We narrowed to 6,039 results for: WUS;

1. Lenti-FAB(PX-LD)

   Plasmid

   #214422

   Purpose

   Detection of peroxisome-lipid droplet contact site

   Depositor

   Chi-Lun Chang

   Article

   Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

   Insert

   PMP34-FKBP-V5-RspAN_IRES_CFAST-20nm-Halo-6xHp NC (SLC25A17 Human)
   

   Use

   Lentiviral

   Tags

   CFAST-Halo and FKBP-V5-RspAN

   Expression

   Mutation

   Promoter

   CMV

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pK27Sumo_His-SUMO-nsp12 (SARS-CoV-2)

   Plasmid

   #169188

   Purpose

   To express His-SUMO-nsp12 (SARS-CoV-2) in E. coli

   Depositor

   John Diffley

   Article

   Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

   Insert

   14His-SUMO-nsp12 (ORF1ab Synthetic, SARS-CoV-2)
   

   Use

   Tags

   14His-SUMO (Ulp1-cleavable)

   Expression

   Bacterial

   Mutation

   Codon optimised for E. coli

   Promoter

   T5

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. Anti-amyloid-β 40/42 nanobody (R3VQ) with sortase tag

   Plasmid

   #233217

   Purpose

   Mammalian epression of anti-amyloid-β 40/42 nanobody (R3VQ) with a sortase tag for direct dye conjugation.

   Depositor

   Jeff Lichtman

   Article

   Han et al bioRxiv 2023.10.24.563674

   Insert

   Anti-amyloid-β 40/42 nanobody (R3VQ)
   

   Use

   Tags

   Sortase tag, His tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti6/V5-DEST-HMGB1

   Plasmid

   #31208

   Depositor

   Lynda Chin

   Article

   Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

   Insert

   HMGB1 (HMGB1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 29, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pSimpleII-U6-sgRNA-BsmBI-NLS-NmCas9-HA-NLS

   Plasmid

   #115694

   Purpose

   All-in-one plasmid. Contains expression cassette for NmeCas9 with N and C NLS and HA tag at the C, plus cassette (for cloning of spacer in BsmBI) for expressing sgRNA under the control of U6 promoter.

   Depositor

   Erik Sontheimer

   Article

   Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

   Inserts

   NmeCas9
   Nme-sgRNA
   

   Use

   CRISPR

   Tags

   NLS and NLS, HA

   Expression

   Insect and Mammalian

   Mutation

   None and fusion of repeat/tracrRNA to make a sgRNA

   Promoter

   EF-1 alpha and U6

   Available since

   Sept. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT3-EF1aH-TAZ S89A

   Plasmid

   #179910

   Purpose

   Construct for expressing an activated form of TAZ (encoding be gene WWTR1)

   Depositor

   Xin Chen

   Article

   Wang et al J Hepatol. 2021 Aug 28. pii: S0168-8278(

   Insert

   WW Domain Containing Transcription Regulator 1 (WWTR1 Human)
   

   Use

   Tags

   3X Flag-tag

   Expression

   Mammalian

   Mutation

   Serine 89 mutated to Alanine

   Promoter

   EF1a

   Available since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. AXBE

   Plasmid

   #204605

   Purpose

   Mammalian expression of adenine transversion base editor AXBE

   Depositor

   Dali Li

   Article

   Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103

   Insert

   bpNLS-TadA-8e-nSpCas9-mAAG-bpNLS-P2A-GFP
   

   Use

   CRISPR

   Tags

   6*His

   Expression

   Mammalian

   Mutation

   S. pyogenes Cas9 (D10A)

   Promoter

   Available since

   Aug. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pK27Sumo_His-SUMO-nsp8 (SARS-CoV-2)

   Plasmid

   #169187

   Purpose

   To express His-SUMO-nsp8 (SARS-CoV-2) in E. coli

   Depositor

   John Diffley

   Article

   Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

   Insert

   14His-SUMO-nsp8 (ORF1ab Synthetic, SARS-CoV-2)
   

   Use

   Tags

   14His-SUMO (Ulp1-cleavable)

   Expression

   Bacterial

   Mutation

   Codon optimised for E. coli

   Promoter

   T5

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX_hDDX5_FL_wt

   Plasmid

   #88871

   Purpose

   inducible expression of GST-DDX5 in bacteria

   Depositor

   Dan Littman

   Article

   Huang et al Nature. 2015 Dec 24;528(7583):517-22. do

   Insert

   DDX5 (DDX5 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Tac

   Available since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-dCas12a-ABE8e

    Plasmid

    #193645

    Purpose

    Expression of base editor dCas12a-ABE8e in mammalian cells for base editing, along with non-fused EGFP fluorescence

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    dLbCas12a-TadA*8e, EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. FUW-[EF1α-CD19 4-1BB]

    Plasmid

    #218336

    Purpose

    Lentiviral transfer plasmids for mammalian expression CD19 CAR

    Depositor

    Haoyi Wang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    CD19 CAR mammalian expression cassette
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. FUW-[EF1α-CD19 CD28Z]

    Plasmid

    #218338

    Purpose

    Lentiviral transfer plasmids for mammalian expression CD19 CAR

    Depositor

    Haoyi Wang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    CD19 CAR mammalian expression cassette
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pICOZ-SB100X[EF1α-CD19 4-1BB]

