We narrowed to 4,205 results for: LYC

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Myc-CD4-mCherry-HA-FRB

Plasmid

#100740

Purpose

To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

CD4

Tags

FRB, HA, Myc, and mCherry

Expression

Mammalian

Mutation

Engineered to remove large part of extracellular …

Promoter

CMV

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag4A-MITF-M (G244R)

Plasmid

#31155

Depositor

Yardena Samuels

Article

Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

Insert

MITF-M (MITF Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

Glycine 244 changed to Arginine

Available Since

July 22, 2011

Availability

Academic Institutions and Nonprofits only
pEGFP ratAPOBEC1 L173A/G227A

Plasmid

#167169

Purpose

Expresses rat APOBEC1 L173A/G227A in mammalian cells. Please note that this does not express GFP.

Depositor

Silvestro Conticello

Article

Chieca et al bioRxiv 2021

Insert

APOBEC1 (Apobec1 Rat)

Expression

Mammalian

Mutation

changed Leucine 173 to Alanine; changed Glycine 2…

Promoter

CMV promoter

Available Since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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miR-144 Trap

Plasmid

#60934

Purpose

to knockdown endogenous miR144 levels; lentiviral vector containing 8x copies of complementary miR-144-binding sites cloned 3' to GFP under the control of a biPGK promoter

Depositor

Curt Civin

Article

Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111

Insert

miR-144 Trap

Use

Lentiviral

Tags

GFP

Promoter

bidirectional phosphoglycerate kinase promoter (b…

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only
pDGE663

Plasmid

#153261

Purpose

M6E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE665

Plasmid

#153263

Purpose

M8E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE654

Plasmid

#153252

Purpose

M1E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDGB1omega2_SF (GB0466)

Plasmid

#68243

Purpose

Twister plasmid to swap inserts from an omega1 or omega1R vector to any alpha level vector

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Solanum lycopersicum intergenic region

Use

Synthetic Biology; Unspecified

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pDGB1alpha2_SF (GB0107)

Plasmid

#68233

Purpose

Twister plasmid to swap inserts from an alpha1 or alpha1R vector to any omega level vector

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Solanum lycopersicum intergenic region

Use

Synthetic Biology; Unspecified

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 27, 2015

Availability

Academic Institutions and Nonprofits only

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E1_pENTR_L1-L4_NI_G1397-DddtoxA-C

Plasmid

#171726

Purpose

ptpTALECD vector assembly, Entry clone 1 with attL1 and L4

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor

Use

Multisite gateway entry vector

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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E3_pENTR_L3-L2_NG_G1397-DddtoxA-C

Plasmid

#171734

Purpose

ptpTALECD vector assembly, Entry clone 1 with attL2 and L3

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor

Use

Multisite gateway entry vector

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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E3_pENTR_L3-L2_NG_G1397-DddtoxA-N

Plasmid

#171735

Purpose

ptpTALECD vector assembly, Entry clone 1 with attL2 and L3

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C- and N termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor

Use

Multisite gateway entry vector

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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E1_pENTR_L1-L4_NI_G1397-DddtoxA-N

Plasmid

#171727

Purpose

ptpTALECD vector assembly, Entry clone 1 with attL1 and L4

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor

Use

Multisite gateway entry vector

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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E1_pENTR_L1-L4_NN_G1397-DddtoxA-N

Plasmid

#171731

Purpose

ptpTALECD vector assembly, Entry clone 1 with attL1 and L4

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor

Use

Multisite gateway entry vector

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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E1_pENTR_L1-L4_NN_G1397-DddtoxA-C

Plasmid

#171730

Purpose

ptpTALECD vector assembly, Entry clone 1 with attL1 and L4

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor

Use

Multisite gateway entry vector

Available Since

July 27, 2021

Availability

Academic Institutions and Nonprofits only

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pNIR-Fb6m

Plasmid

#184680

Purpose

NIR-Fb to spike-Cov-19 RBD

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

NIR-Fb6m (S Synthetic)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pMS1088

Plasmid

#80159

Purpose

pET-Duet for bacterial expression of Cys-NuG2(4x)-Ald and co-expression of Mycobacterium tuberculosis Formylglycine Generating Enzyme

Depositor

Marcelo Sousa

Article

Walder et al J Am Chem Soc. 2017 Jul 5. doi: 10.1021/

Insert

HisSUMO-Cys-4xNuG2-Ald

Tags

HisSUMO

Promoter

T7

Available Since

Aug. 24, 2016

Availability

Academic Institutions and Nonprofits only

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DPAGT1 (DPAGT1A-c005)

Plasmid

#98228

Purpose

Baculovirus expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

DPAGT1A (DPAGT1 Human)

Use

Baculovirus

Tags

His-6-TEV

Mutation

pfam00953:Glycos_transf_4 98-275

Promoter

polyhedrin

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pDGE660

Plasmid

#153258

Purpose

M4E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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C513-E07: attB5r-<p10p-polyhedrinp>-attB1r

Plasmid

#162949

Purpose

Gateway attB5r/attB1r entry clone containing divergent AcMNPV polyhedrin and p10 promoters, for use in construction of polycistronic baculovirus expression vectors

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

dual baculovirus promoter cassette (AcMNPV p10 and polyhedrin)

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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