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pcDNA3.1-Green_CaMBI

Plasmid

#124096

Purpose

Green calcium-modulated bioluminescent indicator

Depositor

Michael Lin

Article

Oh et al Nat Chem Biol. 2019 Apr 1. pii: 10.1038/

Insert

Green CaMBI

Expression

Mammalian

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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pRSETB-ro2GFP

Plasmid

#82366

Purpose

Inducible expression of redox sensitive GFP in bacteria

Depositor

S. James Remington

Article

Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

Insert

ro2GFP

Tags

6xHIS

Expression

Bacterial

Mutation

C48S/S65T/Q80R/S147C/Q204C

Promoter

T7

Available Since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti6.2-Renilla-ccdB

Plasmid

#87076

Purpose

Lentiviral Gateway-compatible expression vector backbone with an N-terminal Renilla luciferase

Depositor

Susan Lindquist, Mikko Taipale

Article

Taipale et al Cell. 2012 Aug 31;150(5):987-1001. doi:

Type

Empty backbone

Use

Lentiviral

Tags

Renilla luciferase

Expression

Mammalian

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pPTD-DRBD

Plasmid

#35622

Depositor

Steven Dowdy

Article

Eguchi et al Nat Biotechnol. 2009 Jun;27(6):567-71. E

Insert

PKR DRBD-1

Tags

6xHIS, HA, and TAT

Expression

Bacterial

Promoter

T7

Available Since

March 15, 2012

Availability

Academic Institutions and Nonprofits only
HS-H2B-RFP

Plasmid

#105953

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

H2B

Tags

RFP

Expression

Bacterial

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pSL1394

Plasmid

#129522

Purpose

pDEF-SpCas9::GFP, For deletion of pyuis4, PyBiP-minimal promoter, 3rd Generation

Depositor

Scott Lindner

Article

Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

Insert

SpCas9

Use

Plasmodium, rodent-infectious species

Tags

3XFLAG-NLS

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pTH1-SpSrtA

Plasmid

#64987

Purpose

Expresses the wild type of Streptococcus pyogenes sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

Insert

S. pyogenes sortase A

Tags

His6 tag, Mxe GyrA intein, and chitin-binding pro…

Expression

Bacterial

Mutation

deleted amino acids 1-81

Promoter

T7 promoter

Available Since

June 5, 2015

Availability

Academic Institutions and Nonprofits only

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plvx-AD8

Plasmid

#191558

Purpose

Inducible expression of HIV-1 Env

Depositor

Alon Herschhorn, Joseph Sodroski

Article

Herschhorn et al PLoS One. 2011;6(11):e26731. doi: 10.137

Insert

HIV-1 AD8 env

Use

Lentiviral

Mutation

None

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pTKhc-yibDp-GFP-cp6

Plasmid

#134938

Purpose

Low phosphate inducible circular permutation of GFP

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

GFPcp6

Tags

His tag

Expression

Bacterial

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HisFlag-CAD

Plasmid

#31615

Depositor

Aziz Sancar

Article

Lindsey-Boltz et al Nucleic Acids Res. 2004 . 32(15):4524-3

Insert

CAD

Tags

3xFlag, His, and T7

Expression

Mammalian

Available Since

Feb. 13, 2012

Availability

Academic Institutions and Nonprofits only
NFkBm2

Plasmid

#15090

Depositor

David Baltimore

Article

Taganov et al Proc Natl Acad Sci U S A. 2006 Aug 15. 1

Insert

miR-146a promoter

Expression

Mammalian

Mutation

2 NFkB binding sites mutated

Available Since

June 6, 2007

Availability

Academic Institutions and Nonprofits only
CytERM-dTomato

Plasmid

#98834

Purpose

In vivo visualization of the ER (can be used for colocalization studies and the OSER assay)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

CytERM

Tags

dTomato

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pCUPI NPD-GFP

Plasmid

#1191

Depositor

Susan Lindquist

Article

Patino et al Science 1996 Aug 2;273(5275):622-6.

Insert

N-terminus of Sup35 (SUP35 Budding Yeast)

Use

Cup1 promoter

Tags

GFP

Expression

Yeast

Mutation

fused N-terminal Sup35 (~1-253aa) to GFP

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pBAD-HaloGFP-Na2.4

Plasmid

#228470

Purpose

Bacterial expression of green fluorescent sodium ion indicator HaloGFP-Na2.4

Depositor

Robert Campbell

Article

Takeuchi et al RSC Chem Biol. 2024 Dec 3. doi: 10.1039/

Insert

HaloGFP-Na2.4

Expression

Bacterial

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCTcon2-5INS_EV3

Plasmid

#102293

Purpose

Yeast Surface Display of 5Ins_16_EV3

Depositor

Sarel Fleishman

Article

Baran et al Proc Natl Acad Sci U S A. 2017 Oct 10;11

Insert

Evolved Insulin binding scFV

Tags

3x(G4S) Linker, G3S Linker, HA, and Myc

Expression

Yeast

Promoter

GAL1

Available Since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only

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Atg24 1B

Plasmid

#197046

Purpose

His6 tagged Atg24 from S. cerevisiae for expression in E. coli.

Depositor

Michael Ragusa

Article

Reinhart et al Biochemistry. 2022 Apr 14. doi: 10.1021/

Insert

Atg24

Tags

6His-TEV

Expression

Bacterial

Available Since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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pSC101-GFPmut2-mScarlet-I TAG

Plasmid

#208188

Purpose

nonsense mistranslation, TAG stop

Depositor

Tanel Tenson

Article

Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

Inserts

GFPmut2
mScarlet-I

Use

Reporter

Expression

Bacterial

Mutation

Insertion 6TAG*(stop) 7AGT(Ser)

Promoter

Ptet+dnaK P1

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSC101-GFPmut2-mScarlet-I TGA

Plasmid

#208189

Purpose

nonsense mistranslation, TGA stop

Depositor

Tanel Tenson

Article

Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

Inserts

GFPmut2
mScarlet-I

Use

Reporter

Expression

Bacterial

Mutation

Insertion 6TGA*(stop) 7AGT(Ser)

Promoter

Ptet+dnaK P1

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSC101-GFPmut2-mScarlet-I TGATC

Plasmid

#208190

Purpose

nonsense mistranslation, TGA stop variant 2

Depositor

Tanel Tenson

Article

Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

Inserts

GFPmut2
mScarlet-I

Use

Reporter

Expression

Bacterial

Mutation

Insertion 6TGA*(stop) 7TCT(Ser)

Promoter

Ptet+dnaK P1

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSC101-GFPmut2-mScarlet-I T7

Plasmid

#208191

Purpose

frameshift mistranslation, T7

Depositor

Tanel Tenson

Article

Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

Inserts

GFPmut2
mScarlet-I

Use

Reporter

Expression

Bacterial

Mutation

Frameshift insertion 6TTTTTTT

Promoter

Ptet+dnaK P1

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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