We narrowed to 4,949 results for: mcs

1. pEGFP N1 mitoAURKA

   Plasmid

   #157754

   Purpose

   Expression of AURKA:GFP targeted to mitochondria

   Depositor

   Marc Tramier

   Article

   Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

   Insert

   AURKA (AURKA Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   AURKA without the first 30 amino acids replaced b…

   Promoter

   CMV

   Available Since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. lenti-Cas9-VQR-GFP_activity_reporter

   Plasmid

   #87156

   Purpose

   This lentiviral vector can be used to assay activity of SpCas9-VQR (NGA PAM restricted).

   Depositor

   Daniel Bauer

   Article

   Canver et al Nat Genet. 2017 Feb 20. doi: 10.1038/ng.

   Insert

   GFP and sgRNA targeting GFP (NGA restricted)
   

   Use

   Lentiviral

   Available Since

   Feb. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223-GOLPH3

   Plasmid

   #21688

   Depositor

   Lynda Chin

   Article

   Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

   Insert

   Golgi Phosphoprotein 3 (GOLPH3 Human)
   

   Use

   Gateway entry clone

   Mutation

   last nucleotide of stop codon removed for c-termi…

   Available Since

   July 28, 2009

   Availability

   Academic Institutions and Nonprofits only

4. pMCB320-sgNC.ACOCA

   Plasmid

   #169836

   Purpose

   Expresses a negative control sgRNA

   Depositor

   Laura Attardi

   Article

   Bieging-Rolett et al Mol Cell. 2020 Nov 5;80(3):452-469.e9. d

   Insert

   safe harbor sgRNA
   

   Use

   CRISPR, Lentiviral, and Mouse Targeting

   Expression

   Mammalian

   Promoter

   mU6

   Available Since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lenti-sgZmat3.55.9/Cre

   Plasmid

   #167853

   Purpose

   Expresses Cre recombinase and an sgRNA targeting Zmat3

   Depositor

   Laura Attardi

   Article

   Bieging-Rolett et al Mol Cell. 2020 Nov 5;80(3):452-469.e9. d

   Insert

   sgRNA targeting Zmat3
   

   Use

   CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting

   Expression

   Mammalian

   Available Since

   May 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMCB320-sgZmat3.51.5

   Plasmid

   #169024

   Purpose

   Expresses an sgRNA targeting Zmat3

   Depositor

   Laura Attardi

   Article

   Bieging-Rolett et al Mol Cell. 2020 Nov 5;80(3):452-469.e9. d

   Insert

   sgRNA targeting Zmat3, mCherry, Puromycin resistance
   

   Use

   CRISPR, Lentiviral, and Mouse Targeting

   Expression

   Mammalian

   Promoter

   mU6

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMCB320-sgZmat3.55.9

   Plasmid

   #169026

   Purpose

   Expresses an sgRNA targeting Zmat3

   Depositor

   Laura Attardi

   Article

   Bieging-Rolett et al Mol Cell. 2020 Nov 5;80(3):452-469.e9. d

   Insert

   sgRNA targeting Zmat3, mCherry, Puromycin resistance
   

   Use

   CRISPR, Lentiviral, and Mouse Targeting

   Expression

   Mammalian

   Promoter

   mU6

   Available Since

   May 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMIR-31-Reporter

   Plasmid

   #71871

   Purpose

   Mammalian expression vector for the analysis of miR-31 activity

   Depositor

   Mien-Chie Hung

   Article

   Shen et al Nature. 2013 May 16;497(7449):383-7. doi

   Insert

   Reverse complementary sequence of miR-31
   

   Tags

   firefly luciferase

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. MDH1-miR-196a-1-PGK-GFP-Blast

   Plasmid

   #31509

   Depositor

   Chang-Zheng Chen

   Article

   Arnold et al Genome Res. 2011 May . 21(5):798-810.

