Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 32,890 results for: cmv

Clear keywords
Showing: 5561 - 5600 of 32890 results

  1. pJEP11-AAV-U6/TO-sgRNA(Tet2)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid
    #82705
    Purpose
    AAV backbone with a full length U6/TO promoter driving expression of a sgRNA that targets exon 3 of the mouse Tet2 locus. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.
    Depositor
    Jonathan Ploski
    Article
    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do
    Insert
    U6/TO gRNA Expression Cassette Containing Tet2 gRNA
    Use
    AAV and CRISPR
    Tags
    CMV promoter driving expression of TetR-P2A-GFP-K…
    Expression
    Mammalian
    Available Since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    82705-plasmid-map-sequence-id-170450

    Enlarge

  2. pCMV-Frankenbody-HA-StayGold-IRESv4-HaloTag-tdMCP-tdP2A-tdPCP-mCherry

    Plasmid
    #241142
    Purpose
    Expresses Anti-HA frankenbody-StayGold, HaloTag-tdMCP, and tdPCP-mCherry to track mature and nascent HA-tagged proteins and track MS2 and PP7-tagged mRNA
    Depositor
    Tim Stasevich
    Article
    Galindo et al Mol Biol Cell. 2025 Jun 1;36(6):mr6. doi
    Inserts
    Anti-HA frankenbody
    tdMCP
    tdPCP
    Tags
    (n2)oxStayGold(c4) E147D, 3x mCherry, and HaloTag…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    241142-plasmid-map-sequence-id-487015

    Enlarge

  4. pMini-CMV-NLS-dead R-IscB-NLS_T2A_EGFP (Y67X)_LNMA intron

    Plasmid
    #246435
    Purpose
    Expresses inactive EGFP (Y67X) in mammalian cells for trans-splicing assessments. LMNA intron is inserted into EGFP CDS.
    Depositor
    Ailong Ke
    Article
    Xu et al Cell. 2025 Aug 18:S0092-8674(25)00854-2.
    Inserts
    O.gue IscB with dead mutations (D60A, H269A) and delta-TID
    EGFP
    Tags
    SV40 NLS, Thosea asigna virus 2A peptide, and nuc…
    Expression
    Mammalian
    Mutation
    changed Aspartic Acid 60 to Alanine, changed Hist…
    Promoter
    CMV
    Available Since
    Oct. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    246435-plasmid-map-sequence-id-488935

    Enlarge

  11. pCMV-T7-BPNLS-ABE8e(V106W)-SpRY-BPNLS-P2A-EGFP (CA136)

    Plasmid
    #208291
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e(V106W) A-to-G base editor with SpRY(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-ABE8e(V106W)-SpRY-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations and V106W in TadA and nSpRY(D10A/…
    Promoter
    CMV and T7
    Available Since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    208291-plasmid-map-sequence-id-410846

    Enlarge

  21. pTS113 pHAGE2 CMVtetO2 MCS-3C-SUMOEu-22xGS-Alfa-P2A-TagBFP

    Plasmid
    #199366
    Purpose
    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOEu-ALFA-P2A-BFP-tagged protein in mammalian cells.
    Depositor
    Rebecca Voorhees
    Article
    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    3C-SUMOEu-ALFA-P2A-TagBFP
    Expression
    Mammalian
    Mutation
    WT
    Available Since
    April 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    199366-plasmid-map-sequence-id-389910

    Enlarge

  22. pJEP12-AAV-H1/TO-L-sgRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid
    #82704
    Purpose
    AAV backbone with a full length H1/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl sites. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.
    Depositor
    Jonathan Ploski
    Article
    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do
    Insert
    H1/TO-L Empty gRNA Expression Cassette
    Use
    AAV and CRISPR
    Tags
    CMV promoter driving expression of TetR-P2A-GFP-K…
    Expression
    Mammalian
    Available Since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82704-plasmid-map-sequence-id-158051

    Enlarge

  23. pCMV-T7-BPNLS-CE-ABE8e-SpCas9(D10A)-BPNLS-P2A-EGFP (NK350)

    Plasmid
    #208292
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized CE-ABE8e-SpCas9 A-to-G base editor and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-CE-ABE8e-SpCas9-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA inlaid into nSpCas9(D10A)
    Promoter
    CMV and T7
    Available Since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    208292-plasmid-map-sequence-id-410847

