We narrowed to 4,223 results for: 424

1. pX459-HypaCas9-mR2-ECT2_sgRNA

   Plasmid

   #183875

   Purpose

   pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ECT2 sgRNA for N-terminal tagging of Ect2 in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   ECT2 sgRNA spacer (ECT2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. EGFP-SopB(K525A)

   Plasmid

   #183659

   Purpose

   N-terminally tagged Salmonella Typhimurium SopB (K525A) for mammalian expression

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   K525A

   Promoter

   CMV

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. EGFP-SopB(K528A)

   Plasmid

   #183660

   Purpose

   N-terminally tagged Salmonella Typhimurium SopB (K528A) for mammalian expression

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   K528A

   Promoter

   CMV

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. EGFP-SopB(33-554)

   Plasmid

   #183661

   Purpose

   N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Amino acids 33-554

   Promoter

   CMV

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. EGFP-SopB(33-520)

   Plasmid

   #183662

   Purpose

   N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Amino acids 33-520

   Promoter

   CMV

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. EGFP-SopB(68-554)

   Plasmid

   #183663

   Purpose

   N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Amino acids 68-554

   Promoter

   CMV

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pESC-LEU-IpgD(C439S)

   Plasmid

   #183673

   Purpose

   Inducible Shigella flexneri IpgD (catalytically inactive) for expression in yeast

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   IpgD
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   C439S

   Promoter

   GAL1

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. SopB(C460S)-EGFP

   Plasmid

   #183656

   Purpose

   C-terminally tagged Salmonella Typhimurium SopB (catalytically inactive) for mammalian expression

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   C460S

   Promoter

   CMV

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. EGFP-SopB(C460S)

   Plasmid

   #183658

   Purpose

   N-terminally tagged Salmonella Typhimurium SopB (catalytically inactive) for mammalian expression

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   C460S

   Promoter

   CMV

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. FAM207A_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176950

    Purpose

    Mammalian expression vector encoding FAM207A and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    FAM207A (C21orf70 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pnEA-NpH-HsNot9_19-285-V181E_AE

    Plasmid

    #148617

    Purpose

    Bacterial Expression of HsNot9_19-285-V181E

    Depositor

    Elisa Izaurralde

    Article

    Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

    Insert

    HsNot9_19-285-V181E (CNOT9 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pT7-EGFP-C1-HsNot9-shRNAres2_W

    Plasmid

    #147905

    Purpose

    Mammalian Expression of HsNot9-shRNAres2

    Depositor

    Elisa Izaurralde

    Article

    Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

    Insert

    HsNot9-shRNAres2 (CNOT9 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pB80-KIF1A(1-365)-mVenus-SSPB(micro)

    Plasmid

    #174635

    Purpose

    Non-processive monomeric KIF1A for optogenetic heterodimerization to iLID via SSPB(micro)

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    KIF1A(1-365)-mVenus-SSPB(micro) (Kif1a Synthetic, Mouse)
    

    Use

    Tags

    mVenus-SSPB(micro)

    Expression

    Mammalian

    Mutation

    mmKIF1A(aa1-365): Pro202Ala; mVenus: Met1Del, Thr…

    Promoter

    Chicken beta-actin

    Available since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pB80-KIF1A(1-383)-GFP-SSPB(micro)

    Plasmid

    #174627

    Purpose

    Constitutively active KIF1A for optogenetic heterodimerization to iLID via SSPB(micro)

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    KIF1A(1-383)-GFP-SSPB(micro) (Kif1a Synthetic, Mouse)
    

    Use

    Tags

    GFP-SSPB(micro)

    Expression

    Mammalian

    Mutation

    mmKIF1A(aa1-383): Pro202Ala; EGFP: Met1Del; SSPB:…

    Promoter

    Chicken beta-actin

    Available since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAB(EXPR-HDAC5-trun1)

    Plasmid

    #114400

    Purpose

    For PYL-HDAC5 aa 1-275 expression

    Depositor

    Vincent Dion

    Article

    Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

    Insert

    PYL-HDAC5 aa 1-275 (HDAC5 Human)
    

    Use

    Tags

    3xFlag-NLS (internal)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pnCS_mEGFP-DSep2_Peanut-Strep

    Plasmid

    #174496

    Purpose

    bacterial co-expression of Drosophila Sep2 fused to monomeric EGFP and of Drosophila Peanut

    Depositor

    Manos Mavrakis

    Article

    Mavrakis et al Methods Cell Biol. 2016;136:199-220. doi

    Inserts

    Sep2 (Sep2 Fly)
    peanut (pnut Fly)
    

    Use

    Tags

    Strep and mEGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET-DEST42-dre4

    Plasmid

    #170440

    Purpose

    Express drosophila dre4 in E.coli expression system. Generated from pDONR-dre4 by Gateway system. Used for custom antibody purification.

    Depositor

    Joan Conaway

    Article

    Tettey et al Cell Rep. 2019 Jun 25;27(13):3770-3779.e

    Insert

    dre4 (dre4 Fly)
    

    Use

    Tags

    6xHis and V5

    Expression

    Bacterial

    Mutation

    Deleted amino acids 1-20

    Promoter

    T7

    Available since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEBTetDBl-CLIP-Cep63

    Plasmid

    #136861

    Purpose

    Mammalian expression of the centrosomal protein Cep63 N-terminally fused to CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    CLIP-Cep63 (CEP63 Synthetic, Human)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEBTetBl-CLIP-Cep63

    Plasmid

    #136860

    Purpose

    Mammalian expression of the centrosomal protein Cep63 N-terminally fused to CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    CLIP-Cep63 (CEP63 Synthetic, Human)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLEX-FHH-NRASQ61K/T35A-IRES-Puro

    Plasmid

    #120571

    Purpose

    Expresses Flag-HA-6xHIS-N-Ras Q61K/T35A fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    NRAS (NRAS Human)
    

    Use

    Lentiviral

    Tags

    Flag-HA-6xHIS

    Expression

    Mammalian

    Mutation

    Q61K, T35A

    Promoter

    CMV

    Available since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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