    Plasmid

    #218328

    Purpose

    Donor plasmid of SB100X Transposon with CD19 CAR mammalian expression cassette

    Depositor

    Haoyi Wang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    CD19 CAR mammalian expression cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA5-FRT/TO-3*Flag-APEX2 N-term SRSF1

    Plasmid

    #187575

    Purpose

    Express FLAG epitope and APEX2-tagged SRSF1 fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    SRSF1 (SRSF1 Human)
    

    Use

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-Flag-PGC-1alpha1

    Plasmid

    #45501

    Depositor

    Jorge Ruas, Bruce Spiegelman

    Article

    Ruas et al Cell. 2012 Dec 7;151(6):1319-31. doi: 10

    Insert

    PGC-1alpha Isoform1 (Ppargc1a Mouse)
    

    Use

    Tags

    6xHis and Flag

    Expression

    Mammalian

    Mutation

    Q339K relative to NM_008904

    Promoter

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. DRH323: pAAV_hSyn-QuasAr2

    Plasmid

    #107705

    Purpose

    in vivo voltage imaging

    Depositor

    Adam Cohen

    Article

    Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

    Insert

    QuasAr2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pIG-730pUC19-tNGFR-P2A-Coronin1A

    Plasmid

    #186110

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    CORONIN1A
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Lenti-117G-hyA3A-BE4max

    Plasmid

    #157945

    Purpose

    Lentiviral expression of hyA3A-BE4max

    Depositor

    Dali Li

    Article

    Zhang et al Nat Cell Biol. 2020 Jun;22(6):740-750. d

    Insert

    Lenti-117G- hyA3A-BE4max
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti6/V5-DEST-MCM7

    Plasmid

    #31212

    Depositor

    Lynda Chin

    Article

    Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

    Insert

    MCM7 (MCM7 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

20. YA1529: pAAV_hSyn-QuasAr3-P2A-CheRiff

    Plasmid

    #107700

    Purpose

    in vivo voltage imaging

    Depositor

    Adam Cohen

    Article

    Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

    Insert

    QuasAr3-P2A-CheRiff
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin I

    Available since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. W118-1_hZBTB7B

    Plasmid

    #180380

    Purpose

    lentiviral transduction of human ZBTB7B gene

    Depositor

    Huda Zoghbi

    Article

    Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

    Insert

    ZBTB7B (ZBTB7B Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDYT002

    Plasmid

    #186550

    Purpose

    Lineage tracing vector (with barcoded Target Sites and triple sgRNAs

    Depositor

    Jonathan Weissman

    Article

    Yang et al Cell. 2022 Apr 28. pii: S0092-8674(22)00

    Insert

    14-bp random integration barcode and three target sites
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Aug. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLenti6/V5-DEST-RNF2

    Plasmid

    #31216

    Depositor

    Lynda Chin

    Article

    Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

    Insert

    RNF2 (RNF2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2012

    Availability

    Academic Institutions and Nonprofits only

24. pCOLADuet-1_nsp10 (SARS-CoV-2)

    Plasmid

    #169158

    Purpose

    To express SARS-CoV-2 nsp10 in E. coli

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

    Insert

    nsp10 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Methionine

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T7

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. K84M kinase-dead Nuak1-3xflag

    Plasmid

    #97219

    Purpose

    Plasmid expressing kinase-dead mutant Nuak1

    Depositor

    Huda Zoghbi

    Article

    Lasagna-Reeves et al Neuron. 2016 Oct 19;92(2):407-418. doi:

    Insert

    Nuak1 (NUAK1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Mutation

    Kinase-dead K84M

    Promoter

    T7

    Available since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA5-FRT/TO-3*Flag-APEX2 N-term NONO

    Plasmid

    #187580

    Purpose

    Express FLAG epitope and APEX2-tagged NONO fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    NONO (NONO Human)
    

    Use

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pUC19_T2A-H2B-mCitrine_loxP-hPGK-BSD-loxP

    Plasmid

    #195503

    Purpose

    SOX17 donor plasmid to generate locus specific C-terminal integration of H2B-mCitrine into the human SOX17-locus

    Depositor

    Alexander Meissner

    Article

    Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

    Insert

    SOX17(Ex2)-T2A-H2B-mCitrine_loxP-hPGK-BSD-loxP_SOX17(3'UTR) (SOX17 Human)
    

    Use

    CRISPR; Donor vector

    Tags

    T2A-H2B-mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pQLL-MAG[EF1α-copGFP]

    Plasmid

    #218324

    Purpose

    Donor plasmid of MAG Transposon with copGFP mammalian expression cassette

    Depositor

    Haoyi Wang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    copGFP mammalian expression cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pQLL-Tc1-1_Xt[EF1α-copGFP]

    Plasmid

    #218325

    Purpose

    Donor plasmid of Tc1-1_Xt Transposon with copGFP mammalian expression cassette

    Depositor

    Haoyi Wang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    copGFP mammalian expression cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCMV-Tc1-1_Xt

    Plasmid

    #217136

    Purpose

    Mammalian expression vector for expression of Tc1-1_Xt transposase (CMV promoter)

    Depositor

    Haoyi Wang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    Tc1-1_Xt transposase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge