4,235 results

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pLenti SpBsmBI sgRNA Hygro

Plasmid

#62205

Purpose

An empty sgRNA expression vector for expression of sgRNA for Sp Cas9

Depositor

Rene Maehr

Article

Pham et al Methods Mol Biol. 2016;1358:43-57. doi:

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

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ES-FUCCI

Plasmid

#62451

Purpose

SMX_DEST vector with 3 cassettes

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Inserts

minPGK::Citrine-Geminin(1-110)-bGHpA (PGKEnhancer)x5
minPGK::Cherry-hCdt1(30-120)-bGHpA
PGK::HygroR-bGpA

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pCM157

Plasmid

#45863

Depositor

Mary Lidstrom

Article

Marx et al Biotechniques. 2002 Nov;33(5):1062-7.

Insert

cre recombinase

Use

Cre/Lox

Expression

Bacterial

Promoter

lac

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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pCSDest

Plasmid

#22423

Depositor

Nathan Lawson

Article

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Type

Empty backbone

Available Since

Dec. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-sfCherry(1-10)

Plasmid

#70222

Purpose

Expresses sfCherry(1-10) in mammalian cells

Depositor

Bo Huang

Article

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Insert

sfCherry(1-10)

Expression

Mammalian

Available Since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pMA7CR_2.0

Plasmid

#73950

Purpose

pMA7CR_2.0 contains arabinose inducible λ/RED β-protein and dam, and aTc inducible CRISPR/Cas9 and recX

Depositor

Alex Nielsen

Article

Ronda et al Sci Rep. 2016 Jan 22;6:19452. doi: 10.10

Inserts

cas9
lambda beta
dam
recX

Expression

Bacterial

Promoter

pAra and pTet

Available Since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-Caspase1-flag

Plasmid

#21142

Depositor

Junying Yuan

Article

Li et al J Cell Biol. 2008 Apr 21. 181(2):321-33.

Insert

caspase-1 (Casp1 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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CTV

Plasmid

#15912

Depositor

Klaus Rajewsky

Article

Thai et al Science. 2007 Apr 27. 316(5824):604-8.

Insert

CAG-STOP-eGFP-ROSA26TV

Use

Cre/Lox; /stop

Available Since

Oct. 26, 2007

Availability

Academic Institutions and Nonprofits only

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a1Ha-pcDNA3

Plasmid

#45809

Depositor

Edward Perez-Reyes

Article

Cribbs et al Circ Res. 1998 Jul 13;83(1):103-9.

Insert

a1Ha (CACNA1H Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 13, 2013

Availability

Academic Institutions and Nonprofits only

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pGEM-Sox2

Plasmid

#16353

Depositor

James Thomson

Article

Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

Insert

Homo sapiens SRY (sex determining region Y)-box 2 (SOX2) (SOX2 Human)

Expression

Bacterial

Available Since

Jan. 4, 2008

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-SARS-CoV-2-nsp2-2xStrep-IRES-Puro

Plasmid

#141368

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp2 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 8, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pSH-EFIRES-B-Seipin-miniIAA7-mEGFP

Plasmid

#129719

Purpose

AAVS1 safe harbor targeting vector expressing miniIAA7-mEGFP tagged Seipin

Depositor

Elina Ikonen

Article

Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

Insert

Seipin (BSCL2 Human)

Use

CRISPR and TALEN; Human safe harbor locus (a avs1…

Tags

miniIAA7-mEGFP

Expression

Mammalian

Mutation

silent mutations

Promoter

EF1a

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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LacZ in pLX304

Plasmid

#42560

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

LacZ

Use

Lentiviral

Tags

2xV5

Promoter

CMV

Available Since

March 11, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Drp1K38A

Plasmid

#45161

Depositor

Alexander van der Bliek, Richard Youle

Article

Smirnova et al J Cell Biol. 1998 Oct 19;143(2):351-8.

Insert

Drp1 (DNM1L Human)

Tags

HA (AYPYDVPDY)

Expression

Mammalian

Mutation

K38A

Promoter

CMV

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP8

Plasmid

#22608

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP8 (USP8 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Dec. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pCMV5B-Flag-Smurf2 wt

Plasmid

#11746

Depositor

Jeff Wrana

Article

Kavsak et al Mol Cell. 2000 Dec . 6(6):1365-75.

Insert

SMURF2 (SMURF2 Human)

Tags

Flag

Expression

Mammalian

Available Since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pCX-OKS-2A

Plasmid

#19771

Purpose

Non-integrating (episomal) expression of mouse Oct3/4, Klf4 and Sox2

Depositor

Shinya Yamanaka

Article

Okita et al Science. 2008 Nov 7. 322(5903):949-53.

Insert

Oct3/4-2A-Klf4-2A-Sox2 (Sox2, Klf4, Pou5f1 Mouse)

Expression

Mammalian

Mutation

Three genes are connected with the foot-and-mouth…

Available Since

Jan. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pCAG-HIF1 alpha

Plasmid

#21101

Depositor

Connie Cepko

Article

Chen et al Science. 2009 Jan 9. 323(5911):256-9.

Insert

HYPOXIA INDUCIBLE FACTOR 1ALPHA (HIF1A Human)

Expression

Mammalian

Available Since

May 5, 2009

Availability

Academic Institutions and Nonprofits only

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phage-cmv-cfp-24xms2

Plasmid

#40651

Depositor

Robert Singer

Article

Wu et al Biophys J. 2012 Jun 20;102(12):2936-44.

Insert

CFP-24xMBS

Use

Lentiviral

Tags

CFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pET GFP LIC cloning vector (u-msfGFP)

Plasmid

#29772

Depositor

Scott Gradia

Type

Empty backbone

Tags

SiriusGFP

Expression

Bacterial

Promoter

T7

Available Since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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