4,177 results

1. pY108 (lenti-AsCpf1)

   Plasmid

   #84739

   Purpose

   Lenti virus delivery of AsCpf1 and crRNA guide

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

   Inserts

   huAsCpf1
   puromycin resistance gene
   

   Use

   Lentiviral

   Tags

   3xHA, NLS, and P2A

   Promoter

   EFS

   Available Since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PGK1p-Csy4-pA (Construct 2)

   Plasmid

   #55196

   Purpose

   Expresses Csy4 in Mammalian cells for site-specific RNA processing

   Depositor

   Timothy Lu

   Article

   Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

   Insert

   Csy4/Cas6
   

   Use

   Synthetic Biology

   Tags

   6xHIS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNLS-iRFP670

   Plasmid

   #45466

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

   Insert

   iRFP670
   

   Tags

   nuclear localization signal

   Expression

   Mammalian

   Mutation

   T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

   Promoter

   CMV

   Available Since

   June 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. HA-cdh1

   Plasmid

   #11596

   Depositor

   Marc Kirschner

   Article

   Pfleger et al Genes Dev. 2001 Sep 15. 15(18):2396-407.

   Insert

   cdh1 (FZR1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. CD20

   Plasmid

   #1890

   Depositor

   Sander van den Heuvel

   Article

   van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

   Insert

   CD20 (MS4A1 Human)
   

   Expression

   Mammalian

   Available Since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDH-puro-CMV-VC3AI

   Plasmid

   #78907

   Purpose

   Indicator of caspase-3-like protease activity

   Depositor

   Binghui Li

   Article

   Zhang et al Nat Commun. 2013;4:2157. doi: 10.1038/nc

   Insert

   VC3AI
   

   Use

   Lentiviral

   Tags

   HA and Myc

   Mutation

   S320A, deletion of AA1-200

   Promoter

   CMV

   Available Since

   June 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSUMO1

   Plasmid

   #52258

   Purpose

   Bacterial expression of human 6His-tagged SUMO1, SUMO-E1 and SUMO-E2

   Depositor

   Primo Schaer

   Article

   Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

   Inserts

   SUMO-1 (SUMO1 Human)
   UBE2l (UBE2I Human)
   SAE-2 (UBA2 )
   SAE-1 (SAE1 Human)
   

   Tags

   6His-tag

   Expression

   Bacterial

   Mutation

   C-terminal gly-gly

   Promoter

   T7 and rbs

   Available Since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pY109 (lenti-LbCpf1)

   Plasmid

   #84740

   Purpose

   Lenti virus delivery of LbCpf1 and crRNA guide

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

   Inserts

   huLbCpf1
   puromycin resistance gene
   

   Use

   Lentiviral

   Tags

   3xHA, NLS, and P2A

   Promoter

   EFS

   Available Since

   Nov. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pKIR1.1

   Plasmid

   #85758

   Purpose

   CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter

   Depositor

   Tetsuya Higashiyama

   Article

   Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

   Insert

   human-codon-optimized SpCas9
   

   Use

   CRISPR

   Tags

   FLAG

   Expression

   Plant

   Promoter

   AtRPS5A

   Available Since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmCherry-C1-mTrim21

    Plasmid

    #105516

    Purpose

    Expression of m-Cherry-tagged wildtype mouse Trim21 in mammalian cells

    Depositor

    Melina Schuh

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    TRIM21 (Trim21 Mouse)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBiFC-VN155(I152L)

    Plasmid

    #27097

    Depositor

    Chang-Deng Hu

    Article

    Kodama et al Biotechniques. 2010 Nov . 49(5):793-805.

    Insert

    VN155(I152L)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    I152L

    Available Since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. HA-NEDD8

    Plasmid

    #18711

    Depositor

    Edward Yeh

    Article

    Kamitani et al J Biol Chem. 1997 Nov 7. 272(45):28557-6

    Insert

    NEDD8 (NEDD8 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CaV1.2

    Plasmid

    #26572

    Depositor

    Diane Lipscombe

    Article

    Helton et al J Neurosci. 2005 Nov 2. 25(44):10247-51.

    Insert

    Calcium channel, voltage-dependent, L type, alpha 1C subunit (Cacna1c Mouse)
    

    Expression

    Mammalian

    Available Since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCI-EGFP-NR1 wt

    Plasmid

    #45446

    Depositor

    Andres Barria, Robert Malinow

    Article

    Barria et al Neuron. 2002 Jul 18;35(2):345-53.

    Insert

    NR1 (Grin1 Rat)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    EGFP inserted after the predicted signal peptide …

    Promoter

    CMV

    Available Since

    June 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV CMV-dSaCas9-KRAB-bGHpA

    Plasmid

    #106219

    Purpose

    AAV expressing deactivated S. aureus Cas9 fused to a KRAB repressor

    Depositor

    Charles Gersbach

    Article

    Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

    Insert

    dead S. aureus Cas9 KRAB
    

    Use

    AAV and CRISPR

    Tags

    HA-NLS

    Expression

    Mammalian

    Mutation

    D10A and N580A

    Promoter

    CMV

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLVCT-tTR-KRAB

    Plasmid

    #11643

    Purpose

    Tet-regulated (Tet-on) lentiviral vector for transgene (CAG promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

    Depositor

    Patrick Aebischer, Didier Trono

    Article

    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

    Insert

    CAG promoters, GFP, tTR-KRAB, Tet-on
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p4489 Flag-betaTrCP

    Plasmid

    #10865

    Depositor

    Peter Howley

    Article

    Zhou et al Mol Cell. 2000 Sep . 6(3):751-6.

    Insert

    betaTrCP (BTRC Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Aug. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mRFP-Clc

    Plasmid

    #14435

    Depositor

    Ari Helenius

    Article

    Tagawa et al J Cell Biol. 2005 Aug 29. 170(5):769-79.

    Insert

    Clathrin light chain (Clc Mouse)
    

    Tags

    mRFP

    Expression

    Mammalian

    Available Since

    March 23, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GFP-PTEN

    Plasmid

    #13039

    Depositor

    Alonzo Ross

    Article

    Liu et al J Cell Biochem. 2005 Oct 1. 96(2):221-34

    Insert

    PTEN (PTEN Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mTOR_1 shRNA

    Plasmid

    #1855

    Depositor

    David Sabatini

    Article

    Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

    Insert

    mTOR (MTOR Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    June 23, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge