We narrowed to 17,732 results for: ERG

1. L2380-FKBP-GPA(V84R)-20-114

   Plasmid

   #222900

   Purpose

   Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 114

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FKBP-GPA(V84R)-20GS-NanoLuc114
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   V84R in GPA

   Promoter

   CMV

   Available Since

   Aug. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. L2378-FRB-GPA(V84R)-20-11S

   Plasmid

   #222899

   Purpose

   Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 11S.

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FRB-GPA(V84R)-20GS-NanoLuc11S
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   V84R in GPA

   Promoter

   CMV

   Available Since

   Aug. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. L2377-FRB-GPA(V84R)-20-114

   Plasmid

   #222898

   Purpose

   Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 114.

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FRB-GPA(V84R)-20GS-NanoLuc114
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   V84R in GPA

   Promoter

   CMV

   Available Since

   Aug. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. L2085-WT-GPA-NLuc-Biosensor-Retrovirus-TD135

   Plasmid

   #222875

   Purpose

   Retroviral vector encoding biosensor chains to detect rapamycin (FRB/FKBP domains) and respond with split NanoLuciferase reconstitution (11S/114 fragment). WT Glycophorin A (GPA) scaffold.

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FRB-GPA(WT)-NanoLuc114-T2A-FKBP-GPA(WT)-NanoLuc11S
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   MSCV LTR

   Available Since

   Aug. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGGI_Myr-YFP-MiniTurboID

   Plasmid

   #222432

   Purpose

   Potential plasma membrane control.

   Depositor

   Dominique Bergmann

   Article

   Wallner et al Dev Cell. 2024 May 6;59(9):1096-1109.e5.

   Insert

   MiniTurboID (BirA mutant)
   

   Use

   Golden gate / green gate compatible cloning vector

   Tags

   Linker, Myr, and YFP

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. L2132-FKBP-GPA-114-5-CyOFP1

   Plasmid

   #222891

   Purpose

   Biosensor chain detecting rapamycin and responding with BRET. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 5 GS linker, CyOFP1 protein.

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FKBP-GPA-20GS-NanoLuc114-5GS-CyOFP1
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. L2127-FRB-GPA-114-20-CyOFP1

   Plasmid

   #222886

   Purpose

   Biosensor chain detecting rapamycin and responding with BRET. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 20 GS linker, CyOFP1 protein.

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FRB-GPA-20GS-NanoLuc114-20GS-CyOFP1
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. L2129-FRB-GPA-114-5-CyOFP1

   Plasmid

   #222888

   Purpose

   Biosensor chain detecting rapamycin and responding with BRET. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 5 GS linker, CyOFP1 protein.

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FRB-GPA-20GS-NanoLuc114-5GS-CyOFP1
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. L2128-FRB-GPA-114-10-CyOFP1

   Plasmid

   #222887

   Purpose

   Biosensor chain detecting rapamycin and responding with BRET. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 10 GS linker, CyOFP1 protein.

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FRB-GPA-20GS-NanoLuc114-10GS-CyOFP1
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. L2131-FKBP-GPA-114-10-CyOFP1

    Plasmid

    #222890

    Purpose

    Biosensor chain detecting rapamycin and responding with BRET. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 10 GS linker, CyOFP1 protein.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FKBP-GPA-20GS-NanoLuc114-10GS-CyOFP1
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. L2130-FKBP-GPA-114-20-CyOFP1

    Plasmid

    #222889

    Purpose

    Biosensor chain detecting rapamycin and responding with BRET. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 20 GS linker, CyOFP1 protein.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FKBP-GPA-20GS-NanoLuc114-20GS-CyOFP1
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHW582

    Plasmid

    #198819

    Purpose

    In vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity. Based on GCaMP7, improved SNR, higher baseline fluorescence

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    15xUAS::GCaMP7b-SL2-mKate2::let-858 3'UTR
    

    Expression

    Worm

    Available Since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJR012

    Plasmid

    #198823

    Purpose

    Nucleolus-transport-enhanced Cre protein. Recombination-mediated gene expression knockout.

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    15xUAS::nCRE-SL2-GFP::let-858 3'UTR
    

    Expression

    Worm

    Available Since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHW539

    Plasmid

    #198811

    Purpose

    Dominant negative form of Protein Kinase A (PKA). Lowers PKA activity in targeted neurons.

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    15xUAS::PKA(DN)-SL2-GFP::let-858 3'UTR
    

    Expression

    Worm

    Mutation

    G310D mutation to abolish the binding of cAMP of …

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pD002

    Plasmid

    #198833

    Purpose

    Inhibition of synaptic vessel release due to disruption of synaptic vessel function by reactive oxygen species production.

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    15xUAS::miniSOG-103L-mCherry::let-858 3'UTR
    

    Expression

    Worm

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHW580

    Plasmid

    #198816

    Purpose

    In vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity. Based on GCaMP7, improved SNR, slow kinetics

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    15xUAS::GCaMP7s-SL2-mKate2::let-858 3'UTR
    

    Expression

    Worm

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Linked VLP NES

    Plasmid

    #182432

    Purpose

    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20-AcNES1 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al bioRxiv 2022.11.24.517869

    Inserts

    VP2C-FPPS-NES
    deltaVP1
    

    Use

    Synthetic Biology; Metabolic engineering

    Expression

    Yeast

    Promoter

    GAL1 and GAL10

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGG198

    Plasmid

    #165605

    Purpose

    Reporter plasmid for B1H-dependent expression of the HIS3/GFP operon with two hEGFP protospacers: PS1(upstream of promoter with 'CAGCG' PAM)-lac-PS2(downstream with 'CGGCG' PAM)-HIS3 and GFP

    Depositor

    Marcus Noyes

    Article

    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

    Insert

    hEGFP protospacer ('CGGCG' PAM) downstream of the HIS3/GFP promoter
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Secondary protospacer ('CGGCG' PAM) ins…

    Promoter

    lac

    Available Since

    July 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGG438

    Plasmid

    #165486

    Purpose

    Vector for expression of the SpCas9 VRKG variant in human cells: CMV-T7-humanVRKG(SpCas9, D1135V/S1136R/D1332K/R1333G)-1xNLS(SV40)-3xFLAG

    Depositor

    Marcus Noyes

    Article

    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

    Insert

    Mammalian codon-optimized Streptococcus pyogenes Cas9 VRKG(D1135V/S1136R/D1332K/R1333G)-1xNLS(SV40)-3xFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    D1135V, S1136R, D1332K, and R1333G mutations in S…

    Promoter

    CMV and T7

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. KIF13B

    Plasmid

    #163907

    Purpose

    Full length mouse KIF13B for purification from insect cells

    Depositor

    Martin Loose

    Article

    Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

    Insert

    kif13b (Kif13b Mouse)
    

    Tags

    Cleavable 6 x HIS tag

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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