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WDR24 in pRK5

Plasmid

#154213

Purpose

Untagged WDR24 cDNA expression vector for transfections

Depositor

David Sabatini

Article

Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

Insert

WDR24 (WDR24 Human)

Tags

NONE

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pSF1C-A-RFP

Plasmid

#172431

Purpose

StrepFlex MoClo vector for cloning in E. coli and Streptomyces expression - equivalent of pTU1-A-RFP from EcoFlex (ACS Synth. Biol. 2016, 5, 10, 1059–1069)

Depositor

Paul Freemont, Simon Moore

Article

Moore et al ACS Synth Biol. 2021 Feb 19;10(2):402-41

Type

Empty backbone

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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TRE-UBC-mCD59-T2A-GFP

Plasmid

#205451

Purpose

lentiviral plasmid for expression of mouse CD59

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

mCD59 (Cd59a Mouse)

Expression

Mammalian

Promoter

UBC

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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p3XFlag-Mettl4

Plasmid

#86665

Purpose

Expresses murine Mettl4 containing N-terminal 3XFlag tag

Depositor

Douglas Feldman

Article

Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

Insert

Mettl4 (Mettl4 Mouse)

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only

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GB1-His-TET1-848-952(pL91)

Plasmid

#124388

Purpose

E.Coli produced TET1-848-952 purified by FPLC nickel column

Depositor

Shaun Cowley

Article

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

GB1-His-TET1-848-952 wildtype (TET1 Human)

Tags

GB1-Histidine

Expression

Bacterial

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-TDP-43-D169G

Plasmid

#133757

Purpose

Lentiviral expression of human TDP-43 D169G disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed aspartate 169 to glycine.

Promoter

CMV

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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KTK_354

Plasmid

#180525

Purpose

KTK compatible Intergration/suicide plasmid. Contains a LacZ dropout cassette flanked by regions homolgous to the arsenic resistance cassete.

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Type

Empty backbone

Expression

Bacterial

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-CenpB 1-166

Plasmid

#108475

Purpose

expression of GFP-CenpB 1-166

Depositor

Susanne Lens

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

CenpB 1-166 AA (CENPB Human)

Tags

EGFP

Expression

Mammalian

Mutation

encodes only the DNA-binding domain of CENP-B

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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Bov.hsc70.RSET.FL.wt(NarI)

Plasmid

#12532

Depositor

David McKay

Article

Ha et al J Biol Chem. 1997 Oct 31. 272(44):27796-

Insert

bovine 70 kDa heat shock cognate protein full length wild type

Expression

Bacterial

Mutation

wild type

Available Since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only

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pMP71-tCD19-LLO-SIINFEKL

Plasmid

#174598

Purpose

expresses the extracellular and transmembrane domain of murine CD19 with a C terminal fusion of the LLO190 and SIINFEKL epitopes

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

membrane bound CD19, LLO antigen, SIINFEKL (ovalbumin) antigen

Expression

Mammalian

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR1A DHFR.cHSF1

Plasmid

#134737

Purpose

Gateway entry vector for constitutively active HSF1 fused to the DHFR destabilized domain

Depositor

Matthew D. Shoulders

Article

Phillips et al Elife. 2017 Sep 26;6. doi: 10.7554/eLife

Insert

DHFR.cHSF1 (HSF1 Human)

Use

Entry vector for gateway cloning

Tags

DHFR

Mutation

Deletion of amino acids 186-202

Promoter

None

Available Since

Oct. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5 CASTOR2-HA Cterm

Plasmid

#84489

Purpose

overexpression of CASTOR2 with HA tag

Depositor

David Sabatini

Article

Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

Insert

CASTOR2 (GATSL2 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only
PIGAZFN-L1

Plasmid

#37797

Depositor

Linzhao Cheng

Article

Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

Insert

PIGA zinc finger L1

Expression

Mammalian

Promoter

CMV

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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pCRISPR_DMS3g24

Plasmid

#89244

Purpose

Plasmid encodes a synthetic CRISPR containing 7 copies of a single spacer, which targets a region (gene 24) in the genome of bacteriophage DMS3

Depositor

Blake Wiedenheft

Article

Chowdhury et al Cell. 2017 Mar 23;169(1):47-57.e11. doi:

Insert

CRISPR

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pENN.AAV.CB7.CI.CNDP1-flag.WPRE.rBG

Plasmid

#132683

Purpose

AAV plasmid encoding mouse CNDP1 with C-terminal flag tag

Depositor

Jonathan Long

Article

Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

Insert

Carnosine dipeptidase 1 (Cndp1 Mouse)

Use

AAV

Tags

Flag

Promoter

Chicken b-actin

Available Since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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PIGAZFN-R1

Plasmid

#37799

Depositor

Linzhao Cheng

Article

Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

Insert

PIGA zinc finger R1

Expression

Mammalian

Promoter

CMV

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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pRGEB-VirD2-Cas9

Plasmid

#139803

Purpose

Expressing VirD-Cas9 fusion under ubiquitin promoter

Depositor

Magdy Mahfouz

Article

Ali et al Commun Biol. 2020 Jan 23;3(1):44. doi: 1

Insert

VirD2-Cas9

Tags

VirD2-Cas9

Expression

Plant

Promoter

Ubiquitin

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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p3xFlag-Alkbh4

Plasmid

#105240

Purpose

Expresses murine Alkbh4 containing an N-ternimal 3xFlag tag

Depositor

Douglas Feldman

Article

Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

Insert

Alkbh4 (Alkbh4 Mouse)

Tags

Flag and HA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pDL001_Hec1-ms_IgG_HC

Plasmid

#193618

Purpose

Hec1 heavy chain variable and constant region (mouse) + exogenous N-term signal peptide. For co-expression with pDL002 in HEK293 cells.

Depositor

Jennifer DeLuca

Article

DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

Insert

Anti-Hec1 Heavy Chain (Hec1-ms_IgG_HC)

Tags

His6 and signal peptide

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pDL002_Hec1-ms_IgG_LC

Plasmid

#193619

Purpose

Hec1 light chain variable and constant region (mouse) + exogenous N-term signal peptide. For co-expression with pDL001 in HEK293 cells.

Depositor

Jennifer DeLuca

Article

DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

Insert

Anti-Hec1 light chain (Hec1-ms_IgG_LC)

Tags

signal peptide

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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