-Draper intracellular domain for purification from E. coli

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Draper cytoplasmic domain

Expression

Bacterial

Availability

Academic Institutions and Nonprofits only

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Human ICAM-3

Plasmid

#8857

Depositor

Timothy Springer

Article

de Fougerolles et al J Exp Med. 1992 Jan 1. 175(1):185-90.

Insert

ICAM-3 (ICAM3 Human)

Expression

Mammalian

Available Since

Oct. 4, 2005

Availability

Academic Institutions and Nonprofits only
pMXs-3XHA-EGFP-OMP25

Plasmid

#83356

Purpose

For tagging mitochondria with HA epitopes (HA-MITO construct)

Depositor

David Sabatini

Article

Chen et al Cell. 2016 Aug 25;166(5):1324-1337.e11.

Insert

3XHA-EGFP-OMP25

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-FLAG

Plasmid

#46956

Purpose

Plasmid for cloning and expression of GFP-FLAG tagged proteins (N-terminal tag). Confers resistance to G418.

Depositor

Steve Jackson

Article

Britton et al J Cell Biol. 2013 Jul 29.

Insert

FLAG tag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

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TRE-mCherry-p2A-eNpHR3.0-TS

Plasmid

#158703

Purpose

Reporter construct

Depositor

Alice Ting

Article

Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

Insert

mCherry-p2A-eNpHR3.0-TS

Use

AAV

Tags

mCherry

Expression

Mammalian

Promoter

TRE

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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HA-RNF126

Plasmid

#138647

Purpose

Mammalian expression of 2HA-tagged RNF126

Depositor

Jane McGlade

Article

Smith et al Exp Cell Res. 2014 Jan 15;320(2):219-32.

Insert

RNF126 (RNF126 Mouse)

Tags

HA-HA

Expression

Mammalian

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pET 15b-UbcH5a/2417

Plasmid

#11472

Depositor

Allan Weissman

Article

Jensen et al J Biol Chem. 1995 Dec 22. 270(51):30408-

Insert

UbcH5a (UBE2D1 Human)

Expression

Bacterial

Mutation

n/a

Available Since

April 5, 2006

Availability

Academic Institutions and Nonprofits only
pLGB13

Plasmid

#126618

Purpose

Suicide vector for allelic replacement in Bacteroides, erythromycin selection and aTC-inducible ss-Bfe1 counterselection

Depositor

Laurie Comstock

Article

Garcia-Bayona et al mBio. 2019 Aug 13;10(4). pii: mBio.01762

Type

Empty backbone

Use

Allellic exchange in bacteria

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pLGB31

Plasmid

#126621

Purpose

Suicide vector for allelic replacement in Bacteroides, inulin selection and rhamnose-inducible ss-Bfe1 counterselection

Depositor

Laurie Comstock

Article

Garcia-Bayona et al mBio. 2019 Aug 13;10(4). pii: mBio.01762

Type

Empty backbone

Use

Allellic exchange in bacteria

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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TRE-mCherry-p2A-Chrimson-TS

Plasmid

#158702

Purpose

Reporter construct

Depositor

Alice Ting

Article

Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

Insert

TRE-mcherry-p2A-Chrimson-TS

Use

Synthetic Biology

Tags

mCherry

Expression

Mammalian

Promoter

TRE

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pMXs-3XMyc-EGFP-OMP25

Plasmid

#83355

Purpose

For tagging mitochondria with Myc epitopes (Control-MITO construct)

Depositor

David Sabatini

Article

Chen et al Cell. 2016 Aug 25;166(5):1324-1337.e11.

Insert

3XMyc-EGFP-OMP25

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 26, 2016

Availability

Academic Institutions and Nonprofits only

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CMV-Tol2 transposase

Plasmid

#158774

Purpose

Expression of Tol2 transposase

Depositor

Hugo Snippert

Article

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

Tol2 tranposase

Expression

Mammalian

Mutation

Tol2 codon usage frequency well suitable for muri…

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p-cyclinB1-mcherry (1958)

Plasmid

#26063

Depositor

Jonathon Pines

Article

Gavet et al Dev Cell. 2010 Apr 20. 18(4):533-43.

Insert

Cyclin B1 (CCNB1 Human)

Tags

mcherry

Expression

Mammalian

Available Since

Sept. 17, 2010

Availability

Academic Institutions and Nonprofits only

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pET28:GFP

Plasmid

#60733

Purpose

pET28 driving expression of GFP, biobrick part Bba_E0040

Depositor

Matthew Bennett

Article

Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pRL056 pDESTattB_exorh:EGFP

Plasmid

#213510

Purpose

attB-containing pDEST plasmid, for use in Multisite Gateway reaction

Depositor

Christian Mosimann

Article

Lalonde et al Sci Adv. 2024 Jun 7;10(23):eadn6603. doi

Type

Empty backbone

Use

Zebrafish expression

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pJKR-H-tetR

Plasmid

#62561

Purpose

Tetracycline biosensor. High copy plasmid that expresses GFP proportional to tetracycline concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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LGmCreER (self deleting)

Plasmid

#33340

Depositor

Scott Lowe

Article

Zhao et al Genes Dev. 2010 Jul 1;24(13):1389-402.

Insert

CreERT2

Use

Cre/Lox and Retroviral

Expression

Mammalian

Promoter

PGK

Available Since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-1

Plasmid

#221472

Purpose

Gateway cloning of NKX2-1 short isoform overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway donor vector

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pCK122 pDESTattB_exorh:mCherry

Plasmid

#213511

Purpose

attB-containing pDEST plasmid, for use in Multisite Gateway reaction

Depositor

Christian Mosimann

Article

Lalonde et al Sci Adv. 2024 Jun 7;10(23):eadn6603. doi

Type

Empty backbone

Use

Zebrafish expression

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pRK-flag-GFP11

Plasmid

#78590

Purpose

Expression flag-GFP 11 in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-GFP 11

Tags

flag

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only
pRL092 pattB_cryaa:Venus_MCS

Plasmid

#213513

Purpose

attB-containing vector backbone, for use in restriction enzyme- or PCR-based cloning

Depositor

Christian Mosimann

Article

Lalonde et al Sci Adv. 2024 Jun 7;10(23):eadn6603. doi

Type

Empty backbone

Use

Zebrafish expression

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-3XFLAG-EGFP-OMP25

Plasmid

#83354

Purpose

For tagging mitochondria with Sigma FLAG epitopes

Depositor

David Sabatini

Article

Chen et al Cell. 2016 Aug 25;166(5):1324-1337.e11.

Insert

3XFLAG-EGFP-OMP25

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 26, 2016

Availability

Academic Institutions and Nonprofits only

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