   Insert

   miR-196a1 (Mir196a-1 Mouse)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pTwist-FLAG-SRSF3_Truncated_Codon.Optimized

    Plasmid

    #172346

    Purpose

    Expression of FLAG-tagged, codon-optimized, truncated SRSF3

    Depositor

    Sujatha Jagannathan

    Article

    Campbell et al Cell Rep. 2023 Jun 13;42(6):112642. doi:

    Insert

    serine and arginine rich splicing factor 3 (SRSF3 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    codon-optimized, truncated at amino acid 114 with…

    Promoter

    EF-1a

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV superYFP-AURKA K162M-mTurq2

    Plasmid

    #157773

    Purpose

    Expression of AuroraA kinase-dead biosensor under CMV promoter

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Tags

    mTurquoise2 and superYFP

    Expression

    Mammalian

    Mutation

    AURKA K162M is a kinase-dead version of AURKA

    Promoter

    CMV

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. t1-400

    Plasmid

    #166577

    Purpose

    For expression of human talin head (residues 1-400) in E. coli. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.

    Depositor

    Vesa Hytönen

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2020 Dec 22;11

    Insert

    Tln1 Head (1-400) (TLN1 Human)
    

    Tags

    His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…

    Expression

    Bacterial

    Mutation

    residues 1-400 only

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. LADL Bridge + Promoter Only Target

    Plasmid

    #127669

    Purpose

    Encodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter as well as 2 gRNAs targeting the Zfp462 promoter expressed from their individual hU6 promoters

    Depositor

    Jennifer Phillips-Cremins

    Article

    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

    Insert

    CRY2-HA-2A-mCherry and gRNAs 115 and 117
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Promoter

    EF1a and hU6

    Available Since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. Luc-Hdm4-3'UTR-F2d5

    Plasmid

    #64018

    Purpose

    Luciferase reporter of human Hdm4 3'UTR F2 fragment mutant #5

    Depositor

    Lin He

    Article

    Okada et al Genes Dev. 2014 Mar 1;28(5):438-50. doi:

    Insert

    HDMX (MDM4 Human)
    

    Expression

    Mammalian

    Available Since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. MDH1-miR-221SM-PGK-GFP-Blast

    Plasmid

    #31520

    Depositor

    Chang-Zheng Chen

    Article

    Arnold et al Genome Res. 2011 May . 21(5):798-810.

    Insert

    miR-221 (Mir221 Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    seed mutant control

    Available Since

    Sept. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

16. MDH1-miR-196a-1SM-PGK-GFP-Blast

    Plasmid

    #31510

    Depositor

    Chang-Zheng Chen

    Article

    Arnold et al Genome Res. 2011 May . 21(5):798-810.

    Insert

    miR-196a1 (Mir196a-1 Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    seed mutant control

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

17. Hoxa4-3'UTR

    Plasmid

    #31524

    Depositor

    Chang-Zheng Chen

    Article

    Arnold et al Genome Res. 2011 May . 21(5):798-810.

    Insert

    Hoxa4-3'UTR (Hoxa4 Mouse)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Available Since

    Oct. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

18. MDH1-miR-324SM-PGK-GFP-Blast

    Plasmid

    #31518

    Depositor

    Chang-Zheng Chen

    Article

    Arnold et al Genome Res. 2011 May . 21(5):798-810.

    Insert

    miR-324 (Mir324 Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    seed mutant control

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pREC1010-opEas1

    Plasmid

    #240701

    Purpose

    RSF1010 origin of replication plasmid containing Eas1 recombitron with extended a1 a2 regions in the ncRNA targeting lacZ locus in Klebsiella pneumoniae ATCC 10031 expressed by Pm promoter

    Depositor

    Seth Shipman

    Article

    González-Delgado et al bioRxiv 2025.06.16.660010

    Insert

    Eas1 RT, Eas1 ncRNA, CspRecT and EcSSB
    

    Expression

    Bacterial

    Mutation

    Extended a1 a2 regions

    Available Since

    Oct. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. TAPBPR S104F K211L R270Q

    Plasmid

    #245834

    Purpose

    expresses TAPBPR-S104F-K211L-R270Q in S2 cells

    Depositor

    Nikolaos Sgourakis

    Article

    Sun et al Sci Adv. 2023 Feb 24;9(8):eade7151. doi:

    Insert

    TAPBPR (S104F K211L R270Q) (TAPBPL Human)
    

    Tags

    AviTag biotinylation tag and His6 affinity tag

    Expression

    Insect

    Mutation

    S104F K211L R270Q (HiFi mutants) & C94S (remo…

    Promoter

    MT_puro

    Available Since

    Oct. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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