    Enlarge

  28. pJEP13-AAV-H1/TO-L-sgRNA(Tet2)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid
    #82703
    Purpose
    AAV backbone with a full length H1/TO promoter driving expression of a sgRNA that targets exon 3 of the mouse Tet2 locus. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.
    Depositor
    Jonathan Ploski
    Article
    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do
    Insert
    H1/TO-L gRNA Expression Cassette Containing Tet2 gRNA
    Use
    AAV and CRISPR
    Tags
    CMV promoter driving expression of TetR-P2A-GFP-K…
    Expression
    Mammalian
    Available Since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82703-plasmid-map-sequence-id-158053

    Enlarge

  30. pHAGE2-TetOminiCMV-Oct4-p2a-Sox2AV-t2a-Klf4-e2a-Myc

    Plasmid
    #193347
    Purpose
    tetO-Sox2AV-KM (Dox-inducible reprogramming lentiviral vector expressing mouse Oct4 + Sox2A61V + Klf4 + cMyc)
    Depositor
    Hans Schöler
    Article
    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147
    Insert
    Oct4-p2a-Sox2AV-t2a-Klf4-e2a-cMyc (Klf4, Sox2, Pou5f1 Mouse)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    Sox2A61V is a highly cooperative point mutant of …
    Promoter
    TetO mini CMV
    Available Since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    193347-plasmid-map-sequence-id-379820

    Enlarge

  33. pJEP14-AAV-H1/TO-sgRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid
    #82702
    Purpose
    AAV backbone with a minimal H1/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.
    Depositor
    Jonathan Ploski
    Article
    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do
    Insert
    H1/TO Empty gRNA Expression Cassette
    Use
    AAV and CRISPR
    Tags
    CMV promoter driving expression of TetR-P2A-GFP-K…
    Expression
    Mammalian
    Available Since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82702-plasmid-map-sequence-id-163793

    Enlarge

  35. pTS102 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-SUMOstar-MCS

    Plasmid
    #199355
    Purpose
    Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-SUMOstar-tagged protein in mammalian cells.
    Depositor
    Rebecca Voorhees
    Article
    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    TagBFP-P2A-ALFA-SUMOstar
    Expression
    Mammalian
    Mutation
    WT
    Available Since
    April 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    199355-plasmid-map-sequence-id-389902

    Enlarge

  37. pJEP15-AAV-H1/TO-sgRNA(Tet2)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid
    #82701
    Purpose
    AAV backbone with a minimal H1/TO promoter driving expression of a sgRNA that targets exon 3 of the mouse Tet2 locus. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.
    Depositor
    Jonathan Ploski
    Article
    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do
    Insert
    H1/TO gRNA Expression Cassette Containing Tet2 gRNA
    Use
    AAV and CRISPR
    Tags
    CMV promoter driving expression of TetR-P2A-GFP-K…
    Expression
    Mammalian
    Available Since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82701-plasmid-map-sequence-id-157303

    Enlarge

  38. pCMV-T7-nSpRY(D10A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES636)

    Plasmid
    #185491
    Purpose
    CMV and T7 promoter expression plasmid for human codon usage nSpRY(D10A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP
    Depositor
    Benjamin Kleinstiver, Raul Perez-Jimenez
    Article
    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:
    Insert
    human codon usage nSpRY(D10A)-BPNLS-P2A-eGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-eGFP
    Expression
    Mammalian
    Mutation
    D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…
    Promoter
    CMV and T7
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185491-plasmid-map-sequence-id-362675

    Enlarge

  39. pTS115 pHAGE2 CMVtetO2 MCS-3C-SUMOstar-22xGS-Alfa-P2A-TagBFP

    Plasmid
    #199368
    Purpose
    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOstar-ALFA-P2A-BFP-tagged protein in mammalian cells.
    Depositor
    Rebecca Voorhees
    Article
    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    3C-SUMOstar-ALFA-P2A-TagBFP
    Expression
    Mammalian
    Mutation
    WT
    Available Since
    April 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    199368-plasmid-map-sequence-id-389900

    Enlarge

Showing: 5561 - 5600 of 32890 results