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pLVUHshp53

Plasmid

#11653

Purpose

3rd generation lentiviral transfer vector expressing shp53 under an hUbiquitin promoter.

Depositor

Patrick Aebischer, Didier Trono

Article

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

GFP, shRNA against TP53 (TP53 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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CaV2.1/pcDNA3.1 ~ Human

Plasmid

#140575

Purpose

Expression in mammallian cells or oocytes

Depositor

Dan Minor

Article

Abderemane-Ali et al Neuron. 2019 Mar 20;101(6):1134-1149.e3.

Insert

CaV2.1 Human (CACNA1A Human)

Expression

Mammalian

Promoter

CMV and T7

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pPBbleo-ErbB1

Plasmid

#197359

Purpose

Encoding human ErbB1.

Depositor

Michiyuki Matsuda

Article

Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

Insert

EGFR (EGFR Human)

Expression

Mammalian

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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Vim-mCh-FKBP

Plasmid

#240423

Purpose

Tagged vimentin for rapalog induced pulling experiments.

Depositor

Anna Akhmanova

Article

Pasolli et al J Cell Biol. 2025 Sep 1;224(9):e20250400

Insert

Vimentin (VIM Human)

Tags

FKBP and mCherry

Expression

Mammalian

Available Since

Aug. 1, 2025

Availability

Academic Institutions and Nonprofits only

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Vim-mCh-SspB

Plasmid

#240421

Purpose

Tagged vimentin for optogenetic vimentin pulling experiments.

Depositor

Anna Akhmanova

Article

Pasolli et al J Cell Biol. 2025 Sep 1;224(9):e20250400

Insert

Vimentin (VIM Human)

Tags

SspB and mCherry

Expression

Mammalian

Available Since

July 30, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti6/V5-p53_R280K

Plasmid

#22933

Depositor

Bernard Futscher

Article

Junk et al Neoplasia. 2008 May . 10(5):450-61.

Insert

tumor protein 53 (TP53 Human)

Use

Lentiviral

Tags

v5

Expression

Mammalian

Mutation

R280K mutant

Available Since

Jan. 27, 2010

Availability

Academic Institutions and Nonprofits only
pGL410_INS421

Plasmid

#49057

Purpose

Expresses luciferase under control of human insulin gene promoter

Depositor

Kevin Ferreri

Article

Kuroda et al PLoS One. 2009 Sep 9;4(9):e6953. doi: 10

Insert

human INS promoter (INS Human)

Expression

Mammalian

Promoter

INS promoter

Available Since

Oct. 30, 2013

Availability

Academic Institutions and Nonprofits only

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p27-IRES-mCherry

Plasmid

#80143

Purpose

retroviral expression of p27 and mCherry

Depositor

Ellen Rothenberg

Article

Kueh et al Science. 2013 Aug 9;341(6146):670-3. doi

Insert

p27 (CDKN1B Human)

Use

Retroviral

Tags

IRES-mCherry

Expression

Mammalian

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pRSF-G1(5CNW)RS

Plasmid

#222872

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of 5-Cyano-L-Tryptophan (5-CN-Trp) into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-Actin-C-18

Plasmid

#54967

Purpose

Localization: Actin, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

Actin (ACTB Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1 3xHA-MEK1-DD

Plasmid

#47576

Purpose

Bacterial expression plasmid for GST-3xHA-tagged constitutively active MEK1

Depositor

Kevin Janes

Article

Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

Insert

MEK1-DD (Map2k1 Mouse)

Tags

3xHA and GST

Expression

Bacterial

Mutation

Ser218 to Asp, Ser221 to Asp

Available Since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only
pmScarlet_alphaTubulin_C1

Plasmid

#85045

Purpose

In vivo visualization of microtubules (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

Alpha-Tubulin (TUBA1B Human)

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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AICSDP-43: GJA1-mEGFP

Plasmid

#109120

Purpose

Homology arms and linker-mEGFP sequence for C-terminus tagging of human GJA1

Depositor

Allen Institute for Cell Science

Insert

GJA1 Homology Arms with linker-mEGFP (GJA1 Human)

Use

CRISPR; Donor template

Tags

linker-mEGFP

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Available Since

May 4, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pBGPa-CMV-GFP-OSBP PH domain

Plasmid

#58724

Purpose

Expresses GFP-Tagged OSBP PH domain in mammalian cells

Depositor

Tim Levine

Article

Levine et al Curr Biol. 2002 Apr 30;12(9):695-704.

Insert

Oxysterol-binding Protein PH domain (OSBP Human)

Tags

GFP and myc

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only
HIP1R-GFP-FKBP

Plasmid

#100752

Purpose

Expression of HIP1R with a GFP-FKBP tag in mammalian cells.

Depositor

Stephen Royle

Article

Clarke et al Nat Commun. 2018 Jul 4;9(1):2604. doi: 1

Insert

HIP1R (HIP1R Human)

Tags

FKBP and GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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Flag-ZDHHC22

Plasmid

#231670

Purpose

Expresses human ZDHHC22 protein

Depositor

Chunhong Ma

Article

Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

Insert

ZDHHC22 (ZDHHC22 Human)

Tags

3×Flag Tag

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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HER2 CA (V659E)

Plasmid

#16259

Depositor

Mien-Chie Hung

Article

Li et al Cancer Cell. 2004 Nov . 6(5):459-69.

Insert

HER2 (ERBB2 Human)

Expression

Mammalian

Mutation

Constitutively active: V659E

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

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hCD4-mOrange

Plasmid

#110192

Purpose

Encodes for human CD4 coding sequence tagged with the mOrange FP

Depositor

Sergi Padilla Parra

Article

Iliopoulou et al Nat Struct Mol Biol. 2018 Sep;25(9):814-

Insert

hCD4 (CD4 Human)

Tags

mOrange

Expression

Mammalian

Promoter

CMV

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pmTurquoise2-Tubulin

Plasmid

#36202

Purpose

In vivo visualization of microtubules (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

Alpha-tubulin (TUBA1B Human)

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-GABARAP WT

Plasmid

#123097

Purpose

Expresses 3xFLAG-GABARAP wild-type in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein (GABARAP